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2[[VileVillainSaccharineShow Vile Villains in Saccharine Shows]] in [[AnimatedFilms Films — Animated]].
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5* ''Animation/AdventuresInZambezia'' gives us a ruthless [[ReptilesAreAbhorrent Monitor Lizard]] named Budzo who loves the taste of [[ChildEater bird's eggs]] and is perfectly willing to betray anyone who helps him. In the past, he tore off Sekhuru's wing and he also killed the main character's mother, one of the founders of the Hurricanes. There isn't a single funny moment whenever he's on screen.
6* ''{{WesternAnimation/Balto}}'' has the despicable and murderous literal GloryHound Steele and [[BearsareBadNews the rather terrifying-looking bear]] who tries to kill the heroes at one point, with absolutely no comedic moments from these two whatsoever.
7* Franchise/{{Barbie}} [[WesternAnimation/{{Barbie}} movies]]:
8** ''WesternAnimation/BarbieInTheNutcracker'' has the Mouse King, who's voiced by Tim Curry and almost never played for laughs, attempting to chop the Nutcracker to pieces with an axe and then burn him alive in a fire.
9** ''WesternAnimation/BarbieAsRapunzel'' has Gothel, who kidnapped Rapunzel to start a war between two kingdoms that almost killed a little girl, is emotionally abusive to Rapunzel and her friends, is powerful enough to enslave dragons, and her magic makes her almost unstoppable and spends the majority of the final battle chasing everyone.
10** ''WesternAnimation/BarbieOfSwanLake'' has Rothbart, who's out to kill Odette for a majority of the movie. After rendering the Magic Crystal powerless, Rothbart blasts Daniel and Odette with his magic, killing them both until their love revives them.
11** ''WesternAnimation/BarbieAndTheMagicOfPegasus'': Wenlock is a G-rated sexual predator. He goes from kingdom to kingdom, forcing women to accept his hand in marriage or face awful consequences. He introduces himself by turning everyone in the kingdom to stone and giving Princess Annika three days to accept his proposal before the spell becomes permanent. Before the film began, he transformed Princess Brietta into the titular pegasus when her parents refused his proposal, and [[spoiler:turned three women unlucky enough to actually marry him into mute, goblin-like slaves after getting bored with them]]. He nearly kills Brietta in front of her own sister, and buries Annika alive in an avalanche when she stands up for her family.
12** ''WesternAnimation/BarbieMariposa'' has Henna, who poisons the Queen, then talks to the fairy-eating Skeezites and agrees to let them eat as many fairies as they want on the condition that she be allowed to stay ruler. She also manipulates Mariposa and almost everyone else, successfully avoiding suspicion until it's almost too late.
13** ''WesternAnimation/BarbieInTheTwelveDancingPrincesses'': [[EvilChancellor Duchess]] [[TheUsurper Rowena]] is a master manipulator and comes the closest to winning out of nearly every ''Barbie'' villain. As she slowly poisons King Randolph to death, she plays the part of a loving relative to gain his trust and cut him off from his twelve daughters, until [[spoiler:he willingly makes her his successor on his deathbed]]. In turn, she manipulates the 12 princesses, undermining their self-esteem and capabilities to help their father, to the point that they decide to leave the kingdom for their mother's secret pavilion, believing Rowena's lie that they are the cause of their father's failing health. Before they can realize their mistake, she imprisons them in their place of refuge and steals some of its magic. Upon their escape, [[spoiler:she uses the stolen magic to place a FateWorseThanDeath curse on Princess Genevieve, compelling her to dance herself to death, which Genevieve only escapes by [[PaperFanOfDoom weaponizing the paper fan]] Rowena yelled at the girls about earlier. Rowena and her henchman Desmond may well still be trapped in the dance spell to this day.]]
14** ''WesternAnimation/BarbieAsTheIslandPrincess'' has Queen Ariana. She plans to murder Antonio and his family, poisons the animals so they'll starve to death in an endless sleep, only had a daughter to serve her own ends, bribes a guard to kill Ro and her friends by knocking them into the ocean, and gained her title through marrying and killing an elderly king with a heart condition.
15** ''WesternAnimation/BarbiePrincessCharmSchool'' has Dame Devin, who is one of the few antagonists to actually murder someone. [[spoiler:In her case it was her sister-in-law.]]
16** ''WesternAnimation/BarbieInThePinkShoes'': the Snow Queen rules over the realms of ballet, and even other villains bow before her. She enforces the storytelling of every ballet, seeing to it that every story is told exactly right. Since our heroine Kristyn has found herself in the title roles of ''Theatre/{{Giselle}}'' and ''Theatre/SwanLake'', that would be [[DrivenToSuicide catastrophic]]. She freezes the life out of anyone who makes a single step out of place and brings them to her icy palace, where she puppeteers them as her perfect ballet troupe. [[spoiler:Her victims include Kristyn's non-dancer friend Hailey, who collapses from the strain once she's free.]]
17* ''WesternAnimation/{{Barnyard}}'' is, at least on the surface, an animated comedy about [[TalkingAnimal talking farm animals]] and the silly hijinks they get up to at the titular barnyard. Meanwhile, the main antagonist, [[BigBad Dag]], is a vicious, disturbingly sadistic coyote who's basically a ''serial killer'', complete with a pack of coyotes as henchmen and a necklace that has [[CreepySouvenir severed chicken's feet]] from his former victims hanging on it. He openly taunts and threatens to eat [[WouldHurtAChild a baby chick]] for [[DisproportionateRetribution calling him a "meaner"]] and threatens to kill everyone on the farm if the main character, Otis the Cow, breaks their deal (which was made after he murdered his father, no less). Appropriately enough, the comedic tone of the movie falls like a rock whenever he and/or his pack appears onscreen.
18* ''WesternAnimation/TheBoxtrolls'' is a very whimsical and funny movie. Archibald Snatcher, however, is a genuinely threatening villain, but that doesn't mean he doesn't have his own comical moments. However, when you find that he attempted to kill Eggs, [[WouldHurtAChild a child no less]], you're gonna see that he is more than what he appears.
19* ''WesternAnimation/TheBraveLittleToaster'' is a cute musical film about talking electrical appliances, but then we meet the Junkyard Magnet, who puts alive cars (and even tries to do the same with a human and the main character) in a ConveyorBeltOfDoom that takes them to the trash compactor.
20* Dark Heart from the second ''WesternAnimation/{{Care Bears|Movie II A New Generation}}'' movie is far creepier than he has any business being. A Lucifer-like corrupter who turns children into sadists that [[ThereAreNoAdults wreck a summer camp]]. He apparently is motivated only by evil, and captures the souls of the Care Bears in a giant ruby chandelier.
21** Dark Heart had nothing on the Spirit of the Book from the first movie, which wanted the whole world to stop caring and manipulated [[TheWoobie Nicholas]] into doing her work for her. She always appears as just a face in a book, turns green and always uses creepy OminousLatinChanting, comes within two people in the entire planet from succeeding, and is never PlayedForLaughs.
22* ''WesternAnimation/{{Coraline}}'' is an animated fantasy film about the titular heroine who wishes her busy parents would pay more attention to her and is, for the most part, pretty lighthearted. The main BigBad is the Other Mother, AKA [[EldritchAbomination the Beldam]], a bloodthirsty, villainous creature who lures [[WouldHurtAChild children]] to the Other World with false gifts and love to feed on their LifeEnergy. She assumes a more monstrous form as her plan unravels and it's heavily implied that Coraline is the latest in a long line of victims (Three past victims appear) and possibly the first to escape her clutches.
23* ''WesternAnimation/DespicableMe1'' has Miss Hattie and [[spoiler:Mr. Perkins]]. Unlike BigBadWannabe Vector, who ended up getting some punishment of some sort, these two are far more [[{{Pun}} despicable]], with absolutely little to no comedia moments, and even [[KarmaHoudini manage to get away.]]
24** ''WesternAnimation/DespicableMe2'' has El Macho, a presumably dead supervillain who plots to TakeOvertheWorld by brainwashing Gru's minions using a PsychoSerum that turns them into vicious purple monsters.
25** ''{{WesternAnimation/Minions}}'' has Scarlet Overkill, who personally hates the minions and tries to kill them thinking that they will be the reason why she will be defeated in the end.
26** ''WesternAnimation/DespicableMe3'' had FormerChildStar Balthazar Bratt, who plots to destroy Hollywood as revenge for firing him after he got too old to continue acting on TV.
27** ''WesternAnimation/MinionsTheRiseofGru'' has the Vicious 6, who personally all hate Gru, his minions, [[spoiler: their former leader Wild Knuckles]], and try to betray and kill them several times throughout the movie.
28* Franchise/DisneyAnimatedCanon:
29** The EvilQueen (Grimhilde) from ''WesternAnimation/SnowWhiteAndTheSevenDwarfs''. While most of the film is very cutesy and whimsical, she takes her pursuit to preserve her narcissistic self image to dark extremes. She ordered her Huntsman to assassinate Snow White and bring her heart as proof of the deed. When he failed, she took matters into her own hands, becoming a [[SeniorCreep frightening looking hag]] to trick Snow White into eating a poisonous apple that would've put her into a deep sleep. Her goal was getting her caretakers, the dwarfs to [[BuriedAlive bury her without realizing she was still alive]].
30** Maleficent from ''WesternAnimation/SleepingBeauty''. The story was very lighthearted till she [[MoodWhiplash showed up and sentenced baby Aurora to death]] [[EvilIsPetty for a petty reason]], though it is more likely for [[ForTheEvulz the hell of it]]. All the heroes could do was stall for time, which only worked because her minions [[SurroundedByIdiots thought babies stayed the same age]], but once she sends her raven, Aurora is soon ensnared by her powers and is put into a deep sleep by a spindle created by Maleficent. She captures the one prince that could undo the spell and have him wait for a hundred years so Aurora would go mad from the sight of an aged and broken man, implying that even with the counter-curse to her death sentence, she could twist it to something worse. And since she is easily a RealityWarper who was so beyond the heroes in power, that the fairies had to cheat along the way in order to even do her in. She also [[OneWingedAngel turns into a scary dragon with power to match]] that almost manages to defeat the prince, "almost" meaning the fairies had to intervene in order to land a deathblow.
31** ''WesternAnimation/{{The Hunchback of Notre Dame|Disney}}'': Judge Claude Frollo for the entire Disney Animated Canon, for being essentially ANaziByAnyOtherName (and [[PsychopathicManchild a relatively childish one]] at that). In the first 5 minutes of his movie he kills a woman begging in front of a church for sanctuary, and then nearly drops her baby down a well just for being deformed. He does save the baby, but destroys him over the years with verbal abuse and locks him in a belltower. Later he reveals his plan to wipe out all of Paris's Roma. Then he begins to burn down all of Paris in order to find a Roma woman he has the hots for and lets her choose between sleeping with him or being burned alive.
32** ''WesternAnimation/TheLionKing1994'': Scar. The film begins quite cheerful, but when Scar kills his own brother and takes the power, the film definitely becomes quite dark. He may be this to the whole franchise as he's the first Disney villain to successfully kill a main character.
33** ''WesternAnimation/TheLittleMermaid1989'': Ursula, and for two good reasons, too: one, she's a sea witch who (by borrowing Ariel's voice) disguises herself as "Vanessa" in order to take Eric from Ariel. Two, using Triton's crown and trident, she threatens to rule over the entire ocean and turn all the merfolk into worms. Her minions Flotsam and Jetsam are also much more competent than other Disney henchmen and prove a serious threat themselves.
34*** Morgana from ''WesternAnimation/TheLittleMermaidIIReturnToTheSea'' also counts, as she opens the film by trying to feed a baby to a shark.
35** ''{{WesternAnimation/Mulan}}'': [[BigBad Shan Yu]] and the Huns. Every scene they are in is played very seriously and they are one of the few Disney villains to cause so much destruction in their wake, including invading China's borders and slaying the Chinese armies that stand in their way. One scene that best exemplifies their ruthlessness was during the song "A Girl Worth Fighting For" where the Chinese soldiers were singing a happy song about fighting for the women they love when suddenly, [[MusicalisInterruptus the happy song stops]], as does the entire musical portion of the movie, when they arrived at a village that had just been razed to the ground.
36** ''WesternAnimation/TheJungleBook1967'': Shere Khan. As FauxAffablyEvil as he is, his appearance in the original film stops much of the fun and silly mood and makes things more tense and dark. Even moreso where he's concerned in [[WesternAnimation/TheJungleBook2 the sequel]].
37** ''WesternAnimation/ThePrincessAndTheFrog'' gives us Dr. Facilier, a voodoo witch doctor willing to sacrifice all of New Orleans to pay off his debts to dark voodoo entities. Although he gets in on the light, jazzy theme of the movie with a cool VillainSong, it's still clear that he's selfish, relentless and bad to the bone. That he [[spoiler:murders the comic relief in cold blood on screen]] cements this. And then [[spoiler:there's his FamilyUnfriendlyDeath...]]
38** ''WesternAnimation/{{Pinocchio}}'':
39*** The Coachman runs an [[AmusementParkOfDoom amusement park that magically turns young boys who use the attractions into donkeys]]. The ones that lose their voices are then sold to salt mines and circuses, and the ones who ''can'' still talk... well, they're put in cages, and we don't know what happens to them after that. It seems that none of them are ever human or see their homes again, though. [[KarmaHoudini And he gets away with it, too!]] Which is unique, considering [[TheGoodGuysAlwaysWin every other villain falls to the hero.]]
40*** Though the Coachman is far worse, Stromboli is pretty bad too. Both his and the Coachman's actions can be NightmareFuel.
41** ''WesternAnimation/TheRescuersDownUnder'': As mean and dislikable as Madame Medusa was in the first film, Percival C. [=McLeach=], a poacher who sadistically enjoys torturing and killing animals and is more than willing to drop an innocent boy into a river full of crocodiles, proved to be far worse.
42** ''WesternAnimation/TreasurePlanet'': Scroop is definitely the darkest character in the movie, with a threatening appearance and voice to boot. He heartlessly kills [[spoiler:Mr. Arrow]] and is never played for laughs. His evilness stems from the fact that we need someone to root against when [[spoiler:John Silver reforms]], and it's done well.
43** ''WesternAnimation/OliverAndCompany'' is a very lighthearted movie, featuring talking cats and dogs. However, the storyline is a loose SettingUpdate of ''Literature/OliverTwist'', and its human villain Sykes -- the counterpart of the novel's Bill Sykes -- is a LoanShark played utterly straight. There's nothing [[EvilIsCool cool]], [[LaughablyEvil funny]], [[AntiVillain sympathetic]], or even [[EvilIsHammy hammy]] about him. He's just a cold-blooded thug who wants his money ''now'' and doesn't care what he has to do to get it. Hell, even [[WesternAnimation/{{Cinderella}} Lady Tremaine]] and ''especially'' [[WesternAnimation/TheHunchbackOfNotreDameDisney Frollo]], of all people, have a few funny moments and [[EvilIsHammy hamminess]] here and there (and you know, that's saying something).
44** ''WesternAnimation/WreckItRalph'' has the WalkingSpoiler that is [[spoiler:King Candy/Turbo]]. He becomes even more vile during his VillainousBreakdown in the climax, and more sadistic ''still'' after [[spoiler:[[OneWingedAngel getting assimilated by a Cybug]].]] The worst part of all this? [[spoiler:He's ruling over a saccharine world, making him a villain who seems saccharine at first but just gets viler and viler!]]
45** ''WesternAnimation/TheAdventuresOfIchabodAndMrToad'', during the "Ichabod" story. Everything is comedy and laughs, until the ''very'' scary Headless Horseman shows up.
46** Doris the robotic bowler hat and HyperCompetentSidekick of the main villain [[DastardlyWhiplash Bowler Hat Guy]] in ''WesternAnimation/MeetTheRobinsons''. Unlike the laughably incompetent Bowler Hat Guy, she's never PlayedForLaughs, tries to murder Lewis by [[FallingChandelierOfDoom dropping a chandelier on him]], and the movie takes a dark turn when [[spoiler:she betrays Bowler Hat Guy (probably killing him) and creates a BadFuture in her image in which all humans are slaves to bowler hats like herself]].
47** Yokai from ''WesternAnimation/BigHero6'', is surprisingly dark and menacing for a film that's otherwise so lighthearted and adventurous. [[spoiler:Long before he is revealed to be Professor Callaghan, he decides MurderIsTheBestSolution to deal with the titular team (who at that point of the film are just a bunch of powerless "[[ThoseMeddlingKids meddling kids]]" that he personally knows) and when he unmasks himself and gives his MotiveRant to Krei he yells "you took everything from me ([[PapaWolf his daughter]]), so I will take '''[[DisproportionateRetribution everything]]''' from you". In addition, he admits that he doesn't care that the fire he used to steal the microbots and fake his death also killed Hiro's brother, Tadashi.]]
48** ''WesternAnimation/AtlantisTheLostEmpire'': Though its non-musical status and period setting already set it apart from the rest of Disney's repertoire, for the first half-hour or so it's still a fun, mostly harmless pulpy adventure story. Then the Leviathan shows up and blows their sub to smithereens, along with something like 90% of the people on board. From that point onwards, it's straight-up action adventure all the way and with a huge death toll to boot. Commander Lyle Tiberius Rourke is also surprisingly dark for regular Disney fare. He kills a man by punching him in the chest and fully intends to let Atlantis die by taking its power source with the intention of selling it for profit.
49** ''{{WesternAnimation/Cinderella}}'': Lady Tremaine, who always treats our titular heroine like dirt. Ever her daughters Anastasia and Drizella aren't around to give us a good laugh, ripping off Cindy's dress to prevent her from going to the ball, until, of course one of the sisters later TookALevelInKindness.
50** ''{{WesternAnimation/Bambi}}'': Man. The moment the ominous music hits the screen, every animal panics and flees, with the intensifying music punctuated by the sound of a gunshot. When that happens, something dies, including Bambi's mother. And to make matters worse, we actually never get to see what he looks like, but his hunting dogs that were seen look downright demonic. The hunter's presence is almost something out of Hitchcock in a family feature.
51** ''{{WesternAnimation/Tarzan}}'': Sabor delivered the fastest onscreen death in any Disney film; in less than 3 minutes, she killed Kerchak and Kala's baby gorilla. Not long after, she offs Tarzan's parents with a fairly graphic aftermath. She is far more sinister than your typical predator, as she scared the baby gorilla first with a roar, and left the corpses mostly intact, and displays unnerving expressions of glee or rage, making her less of a mindless force of nature and more of a sadistic villain. Unlike Clayton, she has absolutely no amusing moments; Kala and Tarzan are in very real danger of dying, as she proves to be capable of nearly killing Kerchak in a straight up fight. Even as an adult, Tarzan had to fight for his life, and no other villain in the films or TV series were as effective as she was.
52** ''WesternAnimation/{{Frozen|2013}}'' has [[spoiler:Hans]], one of the most chilling depictions of a [[TheSociopath sociopath]] in any Disney movie. He ''is the embodiment'' of VillainWithGoodPublicity [[BaitTheDog even towards]] the viewer. He appears to be a classic PrinceCharming acting out of love towards Anna, but nobody realizes he's actually plotting to usurp the Arendellian throne until it's almost too late.
53*** [[WesternAnimation/FrozenII The sequel]] has [[spoiler:King Runeard, whose campaign to weaken a magical tribe triggered a chain of events that led to the near destruction of his own kingdom.]]
54* ''WesternAnimation/FernGullyTheLastRainforest'' takes place in a forest filled with fairies and wildlife and copious amounts of [[SceneryPorn scenery porn.]] The main villain is [[Creator/TimCurry Hexxus]], the spirit of destruction who first takes the form of a smoke monster and later looks like [[OneWingedAngel a demon straight from hell.]]
55* ''WesternAnimation/HeyArnold'':
56** ''WesternAnimation/HeyArnoldTheMovie'': Alphonse Perrier du von Scheck is the descendant of a commanding redcoat during the Revolutionary War. As a land developer, Scheck has the power and influence to have the neighborhood where a significant battle occurred that the colonists won against his family demolished so that he can put up a mall complex on it and thus reclaim it in the name of his family. He also seeks to have Arnold and Gerald locked up when they get too close to exposing that he's covering it all up, and later on when he's defeated and about to be [[PutOnAPrisonBus arrested]], attempts to run them, and the rest of the angry mob, over with his car[[spoiler:, only to find his [[VehicularSabotage tires were stripped]]]]. And this was a movie to a show that involved ordinary (and occasionally strange) things only happening in this city.
57** ''WesternAnimation/HeyArnoldTheJungleMovie'': Lasombra is a greedy river pirate who abducts Arnold and his entire class, lets his own men get killed in booby traps and nearly leaves Helga and Gerald to fall off the edge of a cliff. He does it all in the name of achieving the Green Eyes' gold artifact the Corazon. He takes immense glee in being evil and is willing to threaten or dispose of any threat in the way of getting what he wants. Once he sees only the children in the tribe are unaffected by the sleeping sickness, he plans to steal all their treasure, leave the adults all in eternal rest and when the Corazon falls off a cliff, he violently picks Arnold up and tries to throw him off said cliff.
58* ''WesternAnimation/HowToTrainYourDragon2010'' has the Red Death. While most of the film is a heartwarming spectacle about Hiccup befriending Toothless and learning that dragons are not truly evil, this character shows up and immediately takes the film in a darker direction, since it explains why the dragons are constantly attacking the Viking village, drives a wedge between Hiccup and his father Stoick, and comes very close to killing a lot of Viking warriors.
59** ''WesternAnimation/HowToTrainYourDragon2'' has Drago Bludivist. Unlike the Red Death, Drago is a human being who is fully aware of his actions, and manages to rapidly push the film into Darker and Edgier territory, even taking the Big Bad of the previous film into account. His introduction via flashback involves him burning down a hall full of Viking chiefs and killing them, with only Stoick surviving. Two major characters die, one on screen, as a result of his actions. Nothing about him is remotely funny, even his Large Ham tendencies, and the mere mention of his name causes the goofy, heartwarming side of things to stop more or less instantly.
60** [[RuleOfThree And since third time's a charm]], ''WesternAnimation/HowToTrainYourDragonTheHiddenWorld'' brings us Grimmel the Grisly. Despite the events of previous installment, the movie starts quite light-hearted, but when he appears, it becomes ''much'' darker. The fact that [[spoiler: Night Furies are almost extinct? You know, about the only dragon species having TheDreaded status among the Vikings in the first movie?]] He's the reason. He also drugs the dragons into obedience ''by their own venom'', is not above backstabing his former contractors, and is very smart, able to outwit Hiccup multiple times. He also likes to give SadisticChoice and using HoneyTrap in his plans.
61* ''WesternAnimation/IceAge'':
62** The [[WesternAnimation/IceAge1 first movie]] is one of the forerunners of the family-friendly CGI comedies. Its villain is Soto, a sabertooth tiger who wants to ''murder a human baby.''
63** Cretaceous and Maelstrom from ''[[WesternAnimation/IceAgeTheMeltdown The Meltdown]]'' are a pair of aquatic reptiles freshly sprung from their icy prison and ready to hunt down whatever creature they find particularly tasty. All the little details surrounding them, from the very visible spite in their eyes when they find Manny and company again to their ScareChord heavy theme, give them a vibe far more in line with a slasher flick villain than that of a silly animated movie's.
64** Rudy from ''[[WesternAnimation/IceAgeDawnOfTheDinosaurs Dawn Of The Dinosaurs]]'' is a towering, stark white ''Baryonyx'' whose roar alone terrifies even Mommy T-Rex and is the one who clawed out Buck's eye prior to the events of the film. As if his physical presence wasn't monstrous enough, he's smart enough to not only remember who Buck is, but hold a grudge over the fact that he's down a tooth because of him. If Cretaceous and Maelstrom were designed like slasher villains, then Rudy is pretty much a prehistoric Kaiju.
65** [[WesternAnimation/IceAgeContinentalDrift The fourth movie]] has Captain Gutt, an evil pirate ape with jagged sharp claws for um...[[MeaningfulName gutting]] his victims. He has a [[EvilHasABadSenseOfHumor sick and twisted sense of humor]]. And he holds a grudge against Manny, a grudge ''so powerful'' that Gutt [[MoralEventHorizon eventually resorts to trying to]] [[spoiler:kill Manny's family just to get back at him.]]
66-->'''Manny''': Alright, let them go!\
67'''Gutt''' (''[[EvilLaugh chuckles darkly]]''): I don't think so. You destroyed ''everything I had''! I'm just ''returning the favor.''
68* ''[[WesternAnimation/KatyLaOruga Katy the Caterpillar]]'' is a sweet little cartoon about a caterpillar exploring the world and meeting wacky characters. Its sequel, ''Katy Meets the Aliens'', focuses on her children doing the same thing--except it throws in a sinister alien who can [[VoluntaryShapeshifting turn into anything it sees]], and zaps the cute little animals away to a prison where it will drain their energy.
69* ''WesternAnimation/KungFuPanda2'' gives us Shen, an AxCrazy [[AristocratsAreEvil banished prince]] who's hellbent on conquering China at all costs. Though he is relatively [[LaughablyEvil funny]], he's ultimately the villain who comes the closest to killing Po, thanks to the cannons he invented from his parents' fireworks, [[spoiler: kills many characters throughout the film, one of them on-screen]], and outright commits genocide in the opening sequence of the movie.
70* The Sharptooth from the original ''WesternAnimation/TheLandBeforeTime''. Beyond simply being outright terrifying, he causes the movie to get much darker after he appears when Littlefoot's mother dies from the injuries he inflicted on her.
71* Lord Business from ''WesternAnimation/TheLEGOMovie'', while [[LaughablyEvil played for laughs at times]], is a serious villain with powerful scope and endless resources. His plan is essentially to freeze the entire Lego universe in order to achieve "absolute perfection", he erases Good Cop's personality and forces him to demonstrate his superweapon, the Kragle, on his parents, all while sporting a PsychoticSmirk, as well as abandoning him in the think tank along with the heroes when [[YouHaveOutlivedYourUsefulness he no longer needs him]]. He also personally kills [[BigGood Vitruvius]] on-screen by ''decapitating'' him with a thrown penny. [[spoiler:Then it turns out that he was based upon the father of the child who created the whole world, who has a HeelRealization when he realizes his son views him as the bad guy, and promptly undergoes a HeelFaceTurn that extends to Lord Business as well]].
72* ''Anime/LittleNemoAdventuresInSlumberland'' has the Nightmare King suddenly show up in a world that was just plain SugarBowl till then, ruling over a section of Slumberland known as [[{{Mordor}} Nightmareland]], the place where nightmares come from.
73* ''WesternAnimation/TheLoudHouseMovie'', despite being for the most part on par with the show's lighthearted tone, has the caretaker Morag, who proves to be far darker than previous antagonists from the show since she's more than willing to brainwash a dragon, terrorize a whole town, and try to kill the Loud Family, which mind you, [[WouldHurtAChild includes a baby]], just to have peace and quiet.
74* ''WesternAnimation/Madagascar3EuropesMostWanted'' follows a parade of colorful animals, some escaped form the New York zoo, others part of a circus. The villain, Chantelle [=DuBois=], ostensibly an animal control officer, is out to ''murder'' the protagonist by [[ImplacableMan any means]] [[TheUnfettered necessary]]. Even after the escaped lion in question is safely contained in the zoo, she ''still'' tries to kill him (and an innocent sea lion!) and steal his corpse so she can add it to her collection of trophies.
75* ''WesternAnimation/MyLittlePonyTheMovie2017'' has the Storm King, a [[LaughablyEvil comical]] yet ruthless and insane dictator who has conquered much of the world outside of Equestria -- the Hippogriffs turned themselves into Seaponies and fled to Seaquestria to evade conquer -- and has set his sights on the magic of the Alicorn Princesses to give him the power to control weather and live up to his name. His [[TheDragon field commander]], Tempest Shadow, an embittered unicorn with a broken horn, appears as the [[TheHeavy more direct and serious threat]], having effortlessly disabled three of the four princesses by [[TakenForGranite petrifying]] them with [[{{Magitek}} Obsidian Orbs]], and actively on the hunt for the escaped Twilight Sparkle with her friends, but then, [[spoiler:we learn that [[FreudianExcuse as a filly, she lost her horn after an Ursa Minor attack, and was shunned by her friends due to her unstable magic sparks]], [[CynicismCatalyst making her believe that friendship is meaningless.]] Later, at TheClimax of the film, after the Storm King finally takes the magic of the Alicorn Princesses, then creates a huge tornado that engulfs all of Canterlot, Tempest asks him to restore her horn to fulfill his end of the bargain, but he backs out of the deal, [[YouHaveOutlivedYourUsefulness reveals that he only used her and attempts to kill her]]. But Twilight [[SaveTheVillain saves her]], leading to her HeelFaceTurn in [[HeroicSacrifice risking her life to defeat the Storm King once and for all.]]]]
76* Oogie Boogie from ''WesternAnimation/TheNightmareBeforeChristmas''. The movie ''is'' dark, the residents of Halloween town scare people because [[PunchClockVillain that's what Halloween is about]]. None of them are SerialKiller cannibals and the movie gets darker when Oogie Boogie tries to murder both Santa Claus and Sally by dipping them in his snake and spider stew.
77* ''Film/OsmosisJones'': For the most part, this is a lighthearted parody of BuddyCop movies with copious amounts of ToiletHumour for the kids all [[FantasticVoyagePlot set inside the human body]]. Enter [[OmnicidalManiac Thrax]]. He's portrayed as a mix between a supervillain and an international terrorist who travels between human hosts (which in the context of the movie are self-sufficient city/nations for countless micro-organisms) and [[TheEndOfTheWorldAsWeKnowIt destroys them]], for no other reason than fame (if he can kill a human in less than 48 hours he'll get a chapter in every major medical text). Also, the [[TouchOfDeath slightest touch from his claw]] is enough to kill other microbes in a spectacular and horrible fashion, [[KillItWithFire burning them from the inside out until they finally explode]]. Then, just to ratchet up the NightmareFuel even further, at one point he counts out his previous victims; one of them is [[WouldHurtAChild a child who "didn't like to wash her hands."]] It says something when the inspiration of the villain comes from "Literature/TheMasqueOfTheRedDeath".
78* [[spoiler:Agatha Prenderghast]] from ''WesternAnimation/ParaNorman''. After her reveal, the movie turns from a fairly lighthearted BlackComedy to a much darker, more serious and more dramatic film, which is even acknowledged in the director's commentary.
79* Creator/{{Pixar}}: Despite the bright colors, the comedy, and the cheerful nature of Pixar, they actually have some very dark and malicious villains that are straight-up evil. Obviously, this type of villain contrasts absolutely with the cheerful nature of the movies.
80** Hopper in ''WesternAnimation/ABugsLife'' is a ruthless tyrant who delights in the fear he instills in the ants, and was fully prepared to publically execute their queen to keep them compliant. He even admits to his minions that they don't even ''need'' the food the ants provide, implying his actions are motivated purely by sadism. His henchman Thumper, on the other hand, is even worse.
81** ''WesternAnimation/FindingNemo'': The film is filled with a colorful cast of characters—virtually all of the villains are either mindless predators, [[ObliviouslyEvil oblivious to their own wrongdoing]], or turn out to be not so bad after all. However, there is a dead-set example in the first five minutes, in the shape of the barracuda that kills Coral and every one of Marlin's children except Nemo. It appears for only a minute, but that minute is often regarded as the darkest in the movie. The mood instantly darkens when it appears, it's one of the few creatures not anthropomorphized, and the rest of the scene is just heart-breaking. It's in effect for the rest of the movie, too; the barracuda triggers a lifetime of mental trauma for Marlin and leads to his violent overprotection, driving Nemo to abandon Marlin and get captured as a result. In a sense, this thing is responsible for the entire plot and all the dark and sad moments within. Not bad for one scene.
82** [[spoiler:Charles Muntz]] in ''WesternAnimation/{{Up}}'', though [[spoiler:Carl's childhood hero]] at one point, is a delusional and sociopathic murderer who kills anyone who he even ''thinks'' [[spoiler:threatens his discovery]].
83** ''Franchise/ToyStory'' is a lighthearted series where the main conflict is usually within the heroes as opposed to external. Villains tend to be either ObliviouslyEvil or relatively harmless. Until ''WesternAnimation/ToyStory3'', that is, where we meet [[spoiler:Lotso, a sadistic teddy bear overlord of a day care center who subjects new toys to being broken by toddlers, tortures, brainwashes, imprisons, and attempts to murder the heroes, and when they save him, he [[UngratefulBastard repays them]] by ''leaving them to die in an incinerator'']].
84** ''WesternAnimation/Cars2'': While the villain of the first Cars film is an arrogant and obnoxious green racecar, the sequel's villains are an organization of evil, beaten-up cars led by a German microcar [[spoiler:and a malfunctioning British SUV]] who commit multiple on-screen murders, torture, sabotage, and attempt terrorism.
85** ''WesternAnimation/{{Brave}}'' gives us a sweet mother-daughter bonding story...with a villain, Mor'du, that happens to be a red-eyed, twelve-foot-tall bear with a taste for human flesh. As well as plenty of scenes that could have come right out of a horror movie, such as [[spoiler:Mor'du watching a young Merida in the forest, Merida going into a castle and having Mor'du sneak up behind her after she's learned his gruesome origin story, and the end fight, where absolutely nothing hurts him except a bear of similar size and a multiton rock]].
86** ''WesternAnimation/{{Coco}}'' takes place in the vibrant, party-filled, music-laden Land of the Dead, which is populated with fondly remembered skeletons and colorful alebrijes. At first, the conflict seems to be that Miguel’s passion for music conflicts with his family's ban on music and he needs to resolve those two in order to get home. [[spoiler:However, we discover that Miguel's hero and best chance to get home, Ernesto de la Cruz, murdered his homesick songwriter partner simply to get famous off the songs he wrote. He then goes on to try and murder Miguel because HeKnowsTooMuch which also puts Miguel's friend Héctor at risk of being DeaderThanDead.]]
87* Rattlesnake Jake from ''WesternAnimation/{{Rango}}'', whose appearance instantly stops the funnier bits of the movie. This serpent with a Gatling gun for a tail is considered to be one of the most dangerous outlaws known, and the only reason he’s been avoiding Dirt is due to a large hawk preying on the town. With the hawk dead, though, Jake’s got no more reason to skip town.
88* ''WesternAnimation/RecessSchoolsOut'': Dr. Phillium Benedict, in contrast to the typical jerks the Recess gang deal with, is a straight-up supervillain plotting to send the Earth into a new permanent Ice Age ([[InsaneTrollLogic believing that the countries with snow have the highest test scores and doing so will improve those of the United States]]).
89* ''WesternAnimation/RiseOfTheGuardians'' is an animated adventure film focusing on a team of SantaClaus, TheSandman, the EasterBunny, the ToothFairy, and JackFrost as they bring joy to children. They also fight Pitch, [[HumanoidAbomination the living embodiment of fear and darkness]], who [[NightmareWeaver gives children nightmares]], commands an army of {{Hellish Horse}}s, [[spoiler: kills Sandy with a triumphant EvilLaugh, {{mindrape}}s Jack, and, in the finale, attempts to [[WouldHurtAChild murder a child]].]]
90* ''WesternAnimation/TheRoadToElDorado'' is a wacky, hammy, screwball comedy with two antagonists who are two different flavors of this trope. Tzekel-Khan is about as light-hearted and comedic as the rest of the film, but is a murderer, a traitor, and is into human sacrifice. Then there's Cortes, who isn't particularly active but seems like he came from a completely different movie, as he has the terrifying ability to kill all of the comedy and all the light in every scene he appears in.
91* ''WesternAnimation/ScoobyDooOnZombieIsland'': [[spoiler:The werecats, Simone, Lena, and Jacques]] are some of the most terrifying ''Scooby Doo'' villains ever, having a humongous ([[OffscreenVillainy off-screen]]) body count achieved by [[spoiler: [[YourSoulIsMine sucking men's souls]] to sustain themselves]], trying to do the same to the Scooby Gang and [[spoiler: dying a nightmarish death.]]
92* ''WesternAnimation/TheSpongeBobSquarePantsMovie'': While [[WesternAnimation/SpongeBobSquarePants the series]] is lighthearted with an IneffectualSympatheticVillain, TheMovie introduces Dennis, a somewhat [[LaughablyEvil comedic]] but still surprisingly terrifying hitman hired to kill [=SpongeBob=] and Patrick using sharp spiked boots. Meanwhile, [[HarmlessVillain Plankton]] turns out to be a NotSoHarmlessVillain, framing Mr. Krabs for stealing Neptune's crown and getting him frozen, coming back in anticipation of seeing Mr. Krabs get [[KillItWithFire burned to death]], and proceeds to brainwash and enslave all of Bikini Bottom. And there's the [[HumansAreCthulhu cyclops diver]], who captures sea creatures and ''painfully kills them using the heat of a bright lamp'', then sells the dried-out remains as knick-knacks. (Whether or not he knows the fish are sapient and screaming is left as an exercise to the viewer. Though he does laugh evilly when [=SpongeBob=] and Patrick are running around their fishbowl in a panic, so whether he knows they're sapient or not, [[CruellaToAnimals he's obviously enjoying what he does in a sadistic way]].)
93** [[WesternAnimation/TheSpongebobMovieSpongeOutOfWater The sequel]] has Burger Beard. Despite being more LaughablyEvil than the other examples, he steals a [[ArtifactOfDoom reality-warping book]] and uses it to steal the Krabby Patty Secret Formula and turn Bikini Bottom into a post-apocalyptic hellhole (with no care for its residents), [[OnlyInItForTheMoney for no other reason than to open his own restaurant, using the Krabby Patty recipe to guarantee success.]]
94* ''WesternAnimation/{{Sing}}'' has a more low-key example with the bears that hunt Mike down for most of the movie. The bears are introduced when [[JerkwithaHeartofJerk Mike]] cheats them out of a large sum of money by cheating at poker. They then proceed to hunt Mike down with the intent of either getting their money or outright killing him. [[spoiler: When they find out that Buster has the money in the form of prize money, they barge into the theatre and ''choke'' Mike until Buster opens the chest. Near the end of the movie, The bears are seen on the back of Mike's car... and then Mike is never seen again, implying that they ''killed'' him.]]
95** [[WesternAnimation/Sing2 The sequel]] has another example in Jimmy Crystal, the CEO of the entertainment company hosting Moon's show. He also has a HairTriggerTemper and [[spoiler: tries to throw Buster off a roof. Twice. He becomes [[SanitySlippage increasingly more unstable]] as the movie's final act goes on. He also emotionally abuses his daughter Porsha, calling her a "talentless loser."]]
96* ''WesternAnimation/TeenTitansGoToTheMovies'' is, for the most part, a comedic {{deconstruction}} of [[WesternAnimation/TeenTitansGo its parent show]]. However, the BigBad is Slade, who, despite being portrayed as a LaughablyEvil DeadpanSnarker, can be just as menacing as he was in [[WesternAnimation/TeenTitans the original show]] when TheGlovesComeOff. For one, he retains his expertise in combat, as evidenced by his first battle with the Titans, showing that he was going to ''kill'' them for the [[{{MacGuffin}} Titonium crystal]] he's trying to get. For another, he's also an expert in MindManipulation as shown in his plan to mind control the world that puts a deeper meaning behind the film's importance of the superhero movie genre. Even in the world of ''Teen Titans Go!'', Slade maintains his KnightOfCerebus attitude.
97* The [[Animation/TreasureIsland1988 Soviet adaptation]] of ''Literature/TreasureIsland'' is mostly a DenserAndWackier take on the source material filled with slapstick comedy, where deaths are either left out or are PlayedForLaughs such as being punctured like a balloon. However, almost every time this version of Long John Silver is on screen after he reveals his true colors, the movie becomes more serious and closer to the text. Although he doesn't look very intimidating, his low, gravelly voice befits a pirate of his reputation, and his more AffablyEvil aspects from the book like his fondness for Jim Hawkins and his boisterous personality were removed, seemingly to make the contrast between his cold demeanor versus the comedic tone of the rest of the two-parter more pronounced.
98* Chef from ''WesternAnimation/{{Trolls}}'' is bound and determined to have all the trolls eaten and killed, has very few comedic aspects, and has no redeeming features whatsoever.
99* ''WesternAnimation/WereBackADinosaursStory'' is a cutesy film about dinosaurs being sent to our time to make children happy. Nothing scary about that at all. Well, except for the creepy old MadScientist Professor Screweyes, who runs a CircusOfFear, has children sign a contract in their own blood, and is eaten by birds at the end of the film.

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