Beach Episode: A chunk of the episode is set along a beach in the human world.
Call-Back: When Tsukune points out that vampires can't go in the water, Nazo butts in and saves everyone time by telling the audience to watch Episode 5.
Fantastic Racism: Rubi tries killing Tsukune the moment she sees him, convinced that he is just another human out to destroy Witch Hill. The fact that he's Yukari's friend perplexes her and adds him being a threat to their kinship as witches as a new reason to kill him.
Glamour Failure: Nekonome is reminded to hide her tail twice.
Mistaken for Aliens: Inverted. With the exception of Yukari (who she sees is a witch) and Tsukune (who actually is a human), Rubi thinks the rest of the Newspaper Club are all human.