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Tear Jerker: Kuroshitsuji
The deaths of Lau and Abberline in episode 20.
The very beginning of episode 22 is pretty sad, too. Maria, Abberline's to-be wife, reads about his death and gets a handful of toys as compensation. Poor Maria...
Freckles. Freckles, Freckles, Freckles. Dear God. Freckles. After that particular piece of nastiness, Ciel's grasps at redemption were pretty sad to see. "Would you like an orange, sir?"
In chapter 44, watching Maylene and Finnian's breakdown after it seems to seriously and completely dawn on them that Sebastian is dead is utterly heartbreaking. Luckily, Sebastian is not really dead.
Episode's 7 and 8 of the second season in which Alois, who before seemed like a complete jackass, is stabbed and ignored by Claude for a bit who's busy practically orgasming over the taste of Ciel's blood. Alois's tearful response "Oh, you look like you've seen a bunch of maggots squirming in a dung pile" to Claude's indifference towards him, we learn his backstory where his little brother died and left him alone, and he practically confesses his love for Claude, telling him he's the only one he has left in the world, before Claude tenderly holds his face and stabs him to death.
Episode 11 Alois(in Ciel's Body) asking Hannah to tell him that she loves him and give him a hug. Which he's ALWAYS NEEDED. She does both, which prompts him to cry tears of joy and say "Love is...warm, isn't it?" *sniff* Pass the tissues please.