Super Rei's death at Black Mari's hands. Although it's highly foreshadowed that the Return To The Past may resurrect her, it still hurts to read her Go Out with a Smile as Asuka holds her and begs her to stay alive, and then her body getting lost in the midst of all the rubble as Mari brings down a building on top of them both (killing many bystanders deliberately). This, Mari treating Rei like an Expendable Clone, and then threatening to rape and kill Asuka's other lover Ranma, drop-kicks Asuka through the Rage Breaking Point and makes her pummel Mari while screaming out oaths of revenge. Later on Submariner R arrives as The Cavalry after Asuka beats Mari up, and he asks where Rei is... cut to them arriving to the rendezvous point with a hefty amount of grief.
Giganta Asuka grabs on to Kiki and squashes her into pure "chunky salsa" before tossing the bloody mess to Prime and stomps on a boat full of people for the sake of pulling a Kick the Dog, then gloating about how the Young Avengers won't kill her because of super-heroic morality. Mr. T orders the YA to look away before beating her to a very literal inch of her life, and Chris pretty darkly says that he hopes she gets the death penalty.
Extra-TERROR-Estrial Thrills and Chills:
The events of Aliens are still a major Trauma Button for former Colonial Marine Jeanette Vasquez. She spends a good chunk of her initial contact with the hive having flashbacks to the clusterfuck that took most of her squad.