[[folder: W.O.O.H.P.]]

-->'''Voiced by:''' Jennifer Hale
Samantha "Sam" has long red hair, emerald green eyes and a green catsuit. She is the most intelligent and practical of the group.

* ActionGirl
* AlliterativeName: In one episode, she presents herself as "Samantha Simpson". Okay, they were undercover, but they always use their own names for these situations, so why not?
* BadassAdorable
* BadassBookworm
* BareYourMidriff: She generally bares her midriff in larger amounts than Clover and Alex, but not as frequently.
* BeautyBrainsAndBrawn: The Brains.
* BewareTheNiceOnes
* BlondeBrunetteRedhead: The Redhead.
* BraidsOfAction: Temporarily, during the Gladiator episode.
* BrainwashedAndCrazy: A RunningGag in the show is that she winds up like this on a routine basis.
* DeadpanSnarker
* ExtremeOmnivore: Eats a hot towel while at a sushi restaurant, and seems to enjoy it. However, in her defence, the villains had recently stolen her intelligence with their StupidityInducingAttack, so she at least ''thought'' it was food.
* FieryRedhead
* FreudianTrio: The Superego to Clover's Ego and Alex's Id.
* HollywoodNerd
* {{Hypocrite}}: One Season 3 episode has her acting like a super neat-freak and constantly nagging the girls to clean up the apartment. At the end of the episode we see her room, which is a dump that shows no signs of ''ever'' having been cleaned.
* IntrepidReporter: The major that Sam pursues as of Season 6.
* TheLeader
* NerdsAreSexy: Has this appeal InUniverse and out.
* NiceGirl
* OnlyOneName: She's mostly referred to as just "Sam." However, see the AlliterativeName entry above for how this might be subverted.
* OnlySaneWoman: Most of the time.
* RapunzelHair: Her hair reaches to her waist.
* RedHeadedHeroine: One of the heroes and has red hair.
* RedheadInGreen: In her spy outfit.
* SignificantGreenEyedRedhead
* TeamMom: She's generally the most mature and responsible of the main three.
* TheSmartGirl
* TheSpock
* TomboyishName
* TownGirls: The Neither to Clover's Femme and Alex' Butch.
* TVGenius: Some episodes give her shades of this.

-->'''Voiced by:''' Andrea Baker
Clover has medium blonde hair, blue eyes and a red catsuit. Probably the show's most stereotypical character, Clover is a shopping addicted, boy crazy, teenage "fashionista".

* ActionFashionista
* ActionGirl
* ArchEnemy: Geraldine, and arguably Mandy. Sometimes she has a friendly rivalry with Sam.
* AllergicToRoutine: Clover usually gets bored easily. Perhaps this may explain why she has an obsession with boys…
* AmbitionIsEvil: Clover usually tries to get famous. In fact, such a behavior is strongly explicit during the episode "Model Citizens" in which Clover and Mandy compete in a beauty contest. There are many other examples though.
* BadassAdorable
* BalloonBelly: She gets pretty fat in "Passion Patties", after gorging herself on Ms. Bittersweet's cookies. The only way to get rid of the fat was to use an antidote.
* BeautyBrainsAndBrawn: The Beauty.
* BerserkButton: Don't dare to say that Clover uses clothes bought in a liquidation. It never ends well… She doesn't like being turned down by a guy as well.
* BlondeBrunetteRedhead: The Blonde.
* ButtMonkey
* ClothingDamage: This happens A LOT to Clover. This is especially explored during "Attack Of The 50 Foot Mandy".
* CorruptTheCutie: This happens to Clover's intern, Tara, due to Clover's influence. She's not seen recovering.
* CallToAgriculture: She had to visit a farm during "Spies on the Farm".
* {{Cloudcuckoolander}}: Sometimes she gets lost in her dreams, specially during missions.
* DeadlyUpgrade: She becomes a super agent during "Super Agent Much?". Do you want more?
* DumbBlonde: Sometimes. She's usually smarter than she lets on.
* EverythingSoundsSexierInFrench: Occasionally does this.
* TheFashionista: Pursues fashion design as a major as of Season 6.
* FreudianTrio: The Id to Sam's Superego and Alex's Ego.
* UsefulNotes/FurryFandom: Clover turning into a furry cat? The walking, talking, human animals in the episode Animal World?
* GirlyBruiser: Clover plays the trope the straightest as she's more of a girly girl than the others.
* GoodBadGirl
* GoKartingWithBowser: This happens many times with Clover. For example, during the episodes "Sis-KaBOOM-Bah!" and "Evil Sorority" when she "fights" alongside with Mandy like… [[LesYay uh… best friends]]?
* GranolaGirl: Stated to be a vegetarian, but her love of fish and seafood would technically make a "pescetarian" (a semi-vegetarian that eats fish and seafood).
* GratuitousFrench: Quite evident.
* HormoneAddledTeenager
* IHaveYouNowMyPretty: Happens to Clover way too many times to count.
* JackOfAllStats: Clovers suits well here. She is strong, but not the strongest. She is smart, but not the smartest. In fact, even Jerry had problems to justify her presence in the team (during the episode "Like, So Totally Not Spies").
* JerkWithAHeartOfGold
* LaserGuidedAmnesia: Too many times to count.
* LovableAlphaBitch: In contrast to Mandy, who was more of a classic AlphaBitch.
* LovableSexManiac: She is not an extreme example like a certain character from a recent anime by Gainax ([[Anime/PantyAndStockingWithGarterbelt Anarchy Panty]]), but she seems to have a natural tendency for love.
* TheMcCoy
* OnlyOneName: As far as we know…
* RapePillageAndBurn: During the episode "Evil Sorority".
* SatelliteLoveInterest: A lot of her {{Temporary Love Interest}}s were this.
* SitcomArchNemesis: Has this relationship with Mandy.
* SkewedPriorities: Sometimes. Clover finds herself caring more about fashion and boys than about the missions she and the girls go on.
* SpaceWhaleAesop: One episode has Clover declaring that it's better to have a big head than to have a big everything else.
* SpoiledSweet: Yes, CaptainObvious!
* StrappedToAnOperatingTable: In "Starstruck".
* TalksLikeASimile: From time to time.
* TemporaryLoveInterest: Practically every episode she has one.
* TomboyAndGirlyGirl: The Girly Girl to Alex's Tomboy.
* TownGirls: The Femme to Sam's Neither and Alex' Butch.
* {{Tsundere}}: Type A, sometimes.
* ValleyGirl
* ZergRush: Clover was leading the fandom of Ricky Mathis during the episode "A Thing For Musicians" in a very dangerous way.

-->'''Voiced by:''' Katie Leigh (Season 1-2), Katie Griffin (Season 3-4)
Alexandra "Alex" has short black hair, tan skin, light brown eyes and her spy catsuit is yellow. Alex is the most naive and innocent of the group, and she's interested in sports and athletics.

* ActionGirl
* {{Adorkable}}
* AmbiguouslyBrown
** Maybe not as ambiguous as first thought--some fans have examined screenshots of her parents, and have come to the conclusion that Alex's mom is at least half if not fully Hispanic, while her dad looks half-white/half-Hispanic.
** Season 6 retcons her dad into being similar in appearance to her mother, thus completely shattering this trope.
* BadassAdorable
* BeautyBrainsAndBrawn: The Brawn.
* BewareTheNiceOnes
* [[TheBigGuy The Big Girl]]
* BlondeBrunetteRedhead: The Brunette.
* ButtMonkey: At times, mostly because of her clumsiness and naivete.
* {{Cloudcuckoolander}}: Sometimes.
* CuteClumsyGirl
* TheDitz
* DrivesLikeCrazy: Though she isn't ''too'' bad, she had a lot of trouble passing her driver's exam and is generally considered the worst driver of the three. Ironically however, she is often the one to drive.
* FreudianTrio: The Ego to Sam's Superego and Clover's Id.
* FriendToAllLivingThings: She actually wanted to grow up and either be a veterinarian or study animal physiology.
* GirlsLoveStuffedAnimals: Her oldest friend is a toy turtle named Ollie and she has a teddy bear backpack.
* InSeriesNickname: Her full name is "Alexandra", but everyone call her "Alex".
* KindlyVet: The major that Alex pursues as of Season 6.
* TheKirk
* TheKlutz
* MixedAncestry: Alex is half-Caucasian and half-Hispanic.
* NiceGirl: By far on the nicest characters in the series.
* OnlyOneName: At least up until season 6, where her father, "Dr. Cassoi", makes a second appearance.
* PassionateSportsGirl: The most athletic of the three.
* SpoiledSweet: Alex is probably the most straight-up example of the three.
* SweetTooth: Frequently played for laughs in the 6th season.
* {{Tomboy}}
** TomboyAndGirlyGirl: The Tomboy to Clover's Girly Girl.
** TomboyWithAGirlyStreak: While she's obviously the most tomboyish of the main three, Alex is also really into fashion and boys, sometimes to the same extremes as Clover.
** TomboyishName
* TownGirls: The Butch to Sam's Neither and Clover's Femme.
* TrademarkFavoriteFood: She loves Asian cuisine--especially Chinese food.
** Her favorite type of muffin is natural bran.

-->'''Voiced by:''' Lindsay Ridgeway (Season 2-3), Stephanie Broschart (Season 3-5)
Introduced in Seasons 2–3 as the newest recruit to the team.

* AlwaysSomeoneBetter: To Alex in her first appearance.
* [[SixthRanger Fourth Ranger]]
* JackofAllStats: Probably one of the straightest examples--Britney's good at lots of things, but she doesn't necessarily excel in anything.
* OnlyOneName
* PutOnABus: The only reason she's not a main character. This of course leads to...
* TheBusCameBack: ''Three times'' even!
* TokenMinority
* TrueBlueFemininity

-->'''Voiced by:''' Jennifer Hale
Sam's mother.

* BlondeBrunetteRedhead: The Brunette in "Mommies Dearest", later the Redhead in "Totally Busted".
* BrainwashedAndCrazy: In "Mommies Dearest".
* HappilyMarried: The B-plot of "The Wedding Crasher" has Sam looking for a gift for her parents' 20th wedding anniversary.
* MyBelovedSmother: She's established as being overprotective of Sam.
* RapunzelHair: Reaches down to her waist like her daughter.
* RedheadInGreen: In her spy outfit but to a lesser extent than Sam as it's a jade green.
* StrongFamilyResemblance: Like with Clover's mom, Stella, and Alex's mom, Carmen, Gabby looks identical to Sam, just older.
* TookALevelInBadass: Trains for and becomes a full time spy in the Season 4 finale.

-->'''Voiced by:''' Kath Soucie (Season 2), Andrea Baker (Season 4-6)
Clover's mother.

* BlondeBrunetteRedhead: The Blonde in between "Mommies Dearest" and "Totally Busted".
* BrainwashedAndCrazy: In "Mommies Dearest".
* HospitalHottie / TheMedic: Revealed to be a successful ear surgeon in Season 6.
* MsFanservice: Spends the majority of "Mommies Dearest" in a swimsuit.
* OpenMindedParent: At the end of "Nine Lives" upon learning that Clover wants to be a fashion designer instead of an ear surgeon.
* StrongFamilyResemblance: Like with Sam's mom, Gabby, and Alex's mom, Carmen, Stella looks identical to Clover, just older.
* TookALevelInBadass: Trains for and becomes a full time spy in the Season 4 finale.

-->'''Voiced by:''' Katie Leigh
Alex's mother

* BlondeBrunetteRedhead: The Redhead in "Mommies Dearest, later the Brunette in "Totally Busted".
* BrainwashedAndCrazy: In "Mommies Dearest".
* IWantGrandkids: A mild example. When she's not upset over Alex's grades, she's encouraging her to look for a boyfriend.
* ShipperOnDeck: To any guy she thinks her daughter likes.
* StrongFamilyResemblance: Like with Clover's mom, Stella, and Sam's mom, Gabby, Carmen looks identical to Alex, just older.
* TookALevelInBadass: Trains for and becomes a full time spy in the Season 4 finale.

!!Gerald James "Jerry" Lewis
-->'''Voiced by:''' Jess Harnell (Season 1-2), Adrian Truss (Season 3-6)
The founder and administrator of W.O.O.H.P., the World Organization Of Human Protection, Jerry is responsible for briefing the girls on their missions.

* AccidentalPervert: A milder example, but one episode has him summon the girls early in the morning and recording their interactions with a camcorder. At one point, he does a [[MaleGaze close up]] on [[MsFanservice Sam]], who happens to be in a ''[[ModestyTowel towel in the process]]''. Jerry is quite embarrassed over it.
* AuthorityEqualsAsskicking
* BadassGrandpa
* CainAndAbel: The Abel to Terrence's Cain.
* {{Catchphrase}}: "Oh, my!" and "Ta-ta for now!", amongst others.
* ColonelBadass
* CoolOldGuy
* CrazyPrepared
* DeadpanSnarker
* JerkWithAHeartOfGold: Does ALOT of stuff to the girls ForTheLulz, particularly his methods of transporting them, but has also saved their lives a number of times.
* TheMentor
* MyGreatestFailure: Selling his brother out when they got caught cheating on an exam.
* ParentalSubstitute: He acts as a parental figure to the girls and, at times, scolds them when they messed up. They even share plenty of heartwarming moments.
* ThemeTwinNaming: "Terry" and "Jerry". Oh, and the [[WesternAnimation/TheAmazingSpiez spin-off]] reveals they have a sister named ''Sherry''.

[[folder: L.A.M.O.S]]

!!Terrence Lewis
-->'''Voiced by:''' Matt Charles

* AlwaysIdenticalTwins: The reason why he doesn't look like Jerry is because he had plastic surgery performed.
* BigBad: For Season 4, anyway.
* CainAndAbel: The Cain to Jerry's Abel.
* EvilBrit: And an actual Brit.
* EvilTwin: To Jerry.
* MeanBrit
* ThemeTwinNaming: His nickname is "Terry".

!!Tim Scam
-->'''Voiced by:''' Michael Gough

* CardCarryingVillain: In his first appearance, Clover calls him out on the scumbag he is. His response? A small "Thank you." To be fair, he's far from the only villain in the show who exhibits this behavior.
* CoDragons: With Boogie Gus and Helga Von Guggen in Season 4.
* EvilGenius
* {{Greed}}: His main motive. Averted in his first appearance, where he wanted to [[OmnicidalManiac drain the Earth of its water supply]], which would have undoubtedly killed all life on the planet (including himself).
* {{Hypocrite}}: Feels that WHOOP never appreciated his genius and the technological advances he brought to the organization, despite the fact that he used said technology for his own selfish interests.
* RecurringCharacter: Has made more appearances than any other villain in the series to date, including Terry.
* TheSociopath: Very likely, considering that his reasons for becoming evil aren't ''as'' petty as most of the other recurring villains. Most of the others suffer from (rather minor) disappointments that cause them to snap. Scam, however, is depicted as perfectly sane (for the most part) and practically kills people for its own sake.
* SdrawkcabAlias: Mac Smit.
** This trope actually becomes a plot-point in one episode, where they finally realize who he really is because they see his name-tag reflected backwards in a metal panel.

!!Boogie Gus
-->'''Voiced by:''' Joe Motiki

* AlmightyJanitor: He was a janitor at WOOHP before becoming a villain.
* CoDragons: With Tim Scam and Helga Von Guggen in Season 4.
* DiscoDan: Is he ever.
* FunnyAfro
* MakeWrongWhatOnceWentRight: His first appearance had him travel back in time to become Jerry's friend and convince him to create an evil version of WOOHP called the "World Organization of ''Harming'' People."

[[folder: Others]]

-->'''Voiced by:''' Jennifer Hale

* AlphaBitch
** LovableAlphaBitch: See JerkWithAHeartOfGold below.
* AnnoyingLaugh: Mandy has a ''really'' annoying laugh. It sounds like a goat's bleating.
* BeautyMark
* BigBad: Once as a FutureBadass where she nearly took over the world. And of the one hour ''Totally Busted'' special when she and her lackies get infected by a shampoo that makes them "spy hunters" bent on killing Sam, Clover and Alex.
* DamselInDistress: She gets mistaken for Clover and kidnapped in one episode, resulting in the Spies having to bail her out. Likewise a Halloween episode had the spies have to save her from zombies ''which she herself had accidentally summoned''.
* EvilCounterpart: Not "evil" per se, but when you ''really'' think about it, Mandy's essentially Clover without any of her redeeming qualities.
* GracefulLadiesLikePurple: Purple is her signature color and is always seen wearing it.
* {{Jerkass}}
** JerkWithAHeartOfGold: Surprisingly, Mandy has shown the capacity to be a nice person, but it never lasts long and she always goes back to being mean again if she's provoked in some way.
* OnlyOneName: It's never revealed what Mandy's last name is.
* PopularIsDumb: It's implied (and proven) that Mandy does poorly in school. She actually got ''straight Fs'' on her report card at one point, [[spoiler:and the report was originally thought to be Alex's, which had As and Bs on it, but it turned out that there was actually a mix-up at their school]].
** [[spoiler:And yet she somehow managed to graduate from high school and go to college]].
* PurpleEyes
* RedOniBlueOni: The loud and aggressive Red Oni to her cousin Mindy's calm and collected Blue Oni.
* RichBitch: It's been said (and shown) that Mandy comes from a very wealthy family.
* SitcomArchnemesis: To all the girls, but mainly with Clover.
* TalkingToHerself: With Sam--they're both voiced by JenniferHale.
* TheSlacker: Wants to be famous without doing any of the work. She hires an intern to pretty much do everything for her in season 6
* SixthRanger: Yes, in one episode she wound up discovering the girls' secret and Jerry actually let her join WOOHP on the spot. Naturally, she wound up being a hinderence (though pivotal in stopping that episode's BigBad) that come the end of the mission, she ''begged'' to be released. To which she was mindwiped of the whole events.
* UngratefulBastard: She continues acting like a {{Jerkass}} to Sam, Clover, and Alex even after they saved her in an episode--although to be fair, Mandy usually gets her memory erased afterwards, so she doesn't actually remember Sam, Clover and Alex saving her.
* ValleyGirl: Even worse than Clover--and that's ''really'' saying something!

!!Arnold Jackson
-->'''Voiced by:''' America-Dee Baker, Joshua Seth; Canada-Rob Tinkler

* {{Adorkable}}
* NiceGuy

-->'''Voiced by:''' Darryl Kurylo

* TheAce
* ChickMagnet
* CurtainsMatchTheWindow

!!Dominique and Caitlin
-->'''Voiced by:''' Caitlin- Katie Griffin; Dominique- Andrea Baker

* AmbiguouslyBrown: Caitlin.
* {{Beta Bitch}}es: Dominique and Caitlin follow Mandy's lead.
* GirlPosse: To Mandy.
* [[SatelliteCharacter Satellite Characters]]: Other than being Mandy's friends and occasional rivals to Sam and Alex, respectively, Dominique and Caitlin don't really add much to the store. [[spoiler: They're written out of the series after ''Evil Graduation'' and are replaced by Mandy's cousin, Mindy, who's just like Mandy, except she's a DarkSkinnedBlonde with a thick Southern accent]].
* ThoseTwoGirls: They appear to be Mandy's closest, or other, her ''only friends'', because whenever Mandy's not with either one or both of them, she doesn't seem to hang out with anyone else--in fact, Dominique and Caitlin seem closer to each other than they do to Mandy.
* {{Valley Girl}}s

-->'''Voiced by:''' Katie Griffin

* AlphaBitch
* AmericanAccents: Speaks with a very strong southern accent.
* ChuckCunninghamSyndrome: Disappeared with no explanation after season 5.
* DarkSkinnedBlonde: Aside from having dark tanned skin and short blonde hair--and her accent--Mindy's essentially like a Mandy-clone.
* RedOniBlueOni: The much more calm and collected Blue Oni to Mandy's much more loud and aggressive Red Oni.
* SatelliteCharacter: Served as this to Mandy in season 5, replacing Dominique and Caitlin.