Please don't list this on a work's page as a trope. Examples can go on the work's YMMV tab.
Nightmare Fuel: Sim Ant
You play as a single ant trying to build a colony. The overworld is covered with hazards like puddles, large bugs, and most importantly, spiders. Just try running your ant too close to a spider. If the description of your death ("As the spider's venom courses through your veins, you feel like you have been set on fire,") doesn't scare the shit out of you, the accompanying head-on view of the spider, gnashing its fangs will. The game is rated E.
Just be thankful that despite looking like a Wolf Spider on the playing field, the face you see when you die is really that of a Jumping Spider. As a Google Images search will efficiently tell you, the face of a Wolf is a lot freakier than that of a Jumper.
Perhaps that's what the Clown button was put there for? Said the spider: "I'm so cool, I can hardly believe it!"
The Spider and the Lawnmower. Horrible, horrible things.
You can take out the spider and the ant-lions with a big enough horde. They respawn and you will take huge casualties, but it's very satisfying.
Actually, spider-hunting can be a very effective way of gaining food on a map where your colony's developed (and crammed with soldiers, of course) and the food supply has dwindled for one reason or another.
On the original PC version, the sound effects are MUCH freakier than the SNES version. Events (such as having a follower emergency-feed you) jump out of nowhere with unsettling visuals and sounds, leading to many an unintentional Jump Scare.
A sudden, flash-stepping spider appearing out of nowhere and eating you, accompanied with a close up of its face. Even positive things like getting fed are accompanied by Nightmare Fuel.
The spiders are really terrifying. The creepy close up of its face combined with the crunching sound don't leave a good impression.