History TertiarySexualCharacteristics / WesternAnimation

** ''WesternAnimation/HouseOfMouse'' lampshades this by mentioning Minnie's obsession with hairbows, sometimes making it part of the plot. In "Mickey's Big Break", Mickey and Donald had to [[DisguisedInDrag crossdress as their own girlfriends]] to replace a picture of Minnie and Daisy they broke while playing football inside the house.

* ''WesternAnimation/WonderPets'' has a toy-around with this. The hero, Linny the Guinea Pig, wears a baseball cap and cape. She's a girl, though. (The fact that her name sounds like "Lenny" doesn't help the ViewerGenderConfusion, of course...)
* ''WesternAnimation/SpongeBobSquarePants'' has female style eyelashes. They're probably there to make him look even cuter, as he has an endearing child-like demeanor. Real-life sea sponges are hermaphroditic animals that reproduce asexually. The show's creator was a Marine Biologist, [[ShownTheirWork so...]]
-->'''[=SpongeBob=]''': Can you reproduce by budding?
** When Mr. Krabs tells [=SpongeBob=] that a hat he's wearing makes him look like a girl, [=SpongeBob=] happily takes it as a ''compliment'':
--->'''[=SpongeBob=]''': (starry-eyed) Am I a ''preeetty'' giiirl?
*** Later when Krabs tells him that he ''isn't'' beautiful, [=SpongeBob=] lets out a sad, little "I'm not?" and gets tearful.
** Lampshaded sometimes when some characters actually ''do'' mistake him for a girl, or aren't sure what gender he is.

* ''WesternAnimation/{{Recess}}'':
** Spinelli [[OneSteveLimit shares a first name]] with [[AlphaBitch The Ashleys]] clique... and nothing else. She does wear a skirt, but she gender-neutralizes it with her very masculine boots, coat, and hat. She has pigtails though, so she's not completely neutralized.
** The show actually subverts the cartoony way of not really having any tertiary sexual characteristics, like the eyelashes, as none of the girls (or if anyone's wondering, the boys) have any. The only characteristic the girls are given to look different are that they're drawn with full lips (with the exceptions of Cornchip Girl and a few others). In fact, the ''only'' female character drawn with eyelashes is Miss Grotke. She probably wears mascara.
* Oddly, the Russian animated series ''Animation/NuPogodi!'' features a little hare, who, despite sporting long eyelashes, big blue eyes, pink cheeks, engaging in girly activities such as watering flowers, and being [[CrossdressingVoices voiced by a woman]], the artist [[WordOfGod insists]] is a male. It's a ZigZaggingTrope where Hare is concerned- There's no ViewerGenderConfusion in this case in homeland Russia, because the hare in question is called/named just the Hare, and the Russian word for "hare" ("Zayats") is masculine by default, implying that the Hare is indeed a boy. Don't ask. He is also wearing shorts. Unfortunately, the rest of the world who watched the exported version of the show were rightfully confused given that the English VA preserved Hare's high-pitched voice, left the scenes where he engages in feminine activities uncut, and girls, and just assumed the pants to indicate a tomboy. The trope is played straight with a lot of other anthropomorphic animals appearing in the series, whose gender is mostly determined through the pants vs. skirt method.
* On one of ''WesternAnimation/TheSimpsons'' Halloween episodes, the aliens Kang and Kodos are revealed to be siblings -- and male and female respectively. Both have identical appearances and deep voices (though if you listen carefully, Kodos's voice is ''slightly'' higher).
* Played with for the [[RuleOfFunny inherent humor]], in ''WesternAnimation/TheVentureBrothers''. Doctor Girlfriend's physical tells are obvious -- she likes the same pink dresses and pillbox hats Jackie Onassis wore. But if you only heard her and her very masculine [[CuteButCacophonic smoker's voice...]]

* ''WesternAnimation/ChallengeOfTheGoBots'' averted this by assigning gender to the (presumably genderless) toys in a completely arbitrary fashion. Small Foot in particular has no human-esque gender indicators apart from her voice.
* Zigzagged in ''WesternAnimation/TheBackyardigans'': Tasha in 'reality' is a typical girly-girl in a cute dress and takes similar parts in the pretend adventures, while Uniqua wears overalls more usually characteristic of males or {{Bokukko}}s and gets an impressively gender-neutral selection of roles. Then again, she is also [[PinkGirlBlueBoy pink with darker spots]].
* Jerry and Nibbles/Tuffy of ''WesternAnimation/TomAndJerry'' inverts this with his long eyelashes and cute face, leading to ViewerGenderConfusion for some. Female cats in the shorts had long eyelashes, wore lipstick, bows and sometimes female clothes.
* ''WesternAnimation/TheAmazingWorldOfGumball'':
** Inverted majorly: eyelashes aren't exclusive for female characters. Darwin the fish has feminine eyelashes and a rather girlish voice, although his VA is a boy. Contrast with other female characters like Tina (a relatively realistic T-rex), Masami (a cloud)[[note]]This was lampshaded once by Gumball not being sure if she's really a girl[[/note]] Molly (a brontosaur), and Carmen (a cactus), who have very gender-neutral appearances. Several other male characters also have eyelashes (Richard the rabbit, Alan the balloon, the donut cop) while only about half the female ones do.
** As for the Wattersons, despite the fact that eyelashes aren't a telltale sign, Nicole and Anais's eyes are completely round, as opposed to the guys' eyes which looked like straight tubes. [[ArtEvolution All of their eyes are rounded in later seasons]] to make them all cuter, and now all eyes are perfect circles instead of ovals
** That said, any time a flashback shows Nicole as a child, she's always wearing a red/pink bow, likely to obscure how she [[StrongFamilyResemblance looks almost identical]] to her own ''son.''
** In the episode "The Blame," Gumball impersonates his mother by wearing her outfits and drawing eyelashes on his face. He doesn't account for when he closes his eyes and the lashes don't match, though.
* ''WesternAnimation/{{Arthur}}'' has at least one. Similar to the ''Wonder Pets'' listed above, there was a young female character with a gender neutral voice and a baseball hat nickname "W.D."; she also has a gender neutral haircut and wears masculine clothing, to emphasize she's a tomboy. Her name is "Wilhelmina", but no one calls her that.
* Mostly averted in ''WesternAnimation/ThePenguinsOfMadagascar'' and played with in "Miss Understanding". Due to some mistake, Skipper's led to believe that he is actually female. Once he accepts it, he promptly puts on a big pink bow.

* From around 1947 to 1955, WesternAnimation/MightyMouse was given eyelashes. They were absent from the 1959-61 TV-budget shorts and the Filmation series then reinstated in the Bakshi series.

* ''WesternAnimation/BunnyMaloney'' stars titular Bunny, a pink male rabbit, and his girlfriend Candy, a pink female rabbit. Bunny looks like the "men" silhouette on a restroom door if it had rabbit ears and a tail; Candy has a much curvier figure, {{Blush Sticker}}s, eyelashes, and a red scrunchie on her left ear.
* While the female Franchise/CareBears tend to have lighter, more pastel colors than their male counterparts, color is not always a giveaway, and, of course, more than one bear has flipped gender between generations. According to TCFC, the way to distinguish males from females are the eyebrows, but it's not that females have them and males don't -- it's that females have three, while males have only two.

* In ''WesternAnimation/StevenUniverse'' most female characters and gems aren't drawn with eyelashes (and most gems apparently have no breasts). Ironically, the one who does have noticeable eyelashes is the [[TheBrute very masculine, evil]] [[TomboyishName Jasper]]. Steven himself averts the cliche that only girls wear pink, as his clothes and powers are both pink.
* Most boys in ''WesternAnimation/AsToldByGinger'' have no eyelashes. Minor character Ian has eyelashes, possibly to emphasise that he is a "PrettyBoy", while the campy and effeminate Brandon also has eyelashes.
* Darla "The Geek" Gugenheek from ''WesternAnimation/TheAdventuresOfSamAndMaxFreelancePolice'' is drawn without lashes, further emphasizing her tomboyish appearance.
* In ''{{WesternAnimation/Wildfire}}'', all the foals are given long eyelashes, including the colt Brutus.
* Subverted with Little Sneezer from ''WesternAnimation/TinyToonAdventures''. Eyelashes? check. High-pitched voice? Check. But make no mistake, he's a male.
* Subverted in the ''WesternAnimation/LooneyTunes'' short "WesternAnimation/BackAlleyOproar". Sylvester (before his {{Flanderization}} to Tweety's ButtMonkey) hands off a bit of sheet music to a big orange cat who looks stereotypically male (and the thing is, it's hard to say exactly ''how''). '''She''' proceeds to sing a lovely soprano aria from an opera -- before getting clobbered with a shotgun butt and staggering off the roof.
* An old animation trope was to draw all babies with eyelashes to make them look youthful and innocent. This can be seen in many shorts from the early-to-mid 1900s but has gone out of style since. This is why Tweety from ''WesternAnimation/LooneyTunes'' has prominent eyelashes--he's a chick.

* ''WesternAnimation/TheBeatles'': In "Not A Second Time," a very off-model Paul is given eyelashes as the boys rehearse their song.
** In a singalong host segment when John asks Ringo for props to support a romantic ballad, Ringo emerges in a ballet dancer's tights ([[IncrediblyLamePun "I'm a ballad dancer!"]]), with eyelashes to boot.
* The 1939 [[WesternAnimation/LooneyTunes Warner Bros.]] cartoon "Screwball Football" has a team kicking off to start the game. The entire line is posed post-kickoff and then performing a cutesy dance recital and finishing it with a curtsy.
* ''WesternAnimation/BeCoolScoobyDoo'' had Fred identifying the Mystery Machine as "she." Shaggy questions "The Mystery Machine is a girl??" After which Scooby looks under the chassis and asks "How can you tell?"
* WesternAnimation/CourageTheCowardlyDog is a pink, male dog.

* While many of the characters in ''WesternAnimation/PacMan'' play it straight, Pac-Baby does not. Despite the tuft of hair, the pastel colors, and the bow, an episode showing an older Pac-Baby reveals him to be [[GenderFlip male]].

* Various non-human characters from ''WesternAnimation/OggyAndTheCockroaches'' have pronounced eyelashes and other human-like feminine features:
** Cats: Olivia (Oggy's love interest), Monica (Oggy's twin sister), Oggy's mother and Oggy's unnamed female relative all have eyelashes, with Olivia wearing a bow, while Monica has a GirlishPigtails and pink rollerskates; Oggy's grandmother is an exception but she does wear a dress and has pink nail-polish.
** Others: Lady K (the roach in Olivia's house and Joey's love interest) and the fat bee that Joey had a crush on have eyelashes, thick lips and [[NonMammalMammaries breasts]]; Dee Dee's cow and one of Oggy's pet snail have eyelashes and thick lips.

* ''WesternAnimation/TheSmurfs1981'':
** The evil wizard Gargamel takes his revenge on the Smurfs by creating a 'Smurfette' to trick them. While the male Smurfs all wear white pants and had very little hair, the original Smurfette had long black hair and a white dress. In a nutshell, the Smurfs disapproved of her because she wasn't a real Smurf. So Smurfette visited Papa Smurf... and he turned her into a pretty blond in a sexier white dress, heels, long eyelashes, and a flirty attitude. No wonder poor Smurfette has [[TheSmurfettePrinciple a trope named after her]] -- and it isn't a positive one either.
** A second and third Smurfette (named "Sassette" and "Nanny Smurf" respectively, to avoid confusion) were introduced latter. Sassette (who is also an artificial Smurf) wears overalls, like Handy, but hers are pink and she has long, red hair, which she wears in GirlishPigtails. Likewise, Nanny Smurf (Who knows where she comes from) is identified as female by having hair and a female voice. It should also be noted that all three Smurfettes have notably smaller noses than the male smurfs.

* The miniscule difference between VideoGame/PacMan and Ms. Pac-Man was parodied in ''WesternAnimation/DrawnTogether'', where Pac-Man reveals that he ''is'' Ms. Pac-Man when he puts on the bow.
* Haley Long in ''WesternAnimation/AmericanDragonJakeLong''. She doesn't have the bow, but when she transforms, she always has her hair. Though this seems to be true for all the characters when they transform, male or female, her dragon form is also ''pink'' (in season 1 she was purple, which is considered more unisex though leaning towards feminine than Jake's red tone). When Fu Dog is showed macking on a female dog, she usually has a bow, lipstick, and/or a sparkly collar. Hey, Fu digs the girly-girls.
* Franchise/CareBears come in all colors - though a female Bear's fur tends to be more noticeably pastel. And nearly all of the pink-furred characters are female by default. In some series, they even wear girlie clothes too. (Note that this applies to cases where [[ViewerGenderConfusion the writers had made up their minds]] as to who was what.) Subverted with Swift Heart. Even though she had a very obviously feminine voice and behavior, ''nobody'' realized she was a girl since she's a blue rabbit with no bow or anything. Swift Heart might actually justify this trope on a meta-level.

* On the [[WesternAnimation/ClassicDisneyShorts Disney side]], in early cartoons, WesternAnimation/MinnieMouse and WesternAnimation/DaisyDuck would be virtually indistinguishable from their respective beaus if it weren't for the lashes, hair bows, heels and skirts. In an interesting footnote, Minnie seemed to favor going topless, while Daisy's skirt barely covers her bare bottom. Not that there's anything there to hide, but...
** Interestingly enough, take a look at some of the [[FollowTheLeader characters who popped up in Mickey and Minnie's wake]] and you'll come away with the impression that Minnie was the TropeMaker for "Well, she's got a bow on her head!"
** More recent designs of the two characters have toned down their more obvious tells. In particular, Daisy now looks more graceful and feminine (for a waterfowl) and less like WesternAnimation/DonaldDuck in a dress. She has also acquired some NonMammalMammaries. [[OlderThanTheyThink Even in the classic days]] various artists differed in their ability to make them look feminine. The ArtEvolution went towards the obvious side of this trope.
** ''WesternAnimation/HouseOfMouse'' lampshades this by mentioning Minnie's obsession with hairbows, sometimes making it part of the plot. In "Mickey's Big Break", Mickey and Donald had to [[DisguisedInDrag crossdress as their own girlfriends]] to replace a picture of Minnie and Daisy they broke while playing football inside the house.

* ''WesternAnimation/YinYangYo'' is all over the place. Yin herself has the eyelashes and the hairbow. Her friend Lena seems to get by on just lipstick. The Aardvark Princess has the clothing, but neither lipstick nor bows. Villain Smoke has the secondary sexual characteristics. No bows, no lipstick, but a [[Franchise/SailorMoon Sailor Scout]] type outfit, and big breasts. Villain Saranoia has the secondary sexual characteristics, as well as the lipstick. Carl's mother has the lipstick but not the clothes or the breasts. The Chung Pow Kitties all have the eyelashes and bows. Which is justified, because they communicate only with kitten-like meows. One really couldn't tell without the eyelashes and bows.
* ''WesternAnimation/{{Chowder}}'':
** Panini has the eyelashes and the girly color scheme, but wears a sweatband/scrunchie around her ears rather than a bow. Chowder in comparison is light purple, which is often considered feminine though can be unisex. Chowder and Panini are the same species but look nothing alike.
** Also, in the "Thrice Cream Man" episode: compared to the titular creature, which is an aqua-blue blob of [[LetsMeetTheMeat living thrice cream]], the Thrice Cream Woman is pink (strawberry-flavored?) with lipstick, eyelashes and [[NonMammalMammaries breasts]].

* In ''WesternAnimation/TheLandBeforeTime'', females tend not to appear particularly different from males, although in some art, Ducky and Cera are given near-pastel color schemes. In the fourth film, a guest character called Ali is written in, who is the same age and species as Littlefoot, although a different gender. To get the effect, Littlefoot's design is copied, but the eyelashses are lengthened slightly, eye colour is changed from red to blue and her overall colour is slightly redder. Another new female character, an Oviraptor, is added into [[RecycledTheSeries the series]] -- and she's ''pink''. And she's named "Ruby", presumably to help the colorblind. Amusingly, Ali's appearance evidently isn't enough to establish her gender to the other characters -- Ducky ''checks''.
* Most of the female robots in ''WesternAnimation/{{Futurama}}'' are like this (with some notable exceptions).
** Bender gets hammered into Fembot shape in one episode, including reworking implied genitals that he has never demonstrated before or since-- his ''lack'' of such was vital to saving him from the Space Amazons. Another episode had a robot with an obvious "fembot" figure that turned out to be anything but... at least until "she" finished her payments. And the Crushinator may be a piece of big, clunky Lunar farming equipment, but she's still pink, sporting pigtails, and still female... still has a woman's ''needs''.
** There's also the female Nibblonians, with ribbons on their eyestalks and long eyelashes -- though oddly enough, their race also has a more realistic sexual dimorphism in the males having larger canines.
** The bows and eyelashes are also somewhat {{justified|Trope}} here, as that makes the girl Nibblonians ''even [[RunningGag cuter]]!''
* ''WesternAnimation/LiloAndStitchTheSeries'' has Angel, who is a copy of Stitch but with a very high-pitched voice, pink fur, long eyelashes, an hourglass figure, and long antennae that look like hair. She has a heart marking on her back in her first episode. Her special power involves singing, which causes [[HeelFaceTurn anyone that used to be evil]] to [[FaceHeelTurn turn evil again]].
* Female Irkens in ''WesternAnimation/InvaderZim'' have long eyelashes and curled antennae. Tak and Invader Tenn are the only female Irken characters (aside from Tallest Miyuki, whose episode was never finished), but several Irken with the same traits show up in crowd scenes, and are presumably female as well. To be fair, this may be the ''only'' difference between male and female Irken, as it's heavily implied they don't even reproduce sexually.
* The ''WesternAnimation/DarkwingDuck'' episode "Trading Faces" [[FreakyFridayFlip swaps Darkwing and Goslyn's bodies]]. The only physical change made to them was that Darkwing's body had eyelashes and Goslyn's body had none.
* Sartana of the Dead from ''WesternAnimation/ElTigre'' is an undead skeleton-headed woman. She wears a dress, has lipstick and eyelashes, as well as HartmanHips.
* In ''WebAnimation/HappyTreeFriends'', Giggles, Petunia and Lammy all have long eyelashes. Petunia wears a flower on her head, while Giggles and Lammy have hair bows.
* In ''WesternAnimation/{{Animaniacs}}'', Dot has a yellow flower around her ears (which sometimes appears with a pink bow), a pink skirt and on fancy occasions wears a pink dress and matching jewellery. She also has tufts of fur from the side of her face that make her look like she has a pixie-like haircut. Rita the cat has eyelashes, as does Slappy Squirrel, who also has a hat with a flower on it. On the male side, Yakko and Wakko have shorter hair than Dot, with Yakko wearing pants (oversized slacks with a large belt) and Wakko not, although he does wear a [[PinkGirlBlueBoy blue]] sweater and red baseball cap. The Hip Hippos also had a PinkGirlBlueBoy color scheme.

* In kids' show ''Dive Olly Dive'', the female submarine has curly eyelashes, pink eyeshadow, and a ''heart-shaped headlight''.
* Christmas special, ''WesternAnimation/RudolphTheRedNosedReindeer'' (the stop animation one) has Claurice, a doe that Rudolph is attracted to. How do we know Claurice is female? Well, she has no antlers for one thing, but she also has lighter fur, eyelashes and a bow on her head. Yup, definitely a female deer. In real life though, both reindeer sexes have antlers. In fact, the females shed theirs later in the year than the males do; the reindeer that have no antlers by Christmas are the old males.
* The female locomotives from ''WesternAnimation/ThomasAndFriends'' were all specifically designed after real locomotives that appear "feminine" to the show's writers. For example, Daisy has eyelashes and lipstick, Mavis and Flora both have cowcatchers and runningboards that resemble skirts, both Emily and Molly have large drive wheels, Rosie and Lady are both colored pink, and Belle's smokebox is extended in a way so that she appears to have long hair.
* In ''WesternAnimation/LooneyTunes'', Petunia Pig wears red ribbons on her pigtails.
* In ''WesternAnimation/MegaBabies'', Meg's eyelashes and ponytails distinguish her from her brothers Buck and Derrick.
* ''Franchise/MyLittlePony'':
** ''WesternAnimation/MyLittlePonyAndFriends'': In "[[Recap/MyLittlePonyAndFriendsE57TheGoldenHorseshoes2 The Golden Horseshoes, Part 2]]", the goblin mother, the only female in their family, wears a pink dress and bow, an apron, and low high heels. Her husband wears a sailor outfit.
** ''WesternAnimation/MyLittlePonyFriendshipIsMagic'':
*** As far as secondary characteristics go, males lack eyelashes and have more angular muzzles and narrower ears than females. Some are noticeably taller and stockier, but not all; for example, the exposed muzzle is the only way to determine Wonderbolt (and Shadowbolt) genders (unless they really want to let you know). There are, however, a couple of mixed and non-standard character designs in the background cast, which have been cause for endless confusion. Also, most male ponies [[note]] usually generic background ones, which in turn was one of the major inspirations for the fandom creation of Doctor Whooves [[/note]] tend to have ruffled or spiky hair while almost all female ponies' hair are completely smooth and straight. In one episode, Rarity was shown to wear false eyelashes, and she's been seen applying mascara to Fluttershy. The main difference seems to be the size of the male ponies who are always one-half or one head taller than all the females (with the obvious exception of [[LargeAndInCharge the Princesses]], of course). For ponies, who are around three heads tall, this is actually quite a large difference.
*** One particularly unconventional difference is that female ponies' eyes are usually rendered with two catchlights (and fillies have three), while the males only have two.
*** Female phoenixes have pink feathers and different head feathers. [[SubvertedTrope Or so it might seem at first]], but [[ShownTheirWork if you know much about real birds' sexual characteristics]] you will know that it is [[RealMenWearPink the smaller pink phoenix]] [[InvertedTrope who is the male]] (or just look at the confirmed-female phoenix, Philomena).
*** Male Breezies have shorter manes and rounder eyes, but still look just as girly as the females with their eyelashes.
* ''WesternAnimation/TheSimpsons'': Only women and girls have visible eyelashes.
* ''WesternAnimation/JimmyTwoShoes'': One episode has Jimmy making a DagwoodSandwich and then using it as a CompanionCube. He made it female be putting a ribbon on it.
* ''WesternAnimation/RockosModernLife'': [[https://www.youtube.com/watch?v=gssbQvhHihE "From Here to Maternity"]] involves shopping for essentials for a baby that Filburt and Dr. Hutchison are expecting to be hatched from an egg while Heffer nurtures it. Filburt and Heffer obviously expect the little tyke to be of opposite genders, considering that instances involve Heffer picking out a frilly pink dress while Filburt picks out a football jersey. There is even a montage involving Filburt playing baseball with the egg and teaching it how to shave, while Heffer dresses the egg up in a tutu and dances ballet with it and plays tea party with the ''bowed'' egg.
* On an episode of Bugged, a female bug appeared who was differentiated from the (presumably) male central bug by having bows on her antenna and lipstick.
* If it isn't otherwise obvious, the easiest way to tell if a dog or puppy on ''WesternAnimation/PoundPuppies2010'' is male or female is to look for eyelashes. If there are some, it's a female; if not, the pooch is a male. (Unless, of course, the dog's person is making up their male dogs as female for some reason.)
* ''WesternAnimation/AdventureTime'' uses this for some characters in the GenderBentAlternateUniverse--for example, the female version of [[TheDitz Cinnamon Bun]] wears a dress and bow, while the male version of [[CoolOldLady Tree Trunks]] has a mustache and a bow tie. In the normal universe, neither wears clothes. A lock of Fionna's hair is also always visible under her hat, unlike Finn who keeps it tucked in.
* In kids' cartoon series ''WesternAnimation/CaptainZedAndTheZeeZone'', the rather macho dream policeman Captain Zed has an assistant called PJ. In early series it is very, very, hard to precisely work out his/her gender, which subverts this trope. The best guess you can make is "maybe a tomboyish female. Or a teenage boy." Later series of Captain Z make it rather clearer: PJ is a normally endowed human female, physically recognizable as such by the usual secondary sexual characteristics, ie wider hips, facial features and a hint of bust.
* Often unnoticed: In ''WesternAnimation/KimPossible'' the women all have a pronounced upper lip (not supposed to indicate lipstick, as it only applies to the upper lip, and the occasional woman with lipstick has both lips accentuated) while male mouths are completely surrounded by thin lines.

* ''WesternAnimation/ToucanTecs'': The otherwise androgynous Red Leader wears a pale pink scarf. Averted with Fifi, however, as apart from her voice and name, there's absolutely no indication that she's female.
* Penelope Pitstop's ''WesternAnimation/WackyRaces'' car, the Compact Pussycat is not only various shades of pastel pink and bright red with a pair of ruby red lips as a grill, but her dashboard features lipstick application, hair dryer, and other feminine options.
* Rota Ree on ''WesternAnimation/WheelieAndTheChopperBunch'' is a yellow convertible who is also Wheelie's girlfriend. Her windshield is the form of a pink bow.
* Zee from ''WesternAnimation/MooseAndZee'' is a small blue bird who wears a [[FlowersOfFemininity flower on her head]].
* ''WesternAnimation/CliffordsPuppyDays'': Zo and Daffodil, a female cat and rabbit respectively, wear bows on their heads. Daffodil was initially [[AmazingTechnicolorWildlife pink]] before becoming white-furred later on.
* ''WesternAnimation/KipoAndTheAgeOfWonderbeasts'': The mob frogs. While male frogs all seem to have small mustaches, female frogs seem to all have fuller lips. Outside of that, they're all very similar, especially considering they all wear the same outfits.
* Spoofed in the ''WesternAnimation/SouthPark'' episode "The Cissy," when Eric pretends to be a "[[{{Malaproper}} transginger]]" girl named Erica, and the only difference is a pink bow slapped on top of Eric's hat.
* All women in ''WesternAnimation/FamilyGuy'' have noticeable lips while the men do not.
* QT-KT from ''WesternAnimation/StarWarsTheCloneWars'' is an astromech droid, the same model as the more famous R2-D2. She has the same cylindrical, domed chassis and general design of markings, except she's painted pink instead of R2's blue, her beeps and chirps are distinctly higher-pitched and "breathier" than R2's, and her name is pronounced like "Cutie Katie".
* ''WesternAnimation/AThousandAndOneAmericas'': During the SceneryPorn (accompanied with {{Introdump}}) that takes the eighteenth episode into the next setting (the Amazon River and the nearby biomes), we can see a monkey couple atop a tree's think branch. The female one has a pink flower at the top of the head, serving as a ribbon of sorts. The two monkeys are phenotypically indistinguishable otherwise.

* ''WesternAnimation/PacMan'':
** All of the male members of the Ghost Gang (including [[GenderFlip Pinky]]) wear hats. Sue, the DistaffCounterpart of Clyde, became a purple ghost with eyelashes and earrings.
** Pac-Man wears a red hat. Ms. Pac-Man wears heeled boots in addition to her usual bow, though she gains hair unlike her husband.

* ''WesternAnimation/AThousandAndOneAmericas'': Many of the wild animals Chris and Lon face in the series are very enduring and relentless, and refuse to give up. Standouts include the dire wolf in the second episode, the shark in the ninth, and the polar bear in the sixteenth.

* Summer Smith from ''WesternAnimation/RickAndMorty'' is one of the few female characters on the show to lack eyelashes and visible lips. She's even been mistaken for a man in-universe a few times.

* Subverted in ''WesternAnimation/DannyPhantom'', [[SternTeacher Mr. Lancer]] is revealed to have a sister whose photo he keeps on his desk. She has chest hair and a beard, but also long red hair and make-up. After Danny passes the test he was there to take and leaves, Lancer reveals aloud that he keeps the photo specifically as an opener for heart-to-hearts with his students. Then he wonders when anyone will ever realize the picture's just of [[DisguisedInDrag him in a dress]].

* Summer Smith from ''WesternAnimation/RickAndMorty'' is one of the few female characters on the show to lack eyelashes.

** The evil wizard Gargamel takes his revenge on the Smurfs by creating a 'Smurfette' to trick them. While the male Smurfs all wear white pants and had very little hair, the original Smurfette had long black hair and a white dress. In a nutshell, the Smurfs disapproved of her because she wasn't a real Smurf. So Smurfette visited Papa Smurf, and he turned her into a pretty blond in a sexier white dress, heels, long eyelashes, and a flirty attitude. UnfortunateImplications and gender stereotyping in one fell swoop. No wonder poor Smurfette has [[TheSmurfettePrinciple a trope named after her]] -- and it isn't a positive one either.

* ''WesternAnimation/AdventureTime'' uses this for some characters in the GenderBentAlternateUniverse--for example, the female version of [[TheDitz Cinnamon Bun]] wears a dress and bow, while the male version of [[CoolOldLady Tree Trunks]] has a mustache and a bow tie. In the normal universe, neither wears clothes.

* Parodied in ''WesternAnimation/SouthPark''. Cartman pretends to be trans in order to get his own private bathroom at school. He continues to wear the exact same clothes as usual and simply adds a bow to his hat.

* ''WesternAnimation/TheSmurfs'':

* Subverted in the ''WesternAnimation/LooneyTunes'' short "WesternAnimation/BackAlleyOproar". Sylvester (before his {{Flanderization}} to Tweety's ButtMonkey) hands off a bit of sheet music to a big orange cat who looks stereotypically male (and the thing is, it's hard to say exactly ''how''). '''She''' proceeds to sing a lovely soprano aria from an opera -- before [[SlapstickKnowsNoGender getting clobbered with a shotgun butt and staggering off the roof.]]

* In ''WebAnimation/HappyTreeFriends'', Flaky lacks the long eyelashes of the other girls. Between this and the fact that the series [[TheUnintelligible has very little intelligible dialogue]], there has been quite a bit of ViewerGenderConfusion.

** Inverted majorly: eyelashes aren't exclusive for female characters. Darwin the fish has feminine eyelashes and a rather girlish voice, although his VA is a boy. Contrast with other female characters like Tina (a relatively realistic T-rex), Masami (a cloud) [[note: This was lampshaded once by Gumball not being sure if she's really a girl]] Molly (a brontosaur), and Carmen (a cactus), who have very gender-neutral appearances. Several other male characters also have eyelashes (Richard the rabbit, Alan the balloon, the donut cop) while only about half the female ones do.