Here a wick check will be performed for Little Sister Heroine.
Why?: A discussion in the TV Tropes meta thread in Yack Fest indicated that this trope is both a possible "in Japan" duplicate of Brother–Sister Incest, as well as a magnet for creepy examples.
Wicks checked: 0/50
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Non-creepy usage that overlaps with Brother-Sister Incest (0/50)
Non-creepy usage that does not overlap with Brother-Sister Incest (0/50)
Creepily-written examples (0/50)
Other usage (0/50)
ZCEs and unclear (0/50)