[[folder:Main Characters]]
!!!Peter Parker/Spider-Man
->Voiced by: Creator/NeilPatrickHarris

Bitten by a radioactive spider on a school field trip, young Peter Parker attempted to cash in on his new powers, and signed up for a pro wrestling contest, and pinned the champion. When the promoter cheated him out of his winnings, Peter stood by as a thief robbed the promoter.

The same thief later killed his Uncle. Peter began using his newfound powers responsibly and began fighting crime. One of the first criminals he faced was The Green Goblin. When The Goblin learned that it was Peter under the mask, he attacked his Aunt May, and kidnapped the girl he loved, Mary Jane. After a dramatic battle, The Green Goblin revealed himself to be Norman Osborn. Seconds later, The Goblin was killed when he accidentally impaled himself on his glider.

He later began studying at ESU, along with his dream girl Mary Jane and his best friend Harry, the son of The Green Goblin. Harry isnít aware that his Dad was The Green Goblin, and hates Spider-Man, believing he murdered him. Peter makes his living by selling pictures of Spider-Man to J. Jonah Jameson. After being ripped off one too many times, he decided to start selling video footage to Empire 1, where he met Indy, a research assistant who took a great shining to Peter.

Peter is still in love with Mary Jane, but feels that being with her would only jeopardize her safety again, which often leads to awkward feelings between the two of them, especially as he recently began seeing Indy [[spoiler: before he was tricked into throwing her off a building by the Gaines twins]].

[[spoiler: Vowing never to let Spider-Man hurt anyone else, Peter threw his Spider-Man costume into the river and stopped being Spider-Man]].

* AdaptationalAttractiveness: Looks NOTHING like the movie version of Peter the show is based on. In fact, this Peter shares an even stronger resemblance towards his comic book counterpart than Creator/TobeyMaguire does.
* BadassBookworm: He's intelligent and able to hold his own against all the criminals he fights.
* BewareTheNiceOnes: Kraven found out the hard way that it's a bad idea to set him off.
* ComesGreatResponsibility: Averted; This is the first adaptation do so but Peter never uses the trademark phrase nor does he ever refer to it.
* DeadpanSnarker: Wouldn't be Spider-Man if he didn't have a sardonic wit.
* ExpressiveMask: The eyes on his mask are able to register his emotions.
* GeniusBruiser: He has both brains and brawn.
* HeroesWantRedheads: As per usual (and just like in the films), Peter is very in love with Mary Jane (who has short, red hair).
* HeroWithBadPublicity: Among the worst he has ever received by the end of the series, where everyone in New York considers Spider-Man a menace in spite of all he's done for the city.
* LightningBruiser: He can hit hard and hit fast as well.
* UnstoppableRage: Unleashed upon Kraven after [[spoiler: the Gaines Twins create a mental illusion, causing him to think that Kraven killed Mary Jane]].
* YouFightLikeACow: It wouldn't be Spider-Man if he didn't taunt his enemies while fighting them.


!!!Mary Jane Watson
->Voiced by: Lisa Loeb

Mary Jane has been the object of Peterís desires since he was in the 2nd grade. After previously being engaged to Flash Thompson, she later moved onto Harry before she fell for Spider-Man, after he saved her life. Later realizing that she loved Peter, Peter rejected her love because he didnít want to cause her any further harm due to his double life as Spider-Man.

She later began majoring in drama at Empire State University, where she remained good friends with Peter and Harry. She still has feelings for both Peter and Spider-Man, not knowing they are really the same person.
* AdaptationalDyeJob: While her hair color is still red, The length of MJ's hair length here is pretty short compared to the traditional long and red hair that the Mary Jane of the comics (both Original and Modern) and other adaptations are usually depicted with having.
* BareYourMidriff: She wears a light purple long sleeved shirt that's cropped at her mid section.
* BettyAndVeronica: Competes with Indy for Peter's affections.
* DistressedDamsel: Though she ''is'' able to defend herself from time to time, and was the reason Spider-Man figured out destroying Shikata's sword would stop her. She even tried to do it herself.
* FieryRedhead: As per usual, she's a redhead who can get quite petulant. But, as said above, The length of MJ's hair is noticeably shorter than any other versions.
* GreenEyes
* HeroesWantRedheads: She has red hair and is Peter's main love interest.
* NotSoDifferent: After her ordeal with Christina, she suddenly realizes as she talks about it with Peter that Christina's whole "soul mates" obsession is too similar to her earlier attempts at forcing Peter to share intimate knowledge with one another.
* StoodUp: Well, she ''thinks'' this is what Peter has done to her several times in the series, but really he is prevented from turning up by unforeseen circumstances (battling villains, interrogated by the FBI, involved in a hostage situation, etc).


!!!Harry Osborn
->Voiced by: Ian Ziering

Never short of snappy one liners, Harry Osborn is the best friend of Peter Parker, the two of them live together off-campus and attend Empire State University together. Harry tends college for the social life, rather than the education, as he controls the majority of the stock from his late Fatherís company, Oscorp.

Despite being Peter and MJís best friends, he hates Spider-Man, blaming the wall crawler for his fatherís death, unaware that he was The Green Goblin. Still struggling to come to terms with his Dadís death, Harry is often found drinking or trying his luck with the ladies.

* AdaptationDyeJob: Creator/JamesFranco has reddish-brown hair in the role and most other versions of Harry are straight-up redheads. This Harry is blond.
* TheAlcoholic: Was heading toward having a drinking problem. Nearly every episode had him drinking something with alcohol in it.
* BrilliantButLazy: He's not dumb, but cares little about doing work, as evidenced by the quote:
--> Kids. Try to get them to spend their time wisely, like, watching R-Rated [=DVDs=]. But noooo... they can't wait to do their web research.
* DeadpanSnarker: Possibly more prone to making sarcastic quips than even Peter in this series. As opposed to his comic book incarnation.
* StrongFamilyResemblance: Looks quite similar to his father, Norman Osborn. Commented upon by MJ.



[[folder:Supporting Characters]]
!!!Indira "Indy" Daimonji
->Voiced by: Angelle Brooks

* BettyAndVeronica: Competes with Mary Jane over Peter's affections.
* CanonForeigner: She was created for the series in order to be an amalgamation of Gwen Stacy and Betty Brant.
* DeadpanSnarker: She has her moments of sardonic banter.
* GirlishPigtails: She has pigtails.
* {{Meganekko}}: A cute girl with glasses.
* StuffedInTheFridge: [[spoiler: Peter is tricked into pushing Indy off the roof of a building by the Gaines twins. The series ends with Indy in a coma, and her chances of awaking were slim to none. This is one of the factors in Peter giving up being Spider-Man.]]

!!!J. Jonah Jameson
->Voiced by: Keith Carradine

* AlliterativeName: His first, middle, and last name all begin with J.
* DaEditor: He's in charge of a newspaper and has quite a domineering attitude toward his employees.
* DeadpanSnarker: As opposed to his comic counterpart or other versions (who are usually portrayed as [[LargeHam Large Hams]])
* DemotedToExtra: He is nowhere near as prominent as his comic counterpart or other versions, only appearing in a small amount of episodes.

* AscendedExtra: Became Harry's girlfriend after being a background character throughout the series.
* CanonForeigner: She was created for this animated series and hasn't made an appearance in the comics.
* YouGottaHaveBlueHair: Her hair is pink.


[[folder: Villains]]

!!!Lewis Wyler/Turbo Jet
->Voiced by: Creator/HaroldPerrineau

* BitchInSheepsClothing: When he fights Spider-Man in public, he claims he's making a difference for the people. In private, he calls the very same people stupid for assuming he's a hero in the first place.
* CanonForeigner: He was created for the series.
* DirtyCoward: Seriously hurts a random bystander in his efforts to make an escape. He'd already done this to a security guard earlier putting him in a coma which was called out by Peter, but it was seen as a justifiable sacrifice by supporters (especially by Mary Jane who likened it to a policeman running over a bystander in a high speed chase) who believed he was a hero still trying to help the people at the time.
* {{Expy}}: He is loosely based on Rocket Racer, mainly in being an African-American villain with technology that gives him super speed.
* InsufferableGenius: He has a very low opinion of pretty much everybody around him.
* KickTheDog: The minute his facade gets blown, he tosses a decoy he was trying to steal at an innocent bystander and severely injures her. She can even be heard screaming and then sobbing in pain.
* StarterVillain: He is chronologically the first villain to be fought by Spider-Man in the series.
* VillainWithGoodPublicity: He becomes beloved by the people living in Villeroy Towers because circumstances led them to think he's helping them in their protests to keep their homes. In his last fight with Spider-Man, Spidey reveals to the protesters just how little Turbo Jet actually cares about them. In his frustration at being backed in a corner, he pretty much confirms what Spidey reveals.

!!!Wilson Fisk/The Kingpin
->Voiced by: Creator/MichaelClarkeDuncan

* BadassNormal: He has no powers, but is still a very formidable fighter.
* FatBastard: He's an obese criminal.
* GeniusBruiser: He's a criminal genius who is also very strong.
* LightningBruiser: He can move swiftly in addition to being strong.
* ManipulativeBastard: In his first appearance, he tricked Spider-Man into stealing a chip for him by pretending he was working for the government.
* RoleReprisal: The late Creator/MichaelClarkeDuncan reprises his role from ''Film/{{Daredevil}}'' movie as ComicBook/TheKingpin.
* ScaryBlackMan: He's an intimidating black criminal.
* VillainousGlutton: This version of Kingpin is shown to be obsessed with food. He threatens one of his underlings over the possibility of a french fry being missing in his bag of fast food and tops his cup of coffee with an assload of whipped cream. Spider-Man at one point even jokes about him eating his henchmen.

!!!Curt Connors/The Lizard
->Voiced by: Music/RobZombie

* AdaptationOriginConnection: In this version, he lost his arm to what's implied to be the prototype of what became the Green Goblin's pumpkin bombs.
* AdaptationalVillainy: Curt himself is nastier and more bitter than his usual portrayal, even pre-Lizard.
* BeastMan: Upon becoming The Lizard, he's a humanoid animal.
* DeathByAdaptation: He's alive and well in the comics, whereas here he ends up getting killed.
* FamilyUnfriendlyDeath: Got his arm cut off ''again'' by an air-conditioning exhaust fan, then fell from a helicopter. And his body is actually shown after the fall.
* HealingFactor: Similar to his mainstream counterpart, he can regrow body parts after losing them.
* KilledOffForReal: He is killed off permanently at the end of his debut episode.
* MadScientist: He's pretty focused on studying reptiles' regenerative abilities.
* PetTheDog: Before Curt became the Lizard, he was saved by Spider-Man during a robbery. As he's giving a statement to the police, he gets slightly pissed at the officer claiming Spider-Man and the robbers were working together even though he just said Spider-Man ''saved'' him.
* RoaringRampageOfRevenge: His arm was lost when Oscorp were testing a new explosive device in the same area as the swamp he was gathering samples from, known as [[FunWithAcronyms WAFER]] (Wide Area Fragmentation Explosive Round).
* WallCrawling: As the Lizard, he can scale walls.

->Voiced by: Creator/GinaGershon

* ActionGirl: She's a woman with great fighting ability.
* CanonForeigner: Another villain created for the cartoon.
* DecompositeCharacter: Despite being created for the series, She received the role of traveling from a foreign country into New York City in order to hunt and kill Spider-Man that is usually associated with Kraven the Hunter (who does show up later in the series).
* FaceDeathWithDignity: Starts looking very despondent when her sword is broken, but she recomposes herself and bows to Spider-Man, acknowledging his victory.
* HealingFactor: She can use her sword to heal wounds and gain youth by exposing it to moonlight.
* {{Hypocrite}}: She talks about honor, but is more than willing to use M.J. as leverage to finally get her fight with Spider-Man. Also, despite earlier making comments about not being scared by death, she visibly loses her cool the moment Spider-Man gets her sword (the thing that keeps her young). At the very least, when the sword gets broken, she bows in defeat before dying.
* KilledOffForReal: Rapidly ages and disperses into nothingness after Spider-Man destroys her katana.
* NobleDemon: Initially, she was hired by a rich collector to capture Spider-Man alive for him. After fighting Spider-Man for the first time and acknowledging him as a WorthyOpponent, however, she gives back the money to her employer after admitting she can't assure she'll not kill him, even mentioning it would be dishonorable to catch him for such a reason.
* NoImmortalInertia: She dies seconds after her sword is broken.
* ReallySevenHundredYearsOld: She used the magic of her katana to stay young for centuries.
* OffWithHisHead: She decapitates the guy who hired her to capture Spider-Man after she calls of their deal because he was interrupting things between her and Spidey.
* TheOnlyOneAllowedToDefeatYou: She cuts off one of her troops' trigger-fingers because he opened fire on Spider-Man, and ''no one'' decides how to take down an opponent but her.
* WorthyOpponent: Sees Spider-Man as an adversary worthy of her respect.

->Voiced by: Music/{{Eve}}

* BadassNormal: She's just a regular thief with no super powers, relying entirely on her gadgets to fight and perform heists. Despite this she's still able to match Spider-Man in fights and evade him.
* BitchInSheepsClothing: Subverted. Despite her antagonism with Spider-Man, her kindness towards Harry, Peter, and M.J. was genuine.
* CanonForeigner: She has no comic book counterpart, but is based on Black Cat.
* DatingCatwoman: Harry manages to fall for her civilian persona, Cheyanne, much to Peter's surprise. This is the reason why Spider-Man tries to get her to stop being a criminal.
* DontYouDarePityMe: She gets annoyed at Spider-Man's frequent attempts to figure out her FreudianExcuse and his constant pleading that she's better than this, demanding to know why he even gives a crap.
* {{Expy}}: She is loosely based on Black Cat and was even originally intended to be this continuity's version of Black Cat before the MTV executives persuaded the creators to retool the character to be more like her voice actress.
* ItsPersonal: She's a thief who is only interested in stealing objects that have emotional value to the people they belong to, saying if it's important to them that makes them worth stealing.
* SassyBlackWoman: She's a black woman who likes to mouth off others.
* SugarAndIcePersonality: A SpoiledSweet rich girl who Harry has serious feelings for and saves M.J. at one point, and also a cat burglar only interested in stealing things people would genuinely be despondent to lose and willing to manipulate the police into getting Spider-Man off her back.
* TakeThatKiss: Not a physical example, but she blows to kisses to Spider-Man to taunt him during their first fight. First, after she kicks him away and puts her goggles back on she turns back to him and blows a kiss before running off. Then, after Spider-Man is stuck holding a piece from a construction building in place to stop it from falling into the streets, Talon blows him another goodbye kiss to taunt him before dissappering.

->Voiced by: James Marsters (Sergei)

* ButtMonkey: In their encounters with Spider-Man, Alexei was usually the first guy who wound up in webs.
* CanonForeigner: Villains exclusive to this continuity who never appeared in the comics.
* {{Jetpack}}: They all wear jetpacks so they can fly.

->Voiced by: Creator/TaraStrong

* AntiVillain: Due to the fact that she received actual brain damage. She's nuts and she did try to kill M.J., but she legitimately believes that it's what Spider-Man wants because her mind is telling her that. Christina needs medical attention and a good psychiatrist.
* AxCrazy: It sort of helps that her psychosis manifested as a result of actual brain damage thanks to her malfunctioning ESP crown. That being said, she takes M.J. hostage with her acid gun and it makes it clear she'll use it on her, laughing at the idea of her organs splayed out on the ground in a puddle.
* CanonForeigner: Yet another villain who was created exclusively for this continuity.
* {{Cloudcuckoolander}}: One has to wonder how she came to the conclusion that a stout coffee vendor was Spider-Man's true identity.
* EyeTwitch: During her last attempt on M.J.'s life.
* IJustWantToBeLoved: She only wants someone to love her.
* KilledOffForReal: Averted. Despite being trapped in an elevator as it plummeted to the ground, she just ended up on a stretcher with a neck brace.
* LoonyFan: Oh so very much. She believed she was soul-mates with Spider-Man long before she got zapped.
* LoveMakesYouEvil: As long as she can get Spider-Man, she doesn't seem to think there's anything wrong in committing murder to get him despite one of the things she finds attractive about him is his heroism.
* LovingAShadow: She loves Spider-Man, but more precisely she loves the image of him and the hallucinations her brain is creating. Christina thinks the only reason the real Spider-Man isn't with her now is because he doesn't know he loves her and needs coaxing.
* MadScientist: She built a glue gun in high school that turned out to be highly acidic and dangerous, and her ESP crown actually does let her read minds. The wiring is a mess, though.
* MotorMouth: She talks very rapidly.
* MulticoloredHair: Her hair is black with streaks of orange, yellow, and green.
* {{Nosebleed}}: Whenever she hallucinates or whenever she is acting particularly psychotic her nose will begin to bleed, most likely an indicator of her brain damage.
* PerkyGoth: She has black lipstick and dark hair, yet she's very excitable and claims she can get along with almost anyone.
* PsychopathicManchild: As she has M.J. hostage, she indicates being insane and childish:
-->'''Mary Jane''' (''Scared''): How do you know it's you Spider-Man wants? How do you know it's not someone else?
-->'''Christina''' (''Laughing and holding her acid gun to M.J.'s face''): Then ya better hope it's not ''you!''
* RescueRomance: Tries to pull this off. She expects Spider-Man will save her when she walks off the roof of a ''very'' tall building, and he does. But the "romance" part doesn't take off. She gets sad and angry later on because she was "Just trying to be a good girlfriend."
* SanitySlippage: Despite her StalkerShrine, she didn't become dangerous until after her ESP crown electrocuted her. Instead of giving her powers, it made her hallucinate that her pictures of Spider-Man were talking to her and ordering her to do whatever it took to make sure they became a couple, including kill Mary Jane.
* SlasherSmile: Has a creepy grin, especially after she goes after M.J..
* StalkerShrine: Naturally she has one to Spider-Man in her closet.
* StalkerWithACrush: She has an obsession with Spider-Man and desperately wants to be with him.
* ThatLiarLies: When M.J. asserts she's not interested in Spider-Man. "You LIE! I don't like liars!"
* WrenchWench: Not so much for cars, but she has jury rigged an acid gun, and an ESP crown, although the latter has some issues...
* {{Yandere}}: She flips out when faced with the truth that Spidey isn't interested.

!!!Max Dillon/Electro
->Voiced by: Ethan Embry

* AxCrazy: As Electro, he becomes insane and violent.
* BecauseYouWereNiceToMe: Why he tries to convert Sally into an electrical being like him, thinking she actually loves him.
* CompanionCube: Ditches Peter for a partner in a class project, for a AI computer version of himself.
* DisproportionateRetribution: ''KILLED'' a college senior because he was constantly pranking him. To be fair, one of those pranks nearly got him DROWNED IN CEMENT!
* EarlyBirdCameo: Appears as a minor character in "Head Over Heels" before his role is expanded on and he becomes Electro in "The Party".
* FaceHeelTurn: He started off as one of Peter's friends from high school and didn't turn into a villain until halfway into the show.
* IJustWantToHaveFriends: He just wishes for acceptance among his peers.
* KilledOffForReal: His second appearance as Electro ended with him being destroyed by getting sucked into an [=OsCorp=] containment unit and tossed into the sea.
* OneWingedAngel: When drained of electricity, he turns into a thin, clawed version of himself
* PsychoElectro: As Electro, he has electrical powers and is mentally unstable.
* ShockandAwe: How he received his powers, just like in the comics.
* WoobieDestroyerOfWorlds: He has some issues, yes, but it's hard not to feel bad for the guy in spite of what he does.

!!!Dr. Zellner
->Voiced by: Creator/JeffreyCombs

* CanonForeigner: Created exclusively for this continuity.
* {{Expy}}: He reminds many fans of Miles Warren, the mastermind of the ''[[ComicBook/TheCloneSaga The Clone Saga]]''.
* MadScientist: He has very few scruples and has created a smart drug with potentially fatal side effects.

!!!The Biker Twins/Jack and Mack
->Voiced by: David [=DeLuise=]

* AllThereInTheScript: Their names are only given in concept art and model layouts, which are visible as special features on the show's DVD.
* CanonForeigner: Created exclusively for this continuity.
* DumbMuscle: They're both thugs who aren't very smart.
* IdenticalTwinIDTag: Jack is bald and wears yellow, while Mack has a beard and wears red.
* ThoseTwoBadGuys: They're never seen apart.

->Voiced by: Virginia Madsen

* BadassNormal: She has no powers, but is still a formidable adversary to Spider-Man.
* EnemyMine: Works together with Kraven, a guy she would usually kill, to get vengeance on Spider-Man. [[spoiler:But that's just an illusion.]]
* HeroAntagonist: Though she was attempting to assassinate an important politician, she had really good reasons to do so; the man was actually a terrorist attempting to infiltrate the White House.
* KnightTemplar: Her mission had a honorable goal, but she was willing to use methods such as stealing weapons, taking teenagers hostages (and wanting to kill them herself when it didn't work as planned), and putting a bomb on the mayor's car.
* NeverFoundTheBody: She apparently ends up killed, but no corpse is found.

!!!Kraven The Hunter/Sergei Kravenoff
->Voiced by: Creator/MichaelDorn

* BadassBaritone: Due to having Creator/MichaelDorn as his voice actor.
* BadassMustache: Per usual, he has an impressive moustache.
* BadassNormal: As per usual, Kraven is a skilled hunter whom Spider-Man defeated prior to the series and he does not appear to have any superhuman abilities.
* BoisterousBruiser/BloodKnight: Zigzagged with both.
* DecompositeCharacter: The role of traveling from a foreign country into New York City in order to hunt and kill Spider-Man (due to being contacted by a criminal mastermind that hates the Web-Slinger and wants him dead) that is usually associated with the Kraven of the comics and most versions is given to CanonForeigner Shikata.
** Although, the Kraven of this series still portrayed a skilled hunter from Russia whom Spider-Man has encountered prior to the series.
* EgomaniacHunter: He was willing and able to hunt and kill anything (particularly the parents of the Gaines Twins) until he fought Spider-Man.
* EnemyMine: He works together with Silver Sable in order to get vengeance on Spider-Man. [[spoiler:But it's just an illusion.]]
* HuskyRusskie: His Russian accent is not as emphasized as the other versions, but it's still there.
* KilledOffForReal: Subverted; He was about to be this during a battle that he fought with Spider-Man in an empty power plant when the Web Slinger was about kill him (through nearly hanging Kraven! Now that's harsh!) after [[spoiler: the Gaines Twins created a mental illusion that caused Spider-Man to believe that Kraven killed Mary Jane]]. But fortunately for Kraven, Spidey snaps out of it (after he saw outside the ESU science sign when he remembered it was ripped off), relents his decision, and turns Kraven over to the police.
* MovieSuperheroesWearBlack: An animation (and super villain) example. This Kraven wears a black leather jacket instead of the lions mane vest of the comics and most adaptations.

!!!The Gaines Twins/Roland and Roxanne
->Voiced by: Kathy Griffin (Roxanne Gaines), Jeremy Piven (Roland Gaines)

* CanonForeigner: They were created exclusively for this continuity.
* CreepyTwins: A pair of eerie twins with mind control powers.
* KilledOffForReal: They end up apparently dying in an explosion.
* {{Manipulative Bastard}}s: They use their mind powers to manipulate and deceive people.
* MindControl: They have the power to influence people's minds to manipulate them and make them see what isn't really there.
* NonActionGuy: Roland. When he makes the notion that they should be trying to kill Kraven themselves, rather than mind-controlling Spider-Man into doing it, Roxanne points out that his senses are too keen in order for them to get close enough and brings up a NoodleIncident of "the last time you [Roland] used your tiny little hands to fight." Later when he switches from using his mental powers to attempting to attack Spidey with a pipe, he does indeed get his ass kicked.
* ThemeTwinNaming: Their names both begin with R.
* YouKilledMyFather: Kraven the Hunter captured their parents for scientific experimentation, which eventually killed them, but it also gave Roland and Roxanne their mental abilities. Rather than try and kill him themselves however, they opt for Spider-Man to do it by manipulating him.