A list of characters from the animated movie Justice League vs. The Fatal Five.
Characters listed with an asterisk (*) are cameos only.
- Batman: The Animated Series
- The Bat-Family: Robin II (Tim Drake)*
- Allies: Zatanna*
- Rogues Gallery, Part 1: Harley Quinn, Two-Face, Poison Ivy
- Superman: The Animated Series:
- Justice League
- The Justice League: Green Lantern (John Stewart)*, Hawkgirl*, Martian Manhunter*, Green Arrow*, Black Canary*, Captain Atom*, Vixen*, Mister Terrific, Green Lantern/Limelight (Jessica Cruz), Miss Martian, Green Lantern (Hal Jordan)*, Green Lantern (Guy Gardner)*
- Allies & Friends of the Justice League: Captain Marvel*, Huntress*, Kilowog
- Other Villains: The Fatal Five (Emerald Empress, Mano, Tharok, the Persuader, Validus), Bloodsport