* AccidentalNightmareFuel: Ed's mouth flaps sometimes cross into this territory, due to the movement [[UncannyValley not quite]] matching up with the speech.
* BizarroEpisode: The episode "Mako" revolves around an animated alien who comes to earth and takes over the bodies of whoever it goes inside. Its victims have no memory of what happens when the alien takes them over.
* CrowningMomentOfFunny:
** In one episode, Wilbur takes up painting and hires a ''gorgeous'' model as a bid to get Carol's attention. He brings her into the barn, where she announces that she needs to change. Wilbur modestly closes Ed's stall door...[[CovertPervert while Ed strenuously tries to keep it open.]]
* EarWorm: The theme song is the reason most people remember this show.
* EpilepticTrees: It's possible to interpret ''Mister Ed'' as the deranged hallucinations of a man who owned a completely normal horse, but in-universe evidence that Ed ''can'' talk is much stronger.
* HilariousInHindsight: If you watch the series carefully, you'll notice that Creator/AlanYoung is quite skilled at doing foreign accents. [[WesternAnimation/DuckTales Guess who he had to do a Scottish accent for many years later]]?
** On episodes has him playing the bagpipes and mentioning that he's Scottish.
** It's even more hilarious when he calls a character "Mr. Scrooge" in a Christmas-themed episode.
** Not to mention the fact that Creator/AlanYoung had to do a Scottish accent [[ActingForTwo when he played Wilbur's father]].
* RetroactiveRecognition:
** Creator/AlanYoung as Wilbur Post.
** Long before he played Mr. Addison, Larry Keating previously played Harry Morton on ''Radio/TheBurnsAndAllenShow''.
** In addition to their work on ''Mister Ed'', theme song composers Jay Livingston and Ray Evans also composed the theme song to ''Series/{{Bonanza}}'' as well as the pop songs "Buttons and Bows", "Mona Lisa", "Tammy", "Silver Bells", and "Que Sera, Sera (Whatever Will Be, Will Be)".
** Ben Starr wrote 40 episodes. Starr is best known for co-creating ''Series/SilverSpoons'' and co-developing ''Series/TheFactsOfLife''.
* TearJerker: An episode featuring SpecialGuest Zsa Zsa Gabor saw Gabor (playing herself) come to town to film a movie. The studio borrows Ed as a horse for Zsa Zsa to ride in the film; when she falls in love with Ed and refuses to ride any other horse, the movie studio offers Wilbur an enormous sum of money to buy Ed outright. Wilbur initially balks but Ed, realising how much the money would do for Wilbur, pretends he is star-struck and demands to be allowed to leave. Wilbur accedes and helps Ed pack up his things with tears streaming down his face as he does so.
* TheyCopiedItSoItSucks: Some viewers found this a blatant ripoff of several films in which Donald O'Connor had adventures with "Francis the Talking Mule."
* TookALevelInJerkass: Mister Ed becomes more demanding and more inclined to get Wilbur into trouble as the series continues on.
* UrbanLegendOfZelda:
** Snopes.com, for a joke, posted a page that Ed was ''really'' played by a female zebra named Amelia.
** A popular rumour (referenced in the movie ''Film/DrDolittle''), which may or may not have been started by Alan Young, was that in order to make Ed "talk" the production crew smeared peanut butter on the horse's gums. In actuality, the effect was initially pulled off by pulling on a nylon thread in the horse's mouth; however, eventually the horse learned to flap its lips on its own when someone touched its hoof. The horse soon figured out to simply wait until Young stopped talking before "talking" back, with no further cues necessary.
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