Shinji calling his father out twice. The first of them when his father planned goading him into piloting Unit-01 for -what Gendo believed- first time. And the second when Shinji walked into his office and communicated him what he was piloting ON HIS TERMS, and he also demanded a prize for risking his neck (all the while trying not showing he was still frightened of his father).
Asuka and Shinji fighting Gaghiel in Unit-02. They are losing because Shinji can not focusing properly, when he thinks of reminding Kyoko her daughter is about of dying. Instantly Unit-02 goes berserker and easily opens Gaghiel's jaws. Asuka and Unit-02 reached perfect synchronization right then.