* FoeYay:
-->'''Vampire!Joker:''' Batsy... You complete me.
* InferredHolocaust: Vampire Joker and Batman have a brawl in a blood bank, Joker drinking gallons of it and even more being spilled in the process. Considering blood supplies are in short enough supply in the real world, that means hundreds, if not thousands, died because of lack of transfusions.
* HilariousInHindsight:
** This wouldn't be [[Film/TheDarkKnight the last time]] the Joker told Batman [[Film/JerryMaguire "You complete me."]]
** It also wouldn't be the last time Batman gives some of his own blood to Joker [[spoiler:in order to cure him of a type of virus. Something very similar to that happens in the ''Batman: Europa'' comic and [[VideoGame/BatmanArkhamCity Batman: Arkham City.]]]]
* {{Narm}}: The "reveal" that {{Alucard}} spelled backwards is... DRACULA!
* WhatDoYouMeanItsForKids: While this movie is still made for general audiences, this movie is considerably darker and bloodier than the ''WesternAnimation/TheBatman main series'' . Toonami mistakenly gave it a TV-Y7 rating during its first airing and only later upgrading it to a (much better fitting) TV-PG.
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