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Reality Subtext: A number of Beatrix Potter's stories contain this, but The Tale of Mrs. Tittlemouse is a special case. Potter's work had become quite popular and she was frequently receiving unannounced and uninvited guests. She wrote this story as a way to get the point across that such things were quite frustrating to her.
Sacred Hospitality: Mrs. Tittlemouse seems to believe in it, given how polite (and ineffectual) she is when trying to get uninvited guests to go away. By the end of the story, however, she puts her foot down.
Spiders Are Scary: Averted — in this story, the spider is just a mild nuisance, albeit a polite one.
The Thing That Would Not Leave: Mr. Jackson, the toad. Eventually Mrs. Tittlemouse gets so fed up with him that she makes her doorway too small for him to get in.