Betty's father's head turning into a grampohone, followed by her mother taking the voice track out and replacing it with a recording of "Oh Du Lieber Augustin".
The flower trying the bratwurst and dying as a result.
Betty and Bimbo are so scared of the walrus, their colors briefly invert.
Boop-Oop-a-Doop has a Running Gag of Bimbo selling peanuts to a baby and the baby keeps telling him no. When the baby finally relents, Bimbo sees that there's a hole in his basket and that there are no peanuts. The baby starts spewing angrish until a hand puts a "CENSORED" sticker over his mouth.
The Bum Bandit has Bimbo trying to keep his gun steady while shooting a tin can. When he shoots at the can, an injured bird falls from the sky. When he shoots at the bird, a cow drops from the sky. Bimbo then fires into the sky, which finally punctures the can.
Whenever a song gets to the final chorus with the words morphing into characters or objects, expect at least one chuckle.
Put on Your Old Gray Bonnet has a scene of the flute player falling asleep while the orchestra plays the title song. The conductor whacks him with his baton and he instead starts playing "Yankee Doodle".
In the Shade of the Old Apple Tree has a scene at the beginning where all the workers leave the factory for lunch and the building shrinks down to a doghouse when everybody leaves.
A scene where two (live-action) movies are being shown side by side to save time on the program, only for the characters in the movies to tell the ones on each side to shut up and bicker. The baker on the left side then throws a pie which hits a couple on the right side.
Mariutch has a scene where the Italian immigrant finds that his wife is a "hootch-a-ma-kootch" dancer.
The scene during the final chorus where said wife appears to be doing the dance and stripping in silhouette, the man raises the curtain to reveal that it's three acrobats.