Celibate Hero: While this was frequent at the times in franco-belgian comics, Lucky Luke deserves a special mention in that he is explicitly stated to dislike the very concept of being in a relationship.
In the 2007 animated movie, Go West: A Lucky Luke Adventure there's a brief scene where he bonds with Native American Chief Crazy Wolf over the hardships of giving up smoking:
Crazy Wolf: Ooooh, Crazy Wolf finally gave up smoking! Lucky Luke: Yep, me too! Crazy Wolf: Did you suffer from many cravings? Lucky Luke: Yep — I had to chew on a piece of straw for a long time.
In the 2009 live-action movie, there's a short scene playing during the closing credits, where Lucky Luke is sitting with his back to the camera, smoking a cigarette. Then he realizes the camera is on him, does a double take, throws away the cigarette and replaces it with the familiar piece of straw before turning back to the audience and saying, somewhat sheepishly: "Hello! My name is Lucky Luke. I quit smoking back in '83. I feel much better now."
Lucky Luke's wisecracking horse and only partner to remain at the his side at all times.
Animal Talk: Can converse freely with any other animal in the series, and talks to himself (and by extension, the reader) a lot. Humans can't understand him, though Lucky Luke is hinted to at least get the general gist of it for the most part. In the 2009 movie, he's upgraded to Talking Animal.
Breakout Character: Very popular with the readers; he's starred in his own comics, both short gag stories and album-length adventures. In the Hanna-Barbera cartoon he has a larger role than in the comics, and tends to show up even in episodes based on albums where he didn't appear at all. In 2006 he even got his own animated series.
The Ditz: The only character in the comic stupider than Averell Dalton; he can't seem to get anything right.
Dogs Are Dumb: Just like Lucky Luke is faster than his own shadow, Rantanplan is stupider than his own shadow.
Dub Name Change: He's called Rin Tin Can in several English translations, and Rintindumb in others; and in the Hanna-Barbera cartoon, he's named Bushwack. In Scandinavia he's called Ratata.
One of the later album deals with her suffering an in-universe example of the trope, when a legend that she is supposedly a witch and demoness who came Back from the Dead as a ghost to haunt a city spreads throughout the west. It however turns out to be a Scooby-Doo Hoax created by a group of bandits who wanted to keep unwanted people away from a deserted town where they discovered a new gold mine. Naturally, Calamity Jane is not amused about her likeness being used as a "scarecrow" (as she puts it).
The Lad-ette: A G-Rated version of the trope; notably, her real life self played this trope straight.
Lethal Chef: Her cooking skills are so bad that at one point, a cowboy was willing to die rather than eat her cakes.
Unreliable Narrator: Like her real-life counterpart, she enjoys adding lots of juicy details when telling stories about her life, with a different version each time. She admits this when telling those stories to Luke.
Lucky Luke's most featured adversaries and the most iconic outlaws of the series. They are, in fact, not the "real" Daltons (Bob, Grat, Bill and Emmett, who appeared in one early story and were killed off at the end of it), but their identical, if more incompetent cousins.
Avenging the Villain: They started their career in an attempt to avenge the real Dalton Brothers' death at the hand of Luke. Their hate of Luke has become more personnal as the story has kept going on, though.
Breakout Characters: The original Daltons were lethal but one-shot villains who died at the end of their album; they ended up so popular that a new set of them were introduced, and ended up becoming as iconic as Luke himself.
Card-Carrying Villain: Their whole family considers crime as a tradition in the family. They take it to such extreme that their uncle, Marcel Dalton, is considered a Black Sheep just because he is the only honest member of the family.
Chronic Villainy: Any story about someone trying to redeem the Daltons (the Marcel Dalton story being the most notable example) is doomed to end up as a Shaggy Dog Story.
Continuity Snarl: Occasionally, for no reason whatsoever, William and Jack swap names. It happens first in their dÚbut story (for the first half, William is the shorter and Jack the taller; then it switches in the second half, making William taller and Jack shorter), and in some stories afterwards. In the 2006 movie and the 2010 animated series, the two brothers' names are likewise swapped.
Disappeared Dad: It's not clear what happened to their father, but he clearly isn't around anymore. Belle Starr has Ma Dalton mentioning a Noodle Incident about him using dynamite, suggesting he might be dead.
The Dreaded: As incompetent as they can be when they are pitted against Luke, they are dangerous enough to scare the crap of almost everyone else.
Even Evil Has Loved Ones: For all the time they argue with each others and Joe abuse his brothers, they always stick together and have each other's back. In one Book of the Rantanplan sin-off, when Averell get abducted, Joe is genuinely outraged against the Warden, and they escape for the sole purpose to bring him back.
Friendly Enemy: Inverted; Lucky Luke has few to no animosity toward them and tends to act nicely towards them, especially in later albums. When they get sentenced to death, he even tries everything he can to save them.
Idiot Ball/ Villain Ball: While Averell is the default holder, it gets passed around a lot among the four of them.
Joker Immunity: Averted with the original Daltons, but played straight hilariously with their cousins; no matter how many crimes they commit, and how many times Luke arrests them, they will always be merely sent to jail with a ridiculously high prison sentence (hundreds to thousands of years), where they will usually escape from nearly as soon as they arrive due to the wardens being complete morons; this takes such proportions that it gets more and more lampshaded as the series goes on. In later albums, Luke ends up sick of having to run after them again and again, and calls out the wardens for their incompetence.
They actually get sentenced to death in a later album, but still find a way to escape it eventually.
Not-So-Harmless Villain: When first introduced, the new Daltons were portrayed as so incompetent Luke was actually eager to meet them again because he found them entertaining. They eventually took lessons and became as dangerous as the original Daltons, as long as they weren't confronted with Luke himself.
And even now, despite their stupidity, they are shown to be actually quite dangerous. They did come close to killing Luke on occasions.
Suspiciously Similar Substitutes: For the original Daltons. Morris regretted killing off the original Daltons, and RenÚ Goscinny had liked the original Dalton Gang story so much that when he took over the writing for the comic, he introduced another quartet of Daltons and billed them as the cousins of the original Daltons.
Training from Hell: The Dalton Brothers start out as incompetent villains incapable to do anything right so they grind themselves through a brutal training regime. They end up becoming great riders, deadly shooters and very competent in general, despite their stupidity.
Villain Protagonists: In several stories the focus in more on their attempts to outwit Lucky Luke than it is on Luke himself. They've also appeared solo in several stories, and like Rantanplan, have starred in their own animated series in 2010.
Voiced by: Johan Hedenberg (Swedish)
The oldest, but shortest, of the brothers and the mastermind of their various schemes and prison breaks.
Big Brother Bully: To Averell. Though to be fair, his anger and brutality usually is provoked by Averell's stupidity more than anything. Otherwise, it has been shown he does care about all his brothers, Averell included.
Book Dumb: He is by far the smartest of his brothers, but he is completely uneducated and unfamiliar with anything you'd learn to school. To give an example, he thinks Christopher Colombus was the first authentic American.
Jerkass Has a Point: When he explains to the Natives the effect the people of Daisy Town will have on them.
Also, in the Rantanplan spin-off, he at one point calls out the Warden for failing not only to prevent them from escaping, but also to prevent people from entering in the Penitentiary to abduct prisoners. While the scene is played for laughs, the Wardens do suck at their job.
Genre Savvy: Sometimes points out obvious details that go over Joe's head.
The Heart: Another role he shares with Jack. As such, they constantly need to keep Joe off Averell's throat.
Hoist by His Own Petard: In his confrontation with Luke, having only one bullet left, he tried to defeat him by playing the Russian Roulette with him. Needless to say, Luke, being Born Lucky, easily survived the first round and easily tricked him into believing he was losing so he could take him without killing him.
Butt Monkey: He usually is the one who takes hits when Joe is pissed off.
The Brute: He originally was introduced as the physically strongest of the the four. He still displays shades of this occasionally in later books, but for most he is essentially portrayed as the stupidest.
Averell: *crunch* I really like foreign cooking! What's this delicious crust around the frijoles? Emilio Espuelas: That's called a terracotta bowl, amigo.
Minion with an F in Evil: To the point that when first introduced, he had a "Not Wanted" poster instead of a "Wanted" one. He did act a bit meaner after his training with his brothers, but later books turn him back into a borderline Stupid Good character.
Momma's Boy: He has always been Ma Dalton's favourite son.
Not-So-Harmless Villain: One episode of the Rantanplan series has two scientists kidnapping him and using him as a test subject for a formula attempted to make him intelligent. This ends up turning him into an EvilGenius Bruiser who actually was even more dangerous and competent than Joe, to the point the leading scientist ended up turning him back to normal and destroying his formula.
Pet the Dog: Literally; he has a soft spot for Rantanplan and is, on the whole, quite kind to him.
Aged mother of Joe, William, Jack and Averell.
Anti-Villain: She isn't actually villainous, and any times she will play an antagonistic role, it usually is out of love for her children. Luke actually is in decently good terms with her otherwise.
Beware the Nice Ones: Unlike her sons, she is a genuinely nice and kind person... As long as her boys aren't in danger.
The Dreaded: Exaggerated; in his first appearance, he scared the crap out of people so much that nobody dared arrest him or put him on trial. Later, in one book, he manages to rob people just by leaving a sign stating he is around. This causes people to leave their goods in front of the sign for him to take when he will back.
Literal-Minded: He took the concept to "steal from the rich to give to the poor" a bit too literally; whenever he gives money to a poor, that person instantly becomes rich in his eyes, causing him to steal from him. He ends up using a Loophole Abuse to share the money with his brother, by them taking turns in playing the "Poor" role.