History BrotherSisterIncest / WesternAnimation

** A reference to the fact that Milhouse's parents look extremely alike. Aside from Milhouse, they're the only naturally blue-haired characters in the series. (Marge dyes, to maintain the naturally blue hair colour she had in her youth. Although in one episode it was revealed she didn't know she'd gone grey, and her hairdresser had been dyeing her hair back to blue for years, with the smell of the chemicals giving her repeated short-term amnesia over the matter.) The episode "Let's Go Fly a Coot" reveals that Milhouse's parents ''are'' cousins, though it's not specified whether they're first cousins or more distantly related than that.

* ''WesternAnimation/OneHundredAndOneDalmatiansTheSeries'' had Two-Tone and Lucky as love interests. They're siblings, though it's never mentioned, and to make matters worse they're not supposed to be NotBloodSiblings; they were of the original 12 pups. It's possible the show did change their bond or the writers assumed she was adopted.

** It did an incest joke about Donny and Marie and the [[WesternAnimation/{{Superfriends}} Wonder Twins]] (both in the same episode).

* ''WesternAnimation/FamilyGuy'':
** ''Family Guy'' did an incest joke about Donny and Marie and the [[WesternAnimation/{{Superfriends}} Wonder Twins]] (both in the same episode).

* King Lorenzo and Queen Bunny from ''WesternAnimation/Disenchantment'' are married and are siblings.

** "Fresh Heir" reveals that they bathe together. Since previous episodes have shown members of the Griffin family bathing together, this ''could'' be for perfectly innocent reasons... but since they keep it secret from their parents, it probably isn't.

** Also Chief Wiggum and his wife Sarah, who look like each other and share mannerisms and accents. Like the Van Houtens, they also have blue hair.
** Future Rod and Tod Flanders seem to be lovers.

** "Fresh Heir" reveals that they bathe together. Due to Chris' autism, this could be for perfectly innocent reasons... but since they keep it secret from their parents, it probably isn't.

-->'''Farmer''': I don't trust you, you put your seed in my daughter's belly, you're fired!
-->'''Worker''': But Pa, you can't fire me!
-->'''Farmer''': You're lucky you're my brother too or else I'd kill you!

** ''Family Guy'' did an incest joke about Donnie and Marie.

** Kyle suggests that Stan use ThePowerOfLove to stop her, just saying "Shelley, you're my sister and I love you..." the next time she tries to beat him; Kenny then finishes Kyle's sentence with "...and I want to take off your bra.". It doesn't work.

-->'''Lisa:''' Well, Bart's kinda--- NO!

* In ''WesternAnimation/TheTransformers'', Orion Pax and Ariel were originally boyfriend and girlfriend. After they were killed by Megatron, Alpha Trion recreated them both as Optimus Prime and Elita One and the two continued the relationship in their new lives. Their anatomy turned out to be similar enough that Optimus is able to save Elita's life with an energy transfer. There is no outright mention of "incest", but the implications are still there with Elita and Optimus considering Alpha Trion as like a father to them.

* ''WesternAnimation/YoungJustice'': In the episode "Terrors" Icicle, Jr. is Squicked when Superboy and Miss Martian kiss in front of him because they're disguised as the Terror Twins.