!!Hazumu Osaragi
!!!'''Voiced by:''' Creator/KanaUeda (Japanese), Creator/StephanieSheh (English)
-->''"Okay. Starting tomorrow I'll try again! I'll become a new me..."''

* AttractiveBentGender: After being turned into a girl, (s)he has been given a ''very'' nice body.
* BlindingBangs: As a boy.
* ChildhoodFriendRomance: With Tomari.
* DudeLooksLikeALady: Yasuna accidentally mistook Hazumu for a girl when s/he was still a "he".
* FirstEpisodeResurrection: Was killed when (s)he was hit by Hitoshi's spaceship. (S)he was brought back to life afterwards, but at the cost of irreversibly switching his gender to female.
* GenderBender: After being killed by a falling UFO, the aliens responsible for the crash revive Hazumu as a girl. However, Hazumu doesn't make a whole lot of fuss about it, and it's implied that [[{{Transgender}} she's actually happier as a girl than she was as a boy]].
* InnocentFanserviceGirl: Justified as s/he just turned into a girl and is therefore unfamiliar with how s/he should act, such as using her skirt to cool his/her legs, and asking Asuta to measure his/her bust size.
* MsFanservice
* NakedOnRevival: Averted in the manga, but played straight in the anime when the now-female Hazumu is revived after being crushed by Jan-Puu's spaceship form.
* NicePerson
* {{Transgender}}: It's strongly implied that Hazumu was a trans girl all along without realizing it, and that may have been the reason behind the GenderBender in the first place. She doesn't protest at all about becoming female and seems to be much happier as a girl than she was as a boy.

!!Yasuna Kamiizumi
!!!Voiced by: Creator/YuiHorie (Japanese), Creator/KarenStrassman (English)
-->''"I don't dislike you, Hazumu kun... if... if... if you were a girl..."''

* BettyAndVeronica: The Betty to Tomari's Veronica.
* DoesNotLikeMen: Subverted. She just can't see them. Even her father, who she loves dearly.
* NiceGirl
* ShrinkingViolet

!!Tomari Kurusu
!!!Voiced by: Creator/YukariTamura (Japanese), Creator/MonaMarshall (English)

* BettyAndVeronica: The Veronica to Yasuna's Betty.
* ChildhoodFriendRomance: With Hazumu.
* FirstGirlWins: Played straight in the manga. Double subverted in the anime as initially Hazumu chooses Yasuna, however an OVA was released post-series that showed that the two later broke up.
* GirlishPigtails: She wears her hair in pigtails.
* IfItsYouItsOkay
* MatchmakerCrush: She pushes Hazumu to confess to Yasuna, but then increasingly owns up to her own feelings after his genderswap.
* RapunzelHair: Her hair is very long, especially when not in pigtails.
* {{Tomboy}}
* {{Tsundere}}: Type A.

!!Ayuki Mari
!!!Voiced by: Creator/MasumiAsano (Japanese), Camilla Derby (English)

* {{Asexuality}}: Is heavily implied to be an aromantic asexual.
* CelibateHero: She has no interest in romance.
* DeadpanSnarker
* {{Meganekko}}
* ReiAyanamiExpy: She has the look.
* YouGottaHaveBlueHair

!!Asuta Soro
!!!'''Voiced by:''' Creator/DaisukeOno (Japanese), Creator/DougErholtz (English)

* AllLoveIsUnrequited: To female Hazumu. He's pretty {{Squick}}ed out about it.
* ButtMonkey
* TheOneGuy: The most prominent male character of the main cast.
* UnrequitedOrientation: Hazumu isn't interested in boys.