* ''WebAnimation/TheNekciMenijShow'' has all of its female characters jockeying around each other for this position in the music world. Beyonce is the most obvious example of an Alpha Bitch, based on her ostentatious behavior and her need to command every room she's in.
* ''TheMostPopularGirlsInSchool'' is basically ''Alpha Bitch: The Web Show''. Mackenzie Zales, Shay van Buren, and Tanya Berkowitz are all the Alpha Bitches of the Overland Park Cheer Squad, the van Buren family, and the Atchison Cheer Squad respectively.
* ''WebAnimation/TeenGirlSquad'', being a parody of high school stereotypes, naturally features one, [[TheCheerleader appropriately nicknamed Cheerleader]]. Who, as we eventually learn, isn't even a real cheerleader. She's also nowhere near as popular as this trope usually entails.
* ''WebAnimation/{{RWBY}}'': Weiss Schnee's characterization in a nutshell. At least initially. She [[CharacterDevelopment gets]] [[JerkWithAHeartOfGold better]], though.