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Tear Jerker: 2001: A Space Odyssey
Amazingly, HAL's death scene.
Now with video. "Stop, Dave. I'm afraid." and of course, the eponymous portion at 3:48 - "Daisy, Daisy... give me you-r... answer... do."
After the discussion of HAL's malfunction, his having killed the rest of the crew, and having heard his calm-in-a-creepy-as-hell-way voice for the whole movie, this Wham Line points out that, somewhere deep inside, he's as human as any of us and just as afraid to die.