Plus Gerda (un)helpfully pointing out when your health is running low.
Anti-Climax Boss: After reiterating the entire first stage again with one different mid-boss (itself a reuse of one from 'Splosion Man), Smiley takes on Brad in his Bradcopter and the Bradbots at once. He isn't as challenging as the previous major boss, Mutant Hehe, and after taking down his vehicle the final fistfight with him is laughably easy.
Ear Worm: Twisted Pixel continue their tradition of these with style, most notably in the case of Brad's Theme.
Smiley lampshades this by telling Star that they're gonna go back to the therapist if he keeps the trope up. The developers outright state that he's not right in the chest cavity in the description of one of the bonuses.
Most Annoying Sound: Gerda when pointing out the obvious, not too mention informing you how bad you are at the game. "Captain, you have 75% health. You have less than half your health. Captain, you have less than 25% health. Stop Sucking."
The Scrappy: Gerda. The constant "Your health is now...<insert insult here>" is usually one of the main criticisms aimed at the game. Admit it, when Smiley left her in the bank to die again, you delighted at the prospect of her silence. Alas she still talks afterward.
Surprise Difficulty: The game is harder than it looks, with the difficulty really ramping up in the Silver Age and Manga levels. But as mentioned several times on this page, you've got infinite continues. The optional Challenges are by far the hardest thing in the game, as you've only got one hitpoint in them.
That One Boss: Mutant Hehe. Not only does he have a large health amount and has some pretty hefty attacks, the girls' magic restore his health to full three times, making Hehe a Marathon Boss as well.
Values Dissonance: In the "Improbable Paper Pals" stage, Star eventually complains about how he's not allowed to swear, but the comic is allowed to parade around politically incorrect stereotypes, like a Straw Feminist super-villain and Paper Lad's Japanese stereotype partner.
It's a pretty blatant Take That to the hypocrisy of the Comic Codes of the time, which didn't allow brutal violence or cursing, but was A-OK with stereotypes running around.