History Main / TertiarySexualCharacteristics

* In ''Anime/{{Berserk}}'' Casca despite managing a SweetPollyOliver with her amour still wears a pink tunic in the anime, skin tight legging making her buttocks pronounced and even thigh-high boots in contrast to male of the Band of Hawk.

* In the spinoff ''WesternAnimation/PussInBoots'' the main cue that Kitty Softpaws is female is that she has long eyelashes, which don't appear till [[spoiler:after she [[SamusIsAGirl removes her luchadore mask]]]] and stay around when [[spoiler:she puts the mask back on]], maybe Puss was just being unobservant that day?

* This may have been an attempt to get around the all-too-common ReptilesAreAbhorrent effect, but Master Viper in ''WesternAnimation/KungFuPanda'' has eyelashes, flowers on her head, and slightly reddish lips; pretty much the opposite of Master Tigress (see below), who is androgynous to the extent that [[ViewerGenderConfusion many people]] (including her own [[Creator/AngelinaJolie voice actress]]) initially thought she was a guy. This also suggests that it may be a deliberate choice on the part of the ''character'', who seemed a bit more secure in both her femininity and her martial prowess than the [[ProudWarriorRaceGuy Proud Warrior Woman]] Tiger. It could also be because that's all she could be wearing and still believably move like a (cartoon) snake; it's harder to pull off CivilizedAnimal without copying either clothing or human movement.
* In early Russian StopMotion classic ''Animation/TheCameramansRevenge'', a (presumably) sexy nightclub dancer is represented by a dragonfly with long, delicate wings.
* Female animals in the ''WesternAnimation/IceAge'' movies tend to have longer eyelashes, lighter colored fur and, when applicable, obviously feminine bodies. Female sloths in particular have girly "hair" and what appears to be ''lipstick''. In the audio commentary for ''The Meltdown'', it is joked by the character designers that they would have saved themselves a lot of trouble if they'd just stuck a bow on Ellie's head rather than try to modify Manny's design to make it feminine. ''Dawn of the Dinosaurs'' features Scrat facing off against a female Scrat for an acorn. Scratte has dark foxy coloring, a significantly curvier body, eyelashes, and ''blue eyeshadow''. She may possibly also be a separate species since, like [[BizarreSexualDimorphism female]] [[Film/TheDarkCrystal gelflings]], she can glide.
* Very subtly done in ''WesternAnimation/HappyFeet''. The female emperor penguins have purple undersides under their beaks and males have orange. The females also have their chest feathers shaped to vaguely resemble breasts.
* ''WesternAnimation/{{Smallfoot}}'': Female yetis have thicker fur around their legs, suggesting that they are wearing skirts.
* Moxy, the lead character in ''WesternAnimation/{{Uglydolls}}'' is pink with hair-like extensions and thick eyelashes. She is the only Uglydoll in the movie to have the latter.
* Inverted with Littlefoot in ''WesternAnimation/TheLandBeforeTime''. A notable part of his design is his long, feminine eyelashes, despite being a boy.

* Ojo from ''Series/BearInTheBigBlueHouse'' is female, but lacks any human hair, eyelashes or makeup, wears no clothing or accessories, has a TomboyishVoice (keep in mind that prepubescent boy characters tend to be voiced by women) and her fur is a darker shade of orange than Bear's, inverting PaleFemalesDarkMales. Even her name sounds like a boy's name. It's enough to cause some ViewerGenderConfusion.

* Creator/ArinaTanemura depicts her characters with overt sexual dimorphism, quite typically like many other manga creators: guys tend be taller, have noticeable sternocleidomastoid muscles (SCM), smaller eyes which are mostly [[TsurimeEyes tsurime]]; while girls tend to be shorter, have no noticeable SCM, much bigger and childlike eyes which could be either [[TaremeEyes tareme]] or tsurime (although being girls, their tsurime look [[MasculineLinesFeminineCurves softer and less sharp]]), and of course, absurdly long and luscious eyelashes. What's notable about Tanemura's style is that she has designed transvestite characters on more than one occasion (examples include Maora from ''Manga/TheGentlemensAlliance'' and Maimai from ''Manga/SakuraHimeKaden''), in which a male character, when donning a feminine outfit, barely resembles his male self at all: his physical appearance (all of those traits mentioned above) completely changes accordingly to his so-called "[[DisguisedInDrag female disguise]]." That is to say, thanks to some weird rules in the Tanemura multiverse, a tall and hunky guy can completely turn into a little petite girl merely by putting on a skirt, some makeup and girly accessories. These characters tend to serve as mere plot twists that will put even the most adamant suspensions of disbelief to shame.

*** As far as secondary characteristics go, males lack eyelashes and have more angular muzzles and narrower ears than females. Some are noticeably taller and stockier, but not all; for example, the exposed muzzle is the only way to determine Wonderbolt (and Shadowbolt) genders (unless they [[BadassMustache really want to let you know]]). There are, however, a couple of mixed and non-standard character designs in the background cast, which have been cause for endless confusion. Also, most male ponies [[note]] usually generic background ones, which in turn was one of the major inspirations for the fandom creation of Doctor Whooves [[/note]] tend to have ruffled or spiky hair while almost all female ponies' hair are completely smooth and straight. In one episode, Rarity was shown to wear false eyelashes, and she's been seen applying mascara to Fluttershy. The main difference seems to be the size of the male ponies who are always one-half or one head taller than all the females (with the obvious exception of [[LargeAndInCharge the Princesses]], of course). For ponies, who are around three heads tall, this is actually quite a large difference.

* Whenever you perform a task in your schedule, a simplified version of your avatar is shown doing it. Female avatars will wear a ponytail [[GameplayAndStorySegregation regardless of their hairstyle.]]

* Ms. VideoGame/PacMan has a bow on her head. In the AnimatedAdaptation, Sue, the DistaffCounterpart of Clyde, became a purple ghost with eyelashes and earrings.
** Ms. Pac Man also has an eyelash, and a mole, and if you look real closely, you can also see lipstick.
** Discussed in an exchange in ''Film/WaynesWorld'', which provided this trope's alternate title:
-->"Well, she's got a bow on her head!"
** The minuscule difference between Pac-Man and Ms. Pac-Man was played with in WesternAnimation/DrawnTogether, where Pac-Man reveals that he ''is'' Ms. Pac-Man when he puts on the bow.
** One of the ads for a home video game system version of Ms. Pac-Man had the titular yellow gobbling-disk try to convince us otherwise, by singing "Honey, don'tcha know / I'm more than Pac Man with a bow!"
** The cartoon version of Pac-Man at least got an article of clothing like his wife: he's almost never seen without his trusty red hat.
* The female PlayerCharacter in ''VideoGame/ThePedestrian'' has the lower half of her body be composed mainly of a triangle with legs coming out the bottom. This triangle is meant to be a dress.

* ''VideoGame/KingdomOfLoathing'': Female avatars have ponytails. Many of the alternate avatar images, as well. The male version of Vestments of the Treeslayer is a tree. The female version is the same tree, with a little bow tied to one branch.
* ''Franchise/MassEffect'':
** The only female krogan seen wears a full body-covering robe, while males are almost always wearing combat armor, and the few who don't wear simple shirts and trousers. However, they still were able to split off from the males and form their own exclusively female clans that are doing just fine on their native DeathWorld Tuchanka without any males protecting them from wild beasts and raiders, so it's more likely that the one example of female clothing were special priestess robes rather than common clothing for women. [[spoiler:And even then she still had no problem at all with taking a shotgun from the person next to her to shoot a cyborg assassin coming up behind him.]]
** Quarian females generally wear a hood or veil over their helmet, while males have little hook things hanging from their rebreathers. These are on top of form-fitting full-body environment suits that clearly show very prominent humanoid sexual dimorphism [[note]] Female: wide hips, Male: wide shoulders and such. All Quarians we've met are quite slim which accentuates both. [[/note]], moving away from the trope somewhat.

* The ''VideoGame/PaperMario'' games do this quite a bit:
** Generic Toad [=NPCs=] are identified as female by having long hair and eyelashes, which is a little strange, given the ViewerGenderConfusion suffered by the original Toad over the years.
** Bombette, a female Bob-Omb, is pink, has a blond, braided fuse, and a heart-shaped windup key.
** Goombella is also pink, and she has a blond ponytail.
** Goombario wears a blue cap similar to Mario's, while his sister Goombaria wears an orange-red bow. And has a pink body. Their mother is also pinkish in color and wears a pink head kerchief, although their grandmother is brown and merely has a lacy cap. However, none of his three female relatives have hair, unlike Goombella.
** Ms. Mowz, a Mouser, has a heart-shaped tail and wears high heels.
** Interestingly, Watt and Sushie are aversions. Sushie otherwise looks like an ordinary Cheep-Cheep except for purple and orange coloring, and Watt, a Sparky, has only a pacifier (to signify that she's young). There's still some [[ViewerGenderConfusion confusion]] among gamers regarding Watt's gender. The fact that the game refers to Watt as both male AND female at different points doesn't help either.
** Petuni, the only known female Puni (other than the Elder), can me distinguished by the ''pink'' orb on her antenna.
** Koops's girlfriend is a Koopa Troopa with eyelashes, hair, and BlushStickers and is the only female Koopa Troopa in the game.

* Averted in ''VideoGame/SuperMarioRPG''. At one point Mario and company encounter a Chain Chomp visually identical to every other Chain Chomp in the game. Bowser however is easily able to determine that it is a girl and then "acquires" her as a weapon that he can hurl at enemies.
* ''Franchise/SuperMarioBros'':
** Wendy O. Koopa has a pink hairbow, a pink shell, makeup, jewelry and high heels. Interestingly, despite being the only female Koopaling, Wendy is one of only two Koopalings with no hair.
** Pom Pom, a feminized version of Boom Boom. Even her ''boomerangs'' have bows on them.
** A recurring quest in ''VideoGame/SuperMarioOdyssey'' requires the player to capture a Goomba and meet up with another, female Goomba somewhere in the kingdom. You can tell she's a girl because she has long eyelashes, {{blush sticker}}s, and--oh yeah--is pink. (Actually, she looks a lot like [[VideoGame/PaperMarioTheThousandYearDoor Goombella]].) Atypically for this trope, though, she also wears a red cowboy hat.
* ''VideoGame/ChronoTrigger'': Atropos, Robo's DistaffCounterpart, DuelBoss, and girlfriend is pink and wear a bow. In fact, after you beat her in her DuelBoss fight versus Robo, she gives you her ribbon as a special Robo-only equippable item.
* In ''VideoGame/TheSims2'', you can build male and female Servos (robot butlers). The male looks like a generic robot, but the female looks like the male with a pink bow and lipstick.
* ''VideoGame/MySims'':
** Sims are technically neither, though the specific characteristics they're given tell the tale. The player-controlled Sim can be given any available characteristics you want. (Feminine voice, eyelashes, and attire with a masculine hairstyle and a five-o'-clock shadow? Go for it!)
** In ''[=MySims=] Kingdom'', on the other hand, you choose a gender for your Sim, and that puts a limiter on what your Sim can wear, what kind of hair is available, etc.. Sometimes, it changes what outfits you get as a reward. For example, there's a reward that lets males choose from a selection of outfits that are shorts-only; females get two-pieces, some with shirts over them (split near the bottom).
* ''Franchise/{{Pokemon}}'': Gender-specific physical differences were created for some Pokémon in ''VideoGame/PokemonDiamondAndPearl'' and carried over to future games. Most of them, as well as earlier attempts at portraying sexual dimorphism in the franchise, fall under SecondarySexualCharacteristics, being random changes or based on real life animals, but a few, such as female Wobuffet's lipstick-like markings, are closer to this, via RuleOfFunny.
* ''Website/{{Neopets}}'' didn't always have gender differences, but after the pet artwork received a global update, female pets gained conspicuously longer eyelashes over their male counterparts -- or, in the case of the insect-like species: partially-closed eyes.
* When Amy Rose first appeared in ''VideoGame/SonicTheHedgehogCD'', her defining sexual characteristics are being pink (a contrast to Sonic's blue), eyelashes, and a skirt. When she was redesigned for ''VideoGame/SonicAdventure'', her femininity was more emphasized by changing her hairstyle (up until then, it was exactly the same as Sonic's), giving her an actual bust size (she was changed from eight years old to twelve), and giving her large golden bracelets to wear on her wrists. [[ComicBook/SonicTheHedgehogArchieComics Archie]] gave her secondary characteristics. Played straight with most characters in the games, where the only differences are eyelashes and clothing styles.
* Becoming a Super Mutant in ''VideoGame/{{Fallout}}'' causes one to lose secondary sexual characteristics and become sterile, muscle-bound, and masculine-looking. Two female Nightkin in ''VideoGame/FalloutNewVegas'' accessorize to look feminine: Lily wears a gardening hat and shawl, while Tabitha sports a pair of heart-shaped red eyeglasses and a blonde wig with a bow.

* In ''Nuts & Milk'', Yogurt looks exactly like Milk with the addition of two pink bows on her head.
* In ''VideoGame/IceClimber'', Nana (the P2 character) is just the Popo sprite with a pink parka instead of blue.
* In ''VideoGame/BanjoKazooie: Nuts and Bolts'', Rare apparently got tired of the years of people going [[ViewerGenderConfusion "Kazooie's a girl?!"]], so they re-made her appearance. She now has long eyelashes, "curly" hair, and a more curvy look, to make her more obviously female. And then, once you beat the game, she starts wearing a bowtie (yes, it's a bow, but it's a masculine bow). Kazooie even keeps her Eyelashes in Super Smahs Bros. Ultimate to show that she is undoubtedly a Girl.
* The protagonist of ''VideoGame/IWannaBeTheGuy'', The Kid, gets a bow in his hair on Normal Mode, to [[EasyModeMockery suggest the less than masculine choice of playing on the easiest setting]] As if the extra save points with "WUSS" written on them weren't enough of a clue. Ayane gives Ryu one in the Xbox ''VideoGame/NinjaGaiden'' if he selects Ninja Dog mode.
* ''Franchise/{{Kirby}}'':
** All over the place in the ''Kirby'' series, where characters are given bows to show that they're female. It's most obvious with Chuchu the octopus and Ribbon the fairy. The only two exceptions seem to be [[CuteWitch Keke]] and Adeleine. This caries over into the fandom, where fanmade female characters are usually given a bow, or at least big ol' eyelashes.
** The blue Lololo's distaff counterpart Lalala is pink and wears a bow.
** Taken to the extremes with Kaboola/Kabula, an airship boss in ''VideoGame/KirbysDreamLand'' and ''VideoGame/KirbySuperStar Ultra''. Her most recent redesign has eyelashes painted on her hull.
** Many male characters in this series tend to have big bushy eyebrows.
** Inverted in ''VideoGame/KirbysEpicYarn'' with an angler fish couple Kirby is responsible for reuniting, as the male is smaller, pink and wears a bow around its body.
* ''VideoGame/BubbleBobble'':
** The protagonists of ''Bubble Symphony'', a sequel to ''Bubble Bobble'': Coro and Kulu, who have bows on their heads, are the female duo BetaCouple to Bub and Bob.
** Pab and Peb, the new Beta Couple in ''Bubble Bobble Plus (UsefulNotes/WiiWare)''. (See the Title Operations Guide in the Wii Shop Channel.)
** ''Rainbow Islands'': [[spoiler:Three out of eleven random people who have all been imprisoned in TheAlcatraz and transformed into green bubble dragons are female and thus have a pink bow on their head.]]

* In ''VideoGame/FinalFantasyIX'', female Moogles are distinguished from the males by wearing pink vests.
* In one quest from ''VideoGame/FinalFantasyTacticsA2'' you rescue a kidnapped Seeq beauty queen. Her sprite is indistinguishable from a male Seeq viking, but in her portrait she's... a male Seeq viking with lipstick.
* In ''Franchise/KingdomHearts'', the Bouncywild, the DistaffCounterpart of the monkey-like Powerwild, has blonde pigtails and a bow.
* Nintendo's Miis have all the same facial and hair combinations, however males wear a shirt and pants and females wear a dress that flares out.
* Three of the four female Kongs in the ''VideoGame/DonkeyKongCountry'' series have long, blonde hair. The only one to not have it is Wrinkly Kong, as she is an old lady.
* [[MemeticBadass Pablo Sanchez]] of ''VideoGame/BackyardSports'' has a baseball cap, is bald, and wears shorts. In every game except Skateboarding where he wears a helmet.
* ''Franchise/TheLegendOfZelda'':
** ''VideoGame/TheLegendOfZeldaSpiritTracks'': Why can you tell Zelda apart from the other [[AnimatedArmor Animated Armors]]? ''Because she's freakin' [[color:pink:pink]]!!''
-->'''Random Phantom''': (to Zelda) YOU SEEM CUTER THAN USUAL. WHY.
** In ''[[VideoGame/TheLegendOfZeldaOracleGames Oracle of Ages]]'', the Maku Tree in Labrynna has long eyelashes, ganguro-style makeup, and a large flower low in her branches.
** In ''VideoGame/TheLegendOfZeldaTwilightPrincess'' the [[http://zelda.wikia.com/wiki/Female_Monkey Female monkey]] is distinguished from the others by a rose on her head.
* Mocked in ''VisualNovel/AceAttorneyInvestigations'' by Edgeworth, where he objects to its use on the Pink Badger, the Blue Badger's DistaffCounterpart.
-->'''Edgeworth''': I suppose this is the Pink Badger? But since it has the same design, doesn't it seem forced to call this one a female?\\
'''Kay''': You think so? I mean, just look at how long her eyelashes are!\\
'''Edgeworth''': That's the only difference.\\
'''Kay''': And the fact that she's pink.\\
'''Edgeworth''': Yes, and?\\
'''Kay''': And her lips are red! See, lipstick!\\
'''Edgeworth''': (thinking to himself) ''What? She has nothing to say about the giant pink ribbon, or is that too obvious?''
* ''Franchise/SpyroTheDragon'':
** In ''VideoGame/SpyroAHerosTail'', girl dragon (and {{expy}} of Amy Rose) Ember is [[PinkGirlBlueBoy pink]], has a necklace, and has a heart-shaped tail tip. Spyro and Flame on the other hand are purple and bright red.
** In ''VideoGame/SpyroYearOfTheDragon'', some of the female dragons hatch out of their eggs ''already wearing a bow''.
* All ''VideoGame/{{Creatures}}'' games exhibit a mix of this (many official breeds differ by hairstyle, two have ''makeup'' on the females, and one C1 breed had pinkish females and bluish males) and more typical sexual dimorphism (different coat colors, horns on male Fallow Norns.)
* Bandage Girl in ''VideoGame/MeatBoy'' series wears a distinctive flower.
* ''VideoGame/AnimalCrossing'':
** Your player character will wear a t-shirt and shorts if they're a boy, and a dress if they're a girl. They'll also each have a different set of hairstyles (though at one point in the game, girls can get boy haircuts and vice versa.)
** In the UsefulNotes/Nintendo64 original title, and its updated versions on the UsefulNotes/NintendoGamecube, the female protagonists wore cone shaped hats while the boys wore viking helmets, that they were unable to remove. Custom hats in future games still use these designs.
** On the NPC end, animals will have either blue or pink name boxes during conversations depending on their gender.
** Averted starting with ''New Leaf'', where it's possible to cross-dress and even make your character look exactly like the opposite gender. [=NPCs=] wont react to it, but other players might. Also getting your hair done a certain amount of times at Shampoodle (New Leaf only) will unlock opposite gender hair options.
* ''Gokujou VideoGame/{{Parodius}}'' and ''VideoGame/{{Otomedius}} G'' have the giant female Moai head Yoshiko, who wears lipstick and earrings. The latter game reveals that it's not her real face.
* In ''Chack'n Pop'', Miss Chack'n can be told apart from Mr. Chack'n by the ribbon in her hair.
* In ''VideoGame/AngryBirds'' some later bird additions and merchandise have stylized "feminine" eyelashes and bows on their heads.

* "Big Sis Prinny" from ''VideoGame/DisgaeaHourOfDarkness'' is pinkish-purple rather then blue like the generic Prinnies.
* Isaac's female alter-egos from ''VideoGame/TheBindingOfIsaac'':
** Maggy has a blonde wig with a bow in it.
** Eve has a scraggly black wig, ExcessiveEvilEyeshadow, and a dead bird in her hair that could be mistake for a bow at a glance.
** Lilith has a long, red wig.
* ''VideoGame/StarFoxZero'' features a pink colored vixen. She also has a TuftOfHeadFur, though not the FurryFemaleMane Krystal has.
* Determining a Sackboy from a Sackgirl in ''VideoGame/LittleBigPlanet'' is based solely on what clothing and design options the player places on them.
* The main characters of ''VideoGame/{{Snipperclips}}'' seem to be male and female based solely on their tertiary traits. One of them wears a bowtie and sneakers, while the other has long eyelashes, and wears a scarf and flared heel boots.
* Rebecca, the female hedgehog from ''VideoGame/ShadowOfTheWoolBall'' and its sequel, can be recognized as female because she has visible eyelashes and long hair.
* ''VideoGame/{{Cuphead}}'' cleverly uses the same accessory as one for male and female at the same time. One of the bosses fought is a large domino with a masculine top half named Pip, and a feminine bottom half named Dot. There is a bow in the middle of the domino that would serve as a [[RingAroundTheCollar bow tie]] for Pip, as well as a hair bow for Dot.

* In ''VideoGame/BoxBoy'', the only difference between Qucy and the other two boxes is that she's got a big grey head bow. That being said, Qbby (and later Qudy and Qucy herself) can avert this due to the gendered clothing options not being restricted to any one character.
* In Benji's Bananas, Benji's girlfriend differs from his by the [[FlowersOfFemininity flower in her hair]], her eyelashes, and her clothes. Benji himself is just a normal monkey.
* The ''VideoGame/{{Henry Stickmin|Series}}'' series has Ellie Rose. Before she speaks, she has a feminine-style haircut and a red mouth for lipstick to showcase that she is female.
* Lady X Subsistance from ''VideoGame/RumbleRoses'' to emphasize she is supposed to be a female despite being a robot has wide hips, high heeled shaped feet, and feminine eyes. The "Substance" model appears a bit more human-like but is still obviously a robot and has long hair alongside clothes that shows her belly and covers the vital spots females have to cover.
* ''Franchise/StreetFighter'' has human females and thus through their figures and clothing it is easy to tell their genders. The girls do however have a trait the men don't in that they are not allowed to look unpresentable thus they don't [[https://i.imgur.com/IylRUes.jpg appear goofy]] or their [[https://i.imgur.com/pSKBdGe.jpg jaws do not deform]] when hit by certain attacks.
* Oniko from ''VideoGame/PuyoPuyo'' looks much like her crush Onion Pixie, but with a red ribbon on her head, a dress, and visible eyelashes.
* In ''VideoGame/LaterAlligator'', it's easy to tell that Pat's baby sister Angelface is female because she wears a pink bow.

* Female Website/{{Neopets}} can be distinguished from male ones by the fact they have visible eyelashes.

* Ojo from ''Series/BearInTheBigBlueHouse'' is female, but lacks any eyelashes or makeup, wears no clothing or accessories, has a TomboyishVoice and her fur is a darker shade of orange than Bear's, inverting PaleFemalesDarkMales. Even her name sounds like a boy's name. It's enough to cause some ViewerGenderConfusion.

* Ojo from ''Series/BearInTheBigBlueHouse'' is female, but lacks any eyelashes or makeup, wears no clothing or accessories, has a TomboyishVoice and her fur is darker than Bear's, inverting PaleFemalesDarkMales. Even her name seems more suited to a boy. It's enough to cause some ViewerGenderConfusion.

* Throughout the ''VideoGame/XenobladeChronicles'' series, determining the gender of Nopon (who are all shaped like furry spheres) comes down to contextual clues. In ''VideoGame/XenobladeChronicles2'', Zeke reveals that this applies InUniverse as well, stating most people can only tell Nopon boys from girls by their clothing. The boot is on both feet, however, as the same scene reveals party member Tora thought Mòrag was a man. She takes it quite personally (while she does speak with a gruff tone and is never seen out of her military uniform, a human would be unlikely to make that mistake). The setting even takes pronouns into account: in Japanese, Mòrag uses collective or masculine pronouns as befits her rank; in English, the [[YouNoTakeCandle Noponic dialect]] doesn't use them, so Tora never knew the difference between "he" and "she".

* Ojo from ''Series/BearInTheBigBlueHouse'' is female, but lacks any eyelashes or makeup, wears no clothing, has a TomboyishVoice and her fur is darker than Bear's, inverting PaleFemalesDarkMales. Even her name seems more suited to a boy. It's enough to cause some ViewerGenderConfusion.

* Ojo from ''Series/BearInTheBiBearInTheBigBlueHouse is female, but lacks any eyelashes or makeup, wears no clothing, has a TomboyishVoice and her fur is darker than Bear's, inverting PaleFemalesDarkMales. Even her name seems more suited to a boy.

* Throughout the ''VideoGame/{{Xenoblade|Chronicles}}'' [[VideoGame/XenobladeChroniclesX series]], determining the gender of Nopon (who are all shaped like furry spheres) came down to contextual clues. In ''VideoGame/XenobladeChronicles2'', Zeke reveals that this applies InUniverse as well, stating most people can only tell Nopon boys from girls by their clothing. The boot is on both feet, however, as the same scene reveals party member Tora thought Mòrag was a man. She takes it quite personally (while she does speak with a gruff tone and is never seen out of her military uniform, a human would be unlikely to make that mistake). The setting even takes pronouns into account: in Japanese, Mòrag uses collective or masculine pronouns as befits her rank; in English, the [[YouNoTakeCandle Noponic dialect]] doesn't use them, so Tora never knew the difference between "he" and "she".

* Noticeable eyelashes and sometimes eyeshadow. Even if the animal isn't anthropomorphized in any other way, even if she isn't a mammal, having long eyelashes is one of the quickest ways to establish that this character is, in fact, female.

* Apart from the general traits that are rooted in reality (such as MasculineLinesFeminineCurves), here are some common traits:
** Guys tend to have smaller eyes (smaller eye holes as well as smaller irises), and their eyelashes aren't prominent. Girls tend to have larger eyes, and their eyelashes are drawn as prominent clumps.
** Guys tend to have TsurimeEyes, while girls tend to have TaremeEyes. Guys with tareme tend to be cheerful, innocent boys, while girls with tsurime tend to be mature, mysterious or seductive ladies.
** Guys tend to have high cheek bones, making them look more mature. Girls tend to have lower cheekbones, making them look younger.
** Guys tend to have blockier fingertips. Girls's fingers tend to be shrink toward the tips, making them look daintier.
** Guys tend to have wider necks proportionally to their heads; small necks in male characters tend to denote youthfulness or immaturity. Girls tend to have smaller necks propotionally to their heads, some have necks so small that it's a miracle that they could withstand such big heads.
** Guys tend to have a dark, yellowish or brownish complexion; pasty guys are almost certain to be some sorts of [[DudeLooksLikeALady femboys]] or halfs. [[PaleFemalesDarkMales Girls are paler]] and more pinkish; some designs may have girls with [[UnkemptBeauty natural rosy cheeks and lips]].

** Guys tend to have a dark, yellowish or brownish complexion; pasty guys are almost certain to be some sorts of [[DudeLooksLikeALady femboys]] or [[MixedAncestry halfs]]. [[PaleFemalesDarkMales Girls are paler]] and more pinkish; some designs may have girls with [[UnkemptBeauty natural rosy cheeks and lips]].

[[caption-width-right:300:They've got bows, makeup, pink attire, skirts, visible eyelashes, and high heels. Yup, [[DisguisedInDrag definitely girls]]. I swear that's WesternAnimation/{{Minnie|Mouse}} and WesternAnimation/{{Daisy|Duck}}.]]

* In ''Webcomic/TheInexplicableAdventuresOfBob'', after one of the [[StarfishAliens lobster-like]] [[SpacePirates Pirates of Ipecac]] is suddenly revealed as [[SamusIsAGirl female,]] she is then always portrayed with [[http://bobadventures.comicgenesis.com/d/20070828.html eyelashes and slightly rounded curves on all the pieces of her shell.]] Up till this point, both pirates had been drawn identically. Since the transition occurs ''between panels of the same strip'', Cleaveland is probably [[LampshadeHanging Hanging A Lampshade]] on this trope.

* ''Franchise/{{Pokemon}}'': Gender-specific physical differences were created for some Pokémon in ''VideoGame/PokemonDiamondAndPearl'' and carried over to future games. Most of them, as well as earlier attempts at portraying sexual dimorphism in the franchise, fall under SecondarySexualCharacteristics, being random changes or based on real life animals, but a few, such as female Wobuffet's lipstick, are closer to this, via RuleOfFunny.

* ''Franchise/{{Pokemon}}'':
** Gender-specific physical differences were created for some Pokémon in ''VideoGame/PokemonDiamondAndPearl'' and carried forward for future games (for example female Pikachu have heart-shaped tails and male Torchic have small dots on their backsides). Fortunately, they mostly tend to be arbitrary markings or based largely on gender differences in reality, so good on Game Freak's part. Female Wobbuffet appear to wear ''lipstick'', but [[RuleOfFunny it's just for the lulz]]. Mercifully, none of the [[SuperMode Mega Evolutions]] or [[UndergroundMonkey Alolan Forms]] have visual gender differences, even though their basic forms did.
** Prior to this, the few gender-dimorphic Pokémon had their obviously different male and females listed as '''separate species'''! Male Volbeat is red and has a glowing tail, female Illumise is purple and doesn't. As a maybe-intentional subversion/inversion of the typical blue/pink color scheme, Nidoran-line females are blue/indigo, but the males are ''[[RealMenWearPink pink/purple]]''. Shiny variants avert this, though (except for Shiny Nidoqueen, which is greenish).
** Note that the Pokémon that get different stats based on their gender ''are'' actually very different. The male and female Nidoran lines learn entirely different moves and have differing stats. Some Pokémon only exist as a certain gender (no male Combee will ever evolve into a Vespiquen, for example). [[VideoGame/PokemonXAndY Generation VI]] introduced Meowstic, who share the same Pokedex entry but have different colorings, completely different movesets, and (if leveled up to learn moves as normal) lend themselves to completely different styles.[[note]]In a subversion of the normal RPG rule of thumb, the female's the attacker while the male favors support techniques.[[/note]]

* Noticeable eyelashes and sometimes eyeshadow.

* Becoming a Super Mutant in ''VideoGame/{{Fallout}}'' causes one to lose secondary sexual characteristics and become sterile, muscle-bound, and masculine-looking. Two female Nightkin in ''VideoGame/FalloutNewVegas'' accessorize to look feminine: Lily wears a gardening hat and shawl, while Tabitha sports a pair of heart-shaped pink eyeglasses and a blonde wig.