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Nightmare Fuel: Cannibal Holocaust
Definitely, though the making of the film is just as disturbing.
Hailed as one of the most disturbing films ever made, it contains scenes of rape, mutilation, dismemberment, cannibalism, and rapes all senses. What's more, real animals were killed onscreen, and footage of real executions were used, convincing the brain that what they are seeing may in fact be real. This isn't helped by the film being in clear daylight with all the bright colours of the rainforest.
The creepy music being used at inappropriate times, such as the gentle, almost happy theme song being used in the scene of a woman being decapitated.
It was considered so realistic that the Italian government thought the filmmakers had made an honest-to-god snuff film, and it took producing the very much alive actors to prove that it wasn't. They had to demonstrate in court how they'd faked the impalement scene.
The scene of Felipe having his leg cut off and they attempt to Heal It With Fire and that final shot of the clip with Mark giving that look at the camera that is between fascination and bliss at the blood.