!!!'''Pearl Jones'''
A young girl living in LA during the 1920s with dreams of stardom... until she's invited to the wrong party.

* ActionGirl: After being converted into a vampire.
* BattleCouple: With Henry.
* EeriePaleSkinnedBrunette
* OurVampiresAreDifferent: The american vampire has elongated finger-claws, rattlesnake fangs, and are powered by sunlight, only becoming vulnerable during the nights of the new moon and can only be killed by gold.
* TookALevelInBadass: Pearl after becoming a vampire.
* UnstoppableRage: Pearl. After the Hollywood Coven does bad things to her, her rage is only stopped when they're corpses.
* ViolentlyProtectiveGirlfriend: While Henry's usually able to handle himself, if he's in over his head, particularly against vampires, Pearl's right there and ready to rumble.

!!!'''Skinner Sweet'''
A psychotic outlaw in the Wild West who runs afoul of the Pinkerton Detective Agency and a group of European vampires. Becomes the first American Vampire.

* AxCrazy: Whenever he shows up, there's a very good chance a lot of people are going to end up dead.
* [[spoiler:BackFromTheDead: As of #23]]
* BuriedAlive: For 20 years.
* ChronicBackstabbingDisorder: If you ally with him... watch your back.
* EvilFormerFriend: Formerly Jim Book's best friend and adopted brother.
* FauxAffablyEvil: Sure he seems like a friendly guy, but don't let that fool you. He'll rip you to shreds without a moment's hesitation, just for a laugh.
* FirstOfItsKind: The first American Vampire.
* ManipulativeBastard
* OurVampiresAreDifferent: The american vampire has elongated finger-claws, rattlesnake fangs, and are powered by sunlight, only becoming vulnerable during the nights of the new moon and can only be killed by gold.
* PetTheDog: Skinner saving Pearl's life may count (though it is possible he has ulterior motives or simply wanted to screw up the Hollywood Coven's plans).
** Skinner's interaction with Kitty at the ending of 'Strange Frontiers'.
* SlasherSmile: Seems to be his preferred expression.
* SweetTooth: He retains this trait even after becoming a vampire.
* WorthyOpponent: Skinner sees Jim Book as this, as seen in 'Strange Frontiers' when he murders the actor who is playing the part of Book in a Wild West show.
** ''You don't deserve to wear Jim Book's name!'' *tears off head*
* TheWildWest

!!!'''Henry Preston'''
A Marine who served in World War I before becoming a traveling musician who crosses paths with Pearl in Los Angeles and falls in love with her.

* BattleCouple: With Pearl.

!!!'''Hattie Hargrove'''
Pearl's best friend and roommate, also an aspiring actress.

* AxCrazy: [[spoiler:When she comes back from her near-death experience, she becomes much more wantonly murderous and bloodthirsty.]]
* EvilFormerFriend: Goes from Pearl's best friends to one of her greatest enemies.
* EvilIsPetty: Set her best friend up to be vampire-chow just so she can become a famous actress.
* EvilRedhead: An AxCrazy vampire with bright orange hair.
* GlasgowGrin: As the result of having the gold star from her dressing room shoved down her throat.
* IHaveYourWife: Pearl is lured into an ambush when Hattie gets kidnapped by vampires. Unfortunately for Pearl, Hattie is in on the whole thing.
* NotQuiteDead: [[spoiler:Pearl appears to kill her near the end of volume one, only for it to eventually be revealed that she was merely put into a death-like state. Other vampires recovered her body from the morgue and used her as a guinea pig to find out the weakness of American Vampires.]]

!!!'''James Book'''
A veteran of the Civil War, he becomes a detective in the Pinkerton Detective Agency and hunts down Skinner Sweet.

* ICannotSelfTerminate: [[spoiler:Asks his best friend's daughter to kill him rather than live as a vampire.]]
* MayDecemberRomance: His best friend's daughter has a crush on him despite possibly having at least 20 to 40 years on her. [[spoiler: Taken to an extreme degree when she only agrees to kill him if he slept with her and gave her a child to remember him by.]]
* PinkertonDetective: A rare example of one played purely heroically.
* TragicMonster: [[spoiler:Gets turned into an American Vampire when Skinner Sweet's blood enters his body.]]

!!!'''Felicia Book'''
Jim Book's daughter (born after [[spoiler:his death]]). Is seeking vengeance on Skinner Sweet and becomes an agent of the Vassals of the Morning Star.

!!!'''Cash [=McCogan=]'''
The police chief of Las Vegas in 1935 who is drawn into a string of mysterious deaths that bring him into the world of vampires.

!!!'''Linden Hobbes'''
A mysterious agent who is the leader of the Vassals of the Morning Star.

* CoolOldGuy
* [[spoiler: KilledOffForReal: In an appropriately badass way, naturally, given that he takes DRACULA with him.]]
* KnightTemplar: Surprisingly averted. In his first appearance, he seems to be obsessed with destroying all vampires, no matter how harmless, however, by the 'Lord of Nightmares' arc, he's mellowed out a lot, allying himself with Pearl and Felicia, two known vampires, as well as allowing The Firsts, a group of surviving vampires who are out to kill Dracula to live.
* ReasonableAuthorityFigure

!!!'''Travis Kidd'''
A young, rebellious vampire hunter in the 1950s who is... extremely talented at killing vampires.

* BadassNormal: Possibly the series's greatest example, lacking any powers, allies or decades of expierence, but still taking on scores of vampires on his own and fighting Skinner Sweet to a standstill.
* GreaserDelinquents