Jonathan "Jojo" Joestar

Dio Brando (the Big Bad) ambushes his adoptive brother Jonathan Joestar (the main protagonist) on his honeymoon with the intention to claim his body (as Dio's body was destroyed in their previous battle) after his hatred for him has twisted so far around it has become Villain Respect, believing no one else would be worthy.<br>He mortally wounds Jonathan and latches onto him with his prehensile veins, but Jonathan uses the last of his strength to grab Dio's head and hold him close to keep him in place, and Dio can only beg his foe to let him go while promising him immortality... and then he realises that Jonathan is already dead (with a smile) and is genuinely affected by Jonathan's death. Even though he does indeed behead Jonathan's body and attach his own head to it afterwards, he sounds like he's about to cry when he realises that Jonathan is actually dead.<br>Jonathan's last words prove that Dio's newly found brotherly love, in spite of everything, was returned, embracing his Arch-Enemy and adoptive brother as he kicked the bucket.

Example of:
The Hero Dies