Adaptations change things. Otherwise, what's the point? There's a whole index of 'em. Adaptational Gender Identity is when a character's gender alignment changes — cisgender to transgender, transgender to cisgender, either to nonbinary, etc.
This will often come from the creator's own experiences if the actor playing the character or the writer writing the character are trans. Sometimes it's a Trans Audience Interpretation that was made canon, or a character with an Ambiguous Gender or Ambiguous Gender Identity being explicitly made something.
While not a fanfiction trope, it is very common in fanfiction due to the aforementioned Trans Audience Interpretation.
Subtrope to Adaptational Diversity. Compare to Adaptational Sexuality for changes to sexual orientation. Sister Trope to Gender Flip, where an adaptation changes a character's sex without regard to their gender identity, and She's a Man in Japan, where a translation of a work changes a character's sex or gender.
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Examples:
- Attack on Titan: In the manga, Hange Zoe's gender is left ambiguous, with the mangaka giving only non-answers. In the anime, she's unambiguously a woman.
- In the remake manga version of Birdy the Mighty, Birdy is surprised to learn that Christella Revi is female when all of the Space Federation's data stated Revi was male — only to learn that Revi was born male and subjected to a forced case of Two Beings, One Body — that failed, resulting in Revi's current form being female. The original manga and the OVA never wrapped their up stories completely, but evidencenote does suggest those incarnations of Revi were born female.
- Bleach: Berenice Gabrielli's gender is ambiguous in the manga, with their not even having dialogue. In the anime they're written as male, voiced by a male actor and use the masculine boku pronoun.
- Moriarty the Patriot has Irene Adler transition into a man known as James Bond — who isn't transgender in his canon, either.
- Re:Zero: Ferris has an Ambiguous Gender Identity but is suggested as to identify as female. The anime treats Ferris as a male Wholesome Crossdresser.
- Zig-zagged: the anime for Wandering Son depicts Nitori and Yuki as trans women like in the manga, while Makoto is a Camp Gay boy instead of a trans girl who has yet to transition.
- In the main DC comics, both the pre-Crisis Black Canary and her Legacy Character daughter are cisgender. Black Canary in DC Comics Bombshells is based on the second Black Canary and is a trans woman.
- Demon Knights: In Seven Soldiers of Victory (2005), Sir Ystin is a cis Sweet Polly Oliver who eventually becomes Queen Ystina. In this version, everyone thinks that, however much they explain they're not a man or a woman.
- Multiversity: Teen Justice:
- Teekl's Earth-11 counterpart identifies as non-binary, when Klarion's familiar was usually depicted as female and was interpreted as male in Grant Morrison's Seven Soldiers of Victory (2005).
- Jesse Quick's counterpart on Earth-11 is Kid Quick, who identifies as non-binary rather than female.
- In the DC Comics 2021 Christmas Episode one-shot Tis the Season to be Freezin', a story featuring the meta-LBGTQ+ support group Justice League Queer reintroduces Sigrid Nansen to the post-Death Metal universe. Previously presented as an Ambiguously Gay woman called Ice Maiden, they are now nonbinary and go by Glacier.
- When Arcee was added to the cast of The Transformers (IDW) comics, the Cybertronians were a genderless race and an experiment was done by a Mad Scientist to introduce gender into their numbers, forcefully turning Arcee from a genderless (but male-presenting) robot to a Fem Bot. Several retcons later, it is eventually revealed that primitive Cybertronians naturally existed as both males and females, and that Arcee was "forged male" and actually approached that Mad Scientist for his help in transitioning to a body that fit her female spark despite her male chassis. Every other incarnation of Arcee simply has her forged female.
- Wacky Raceland reinterpreted Sergeant Blast, who was a cis man in the original Hanna-Barbera cartoon, as a trans woman.
- Wonder Woman: While not made explicit due to the culture at the time, Wonder Woman (1942) heavily implies Hypnota is a trans man. Every subsequent writer to use the character has called them Hypnotic Woman and removed any traits that could be considered transgender.
- In The X-Files fanfic #TrustNo1, Fox Mulder, who is canonically a man, is now non-binary.
- 'And Thus...' (A MHA / XC2 Crossover): In canon Izuku Midoriya is a cis male. In this story she is a trans female called Izumi.
- Blessed Curse is an Alternate Universe Fic of Ranma ½ revolving around Ranma as a trans woman and how it changes the story.
- Bloom re-imagines Max Caulfield, of the first Life Is Strange, as a trans girl who recently started taking hormones.
- The Breaking of Hyrule series depicts The Legend of Zelda: Ocarina of Time's Zelda as genderfluid.
- Clair de Lune is a My Little Pony: Friendship Is Magic Twilight/Luna fic where Luna, canonically a cis female alicorn, comes out as a trans stallion.
- Towards the end of the Dæmorphing series, Tobias realises they're non-binary and changes their name to Toby. As far as we know, Tobias was cis male throughout the entirety of Animorphs.
- DC Alterverse Project:
- The third Flash is a trans woman named Wendy West rather than the cis man Wally West, though her deadname is Wallace West.
- The second Black Canary, a cis woman in comics, is a trans woman here, following her incarnation from DC Comics Bombshells.
- In the Homestar Runner/Minecraft crossover fic The Depressor, Strong Sad is a trans man. In the original source material, he is a cis male.
- In A Diamond Under Pressure Raquel is revealed to be a transgender man, saying he's Percival's son "now" and not his daughter "anymore".
- Dream of Acedia (Be Your True Mind): Charlene is initially referred to with feminine pronouns like in OMORI; in Chapter 26, they come out as nonbinary and begin using gender-neutral pronouns.
- Earth-27: In the comics Moonrider of the Forever People is a cis male, while here they were assigned female and identify as non-binary.
- Everyday Craziness in Pontypandy: In canon, Ellie Phillips is a woman. Here, Briar Phillips is nonbinary.
- Everything she deserved: Marcy, Sasha, and Anne were all cisgender girls in Amphibia, but they are all trans or nonbinary here:
- Anne is a trans girl.
- Sasha is bigender and uses both he/him and she/her pronouns.
- Marcy is nonbinary and uses they/them pronouns.
- Fate/Grand Order: Ring of Promise:
- Ritsuka Fujimura is genderfluid and uses they/them pronouns. In the game, the player character is either cis male or cis female depending on the player's choice.
- Mordred is a trans male. In Fate/Grand Order, she is listed as female though she is portrayed as having an Ambiguous Gender Identity.
- Feralnette AU: Felix is portrayed as nonbinary, using he/they pronouns. In canon, he is a cisgender male.
- In Mystery Skulls Animated, both of Lewis' adopted parents are assumed to be cisgender due to them having biological children that have traits of both. In the Courage the Cowardly Dog crossover fic Forever, Lewis mentions his father is transgender while trying to convince a pair of ghosts in a homosexual relationship that LGBT people are more accepted now than they were when the two were alive.
- In Freaky Fabulous Pretty Cure, Frankie Stein's gender is ambiguous, with her using both she/her and they/them pronouns (a combination of Generation 1 and Generation 3). The most elaboration is that they are simply "not a girl".
- Friday Night Funkin': B3 Remixed: In canon, Girlfriend is a cisgender female, while Shaya (the mod's iteration of her) is a transgender female. Her being transgender actually factors into the mod's backstory, as her stress and dysphoria over her father wanting a male heir leads to her running away, meeting B3 soon after; with his support, she transitions into a body she's much more comfortable with.
- Friday Night Funkin' D-Sides: Daddy Dearest, a cis man in the original game, is a trans man in this universe. He remains Girlfriend's biological father, as she was conceived through magic.
- In Friday Night Funkin' HD, Girlfriend is nonbinary and uses she/her and they/them pronouns. In the original game, she was cis and just used she/her pronouns.
- In Friday Night Funkin' Logic, Taki is heavily implied to be a cisgender female in stark contrast to her portrayal in her home mod, where she is bigender and uses both he/him and she/her pronouns.
- Formerly Known as Harry Potter? is a Harry Potter Alternate Universe Fic where Harry's abusive aunt and uncle are arrested and she's taken in by Social Services at age four. She transitions into "Lily" and is adopted by the Grangers, being raised as Hermione's sister.
- The Alice's Adventures in Wonderland oneshot go back to yesterday explores Alice, who is usually depicted as a cis girl, growing up as a non-binary person in the 1800s.
- Glitter Force: Into the Glitterverse: While Tsubasa's gender identity is never commented on in his origin series, he is a trans boy here, and in The Kedabory Verse in general. However, under the effects of the Glitter Force, he is forced back into being a cis girl. He is overjoyed when he's freed from the Glitter Force and can be a boy again.
- Guys Being Dudes:
- Arlo's gender isn't elaborated on in canon. He's explicitly stated to be a Transgender man here.
- Spark is still a man, "more or less", but mentions not fully connecting with any label, including "nonbinary".
- Blanche is referred to with neutral pronouns in canon, but their gender identity is left up to interpretation. Here, they're explicitly stated to be nonbinary.
- At the time this fanfic was written, Rhi hadn't gotten a gender confirmation in English, but most sources described them as male. They're nonbinary here.
- Hammerverse: Izuku Midoriya in My Hero Academia is cis male. Here, as a side effect of having his Gender Bender Quirk from a young age, Izuku identifies as non-binary with they/them pronouns.
- The Some Like It Hot fanfic Had to be you depicts Jerry as genderfluid, genuinely enjoying being "Daphne" even though he no longer has to disguise himself, yet still enjoying being a man sometimes too.
- Here by Accident, Staying on Purpose is an AU comic based on The Fairly OddParents!, where Timmy (a presumably cisgender boy in canon) has come out as a trans woman named Chloe and reunites with Trixie in adulthood.
- Holly Potter and the Witching World is an AU Harry Potter story taking place in a "Witching World" where 90% of the population is female and most of the characters who were male in canon have been Gender-flipped and are female here. One rather unique case is Fred Weasley, who was a cisgender boy in canon, but is a transgender girl here.
- Honoka's Bizarre Adventure: Rin Hoshizora is trans female instead of cis female as in canon. Her own arc involves her dealing with feeling that she is not "a real girl" and being a threat to µ's reputation should her being trans come out.
- How Do I Admit I'm Falling Apart is set in a world where Sonic the Hedgehog (who is presumably a cis man in the canonical franchise) was born female and began transitioning between Sonic CD and Sonic 3 & Knuckles. He's inspired by Extreme Gear rider Wave the Swallow, who formerly went by "Wade the Swallow".
- Hunger Games Simulation: In its spinoff RP Another Side, Another Story, Rei, a ghost child, is identified as a transgender girl, unlike their source material where they're identified as a boy.
- Hyrule Castle High is a Legend of Zelda High School AU where Sheik isn't just Zelda disguised as a boy to hide from Ganondorf, but rather a trans boy who chooses the name Sheik.
- I Wanna be the Troll:
- The Guy is a trans girl in this fic, and changes her name to The Girl accordingly.
- Marco Diaz is a trans girl rather than a cis boy, though unlike The Guy/The Girl, she doesn't change her name.
- The Joestar Chronicles: For some JoJo's Bizarre Adventure characters:
- Erina Pendleton, Yukako Yamagishi, and Pannacotta Fugo are all nonbinary with they/them pronouns.
- Okuyasu Nijimura is trans male rather than cis male like in canon.
- In the fanfiction series JoJo's Bizarre Chatfics, Johnny Joestar and Narancia Ghirga, both cis men in canon, are instead openly trans men.
- Junior Officers: There wasn't any evidence that Kwazii was transgender in the canonical series, but in "A Pirate's Tail", he's revealed to have been a girl before he became a guy. This is a reference to the fact that he was a girl in the original books prior to reprints.
- Kedabory's Elmore Chronicles:
- In canon, Richard Watterson is a cisgender male. Here, Daphne Watterson is a transgender female.
- In canon, Steve Small, while having some feminine traits, is presented as unambiguously male. Here, Sybil Small is non-binary and uses they/them pronouns.
- The Loud House:
- In Who You Are, Lynn Loud Jr. (a cisgender tomboy in canon) is a transgender girl trying to do more "girly" things because she's tired of people misgendering her. Lori finds her wearing a blue dress and tells her that not liking girly things doesn't make her any less of a girl and she should just like what she likes.
- The Real Me makes Lynn Jr. a trans girl and Harold McBride (a cis man in canon) a transgender man.
- The Love Club: Elphaba is non-binary rather than a woman. She doesn't have preferred pronouns, so Fiyero defaults to "they/them", Glinda uses "she/her", and Crope uses "he/him".
- Make a Man Out of You is a rewrite of Mulan where Mulan is reinterpreted as a transgender man.
- Magical Metamorphosis is another trans Harry Alternate Universe Fic where Holly Potter (who didn't know she was trans at the time) passes through the girls-only dorm room unaffected, to Ron and Hermione's confusion, kicking off the plot.
- NiGHTS: Quatro Facets of Dreams: In their respective canons, NiGHTS is nonbinary, Frisk is ambiguous but implied to be nonbinary, and Pump is a cis boy. In the fic, NiGHTS and Frisk are demigirls, while Pump is a demiboy.
- In No Cheat Codes Available, Kirito goes from a cis boy to a trans girl.
- In The Panda Chronicles, Tae-young (presumably a cis boy in the original movie) is a trans boy. "Gone Baby Gone" explains that he was thrown out of his home because of it, which led to him becoming part of 4*Town.
- Princess (Laburnum Steelfang) tries to explain The Penguins Of Madagascar's having matriarchal lemurs ruled by a king by depicting Julian as transgender. The plot kicks off with him accidentally being Forced Out of the Closet by zookeepers that call him a "girl".
- The Phoenix, and Other Early Birds: Both Apollo Justice and Phoenix Wright are trans male, with Phoenix having finished transitioning years before the story. In Ace Attorney, both are cis male.
- Pokémon Crossing: In canon, Benedict's male. In Pokémon Crossing, she's a trans girl named Holly.
- Princess of the Corner has written a Tumblr Post that outlines the plot of Miraculous Ladybug with Marinette as a trans boy named Marin and Adrien as a trans girl named Adrienne. In the show, both of them are cis female and cis male respectively.
- Pokémon Reset Bloodlines reveals that Melissa from Pokémon: The Original Series is a trans woman. This is a nod to how her anime counterpart was male in the original Japanese but was dubbed as female.
- The Power of Friendship (And This Gun I Found!) features Seto Kaiba as a trans man and Mai Valentine as a trans woman, while they were both cisgender in the source material.
- RWBY: Scars:
- RWBY stars a cis girl named Ruby. In the rewrite RWBY Scars, Ruby is a trans girl who lived as a boy named "Crimson" for the first twelve years of her life.
- Jaune in canon is a cis boy, but he's a transgender boy in RWBY Scars, which adds onto his Jeanne d'Archétype inspirations. He transitioned upon coming to Beacon. He cut off his ponytail, stole his grandfather's sword, and ran off without telling his family.
- RWBY Scars turns Weiss' younger brother Whitley into her twin sister Whitley. After being dragged back home by their father, Weiss notices that Whitley has transitioned into living as a boy.
- Sailor Moon Abridged turns cisgender Makoto into a trans girl.
- Scarlet Lady: Marc Anciel is portrayed as nonbinary, and uses gender-neutral pronouns. In the show proper, Marc is a somewhat androgynous-looking boy and is referred to with masculine pronouns.
- Venus/Siren of Marvel Comics was always a cis woman. In Sixes and Sevens, her counterpart Anthea reveals that she is a trans woman.
- It's offhandedly depicted in Solarhood that Sonic the Hedgehog (who was presumably a cis man in canon) is trans when he suffers from period cramps.
- Spooky Twin Pride Month Stories are a series of Spooky Month fanfics set during Pride Month, reimaging all of the cast as some manner of LGBT: in particular, Roy, Skid, and Kevin are transgender, female to male, Robert is nonbinary (and realizes it during the events of their focus story), and the Candy Dealer is voidpunk.
- In the The Owl House fanfic There's No One Like You, Luz Noceda is a trans girl, whereas she is cisgendered in the original show. She experiences some harassment over it, especially after the school learns that she's dating Amity.
- Total Trauma:
- Transitions is a series of Doctor Who fanfics exploring Rose coming out as a trans man, eventually receiving hormone injections and taking the name "Ryan Peter Tyler".
- Transit Twilight is a retelling of The Twilight Saga where Bella is a trans boy named "Jack LaFleche".
- Trans Ponies is a comic where the canonically tomboyish Rainbow Dash undergoes a magic procedure that turns him into a stallion.
- In Undertale, Frisk and Chara both have an Ambiguous Gender and are referred to with they/them pronouns, but have no confirmed label. In Inverted Fate, they are both nonbinary, which is stated on disclaimers at the beginning of later video chapters, and Chara talks a few times about how their abusive birth parents wanted to force them into a traditional gender role, which they hated.
- Warriors Rewrite:
- Firestar is changed from a cisgender tom to a transgender tom and transitioned as a kit.
- To explain how he's a tortie tom, Redtail is a transgender tomcat.
- In the Love Live! fic When One Becomes Many, Honoka and Tsubasa are trans women, You is a trans man, Rina is non-binary, and Chika is genderfluid. All of them were cisgender in Love Live.
- The Word of Your Body is an Alternate Universe Fic where Tobias is intersex and the core four Animorphs are different kinds of transgender.
- The Wound's Still Bleeding is a Naruto Alternate Universe Fic that reinterprets Kakashi as a transgender woman.
- You Are Lisa Simpson is a The Simpsons fanfic where Lisa Simpson, in canon a cisgender girl, is a transgender girl. Allison Taylor and Samantha Stankey are also transgender girls in the fic.
- YuyaVision: the side-story Seraph of Synchro has male Kiryu Kyosuke becoming gender-fluid (crafting his female self as "Kallen"), Placido (an android based off the male Aporia) is non-binary and one-shot opponent Grady (again male) is trans.
- The People's Joker is a a parody of the Batman franchise which reimagines the Joker as a trans woman on a journey of self-discovery. Additionally, Jason Todd is a trans man, Poison Ivy is nonbinary, and there is now a fifth-dimensional imp named Mx. Mxyzptlk, instead of "Mr".
- In The Rocky Horror Picture Show: Let's Do the Time Warp Again, Frank N. Furter, who's usually portrayed as a "transvestite", is portrayed by trans actress Laverne Cox, and has her pronouns changed accordingly. Despite this, she still calls herself a "Sweet Transvestite" in her "I Am" Song.
- In West Side Story's original stage version and 1961 film, Anybodys is a tomboyish cisgender girl. In the 2021 remake, he's a trans boy.
- The book Cinder Ella 2016 re-imagines Cinderella as a trans girl who falls in love with a princess.
- The real life Chris Chandler came out as a transgender woman in 2014. In the alternate timeline of Massively Multiplayer, Chandler dies in 2001, and for fairly obvious reasons remains a cisgender man.
- Peter Darling features Peter Pan as an adult trans man coming to terms with his childhood past and his relationship to masculinity.
- "The Girl Who Was A Boy" features the titular "Girl", Fet-Fruners, who's delighted to be transformed into a man at the end. At least one modern retelling runs with the idea Fet-Fruners is a trans man, and alters the pronouns used, etc.
- In The Babysitters Club, Mary Anne is the preferred sitter for a little girl named Jenny, who is four years old and rather on the prissy side but otherwise fairly unremarkable. Mary Anne is her regular sitter because none of the other sitters like her very much. In the Netflix series revamp, Jenny has been changed to a young transgender girl named Bailey, and Mary Anne is her regular sitter because Bailey's mom trusts Mary Anne — whose father is a coworker — more than she would any other sitter, since she quickly understands that Bailey is trans, and stands up for her as well.
- In the Batwoman comics, Wolfspider is a cisgender man. In the 2019 Batwoman series, Wolfspider is genderfluid.
- In Cowboy Bebop (2021), Gren is reimagined as non-binary, portrayed by nonbinary actor Mason Alexander Park. The character's pronouns are he/him in the original Cowboy Bebop anime.
- Doom Patrol (2019): Danny the Street is non-binary in this continuity, when Doom Patrol comics printed prior to the show's production had the sentient street addressed with male pronouns.
- Good Omens (2019): In the book, Pollution is described as a sickly-looking young man. In the show, they are non-binary (and played by an Asian woman), with even God herself referring to Pollution with they/them pronouns.
- Gotham Knights (2023): Cullen Row is reimagined as a trans boy while his original iteration in the comics is presumably a cis boy.
- Yuji from Liar Game is a trans woman. The Live-Action Adaptation Liar Game made her a cis male with a different design, who was originally Disguised in Drag. The Korean adaptation makes her equivalent a cis woman.
- In The Marvelous Land of Oz, Tip is revealed to have been turned into a boy as a baby and Raised as the Opposite Gender. Though she initially doesn't want to turn back into a girl, she shows no discomfort after turning back into Princess Ozma. In Emerald City, Tip doesn't identify as female when he's changed back into his original form, and only reluctantly stays in a female form to assume the role of Princess Ozma. Later on magic lets him shift back and forth between forms at will.
- One of Us is Lying: Janae is a cisgender girl in the book. Here, in Season 2 it's revealed Janae is genderfluid, coming out to their Love Interest Maeve (who's accepting).
- Sherlock Holmes' Mrs. Hudson as portrayed in the Setting Update Elementary is a trans woman.
- In Supergirl (2015), Dreamer is a trans girl, whereas her comic book counterpart, Dream Girl, is a cisgender girl. Later subverted, as Winn Schott accidentally reveals that there is a Dream Girl in the 31st century, and Dreamer is her very distant ancestor. The TV present-day version of Dreamer was then introduced to the main DC comic continuity as a Canon Immigrant.
- The original The Umbrella Academy has Vanya as a cisgender woman. In the TV adaptation though, just like Elliot Page, Viktor is a transgender man.
- The Mechanisms In their album "High Noon over Camelot", the classic Arthurian character Mordred is AFAB, originally given the name Morgause. They've also clearly transitioned, as even his own father doesn't recognize him when they're reunited after being thought dead.
- While the original film has Jerry remain a cis man after the Disguised in Drag plot is revealed, the stage adaptation Some Like It Hot has the character fully embrace the Daphne persona as her real self.
- In the original Monster High continuity, Frankie Stein was a cis girl. In the G3 reboot, they're nonbinary.
- Cookie Run:
- In the LINE version of Cookie Run, Devil Cookie was male. In LINE's successor, OvenBreak, Devil Cookie is instead nonbinary.
- In LINE, Zombie Cookie was male. In OvenBreak, it is instead nonbinary, being referred to with it/its pronouns.
- Zigzagged: in LINE, Angel Cookie, Cinnamon Cookie, Peppermint Cookie, and Snow Sugar Cookie are referred to with it/its pronouns. In OvenBreak, Peppermint Cookie is still nonbinary but had their pronouns changed to they/them, Cinnamon is male, and Angel and Snow Sugar Cookie's genders are left unspecified.
- Cookie Run: Kingdom: When DJ and Sorbet Shark Cookie were introduced in OvenBreak, their genders were unspecified. In Kingdom, they are both explicitly referred to with they/them pronouns.
- In Fire Emblem: Radiant Dawn, Kyza was male, but in Japan their identity was a bit messier due to a frequent use of female speech patterns and pronouns, a trait often associated with Camp Gay stereotypes. In Fire Emblem Heroes, Kyza now goes by gender-neutral pronouns outside of Japan, heavily suggesting they are non-binary.
- Testament from Guilty Gear had been designed to be androgynous from the start, with long black hair, a slender build, and an outfit with a slit dress (and at one point, had a Crossdressing Voice), but was previously identified as a male. In -STRIVE-, this concept was fully realized, with Testament being redesigned to be even more androgynous and officially going by gender-neutral pronouns.
- In Pesterquest, Vriska Serket, Sollux Captor, and Eridan Ampora are all implied to be under the trans umbrella (Vriska being a trans girl, Sollux being a trans boy and Eridan being ambiguous but possibly nonbinary); none of this was hinted at in the original comic and all were assumed to be cis. Sollux, at least, was later "canonized" by Hussie, though it's hard to tell how serious he was being seeing as the canonization was done in a tongue-in-cheek essay involving Eridan idolizing Shrek (yes, really).
- The Space Goofs adventure game Stupid Invaders turns Candy (who in the show is just an effeminate guy) into a trans woman.
- While an Image Song establishes that the Visual Novel version of Steins;Gate's Urushibara Luka is a cis man, but gay, the anime version, especially the English Dub, says that he feels like he should have been born a girl. The two versions tend to be differentiated by referring to the anime version as Ruka (with an R) or Rukako.
- Alienby Comics:
- "Awakening" is a The Legend of Zelda fanfic in which Zelda is Link's post-transition self.
- "In Full Swing" is based around the common headcanon that Spider-Gwen in Spider-Man: Across the Spider-Verse is a trans woman.
- "Ready to Stand" imagines Ariel from The Little Mermaid (1989) as not only wanting to go up to the surface, but also wanting to be a human woman as well.
- The original Dead End animated short had Barney as an Ambiguously Gay cis man, while the DeadEndia webcomic and later Dead End: Paranormal Park cartoon series made him an explicitly gay and transgender boy.
- The Meaty Yogurt novelization makes Jackie's friend and roomate Saffron transgender.
- Yellow Brick Ramble reinterprets Ozma from The Marvelous Land of Oz as a trans girl to explain why Tip was so willing to stay as Ozma for the rest of her life after being transformed back in the original book. It also has the Sawhorse (Named by the Adaptation as Logan) unsure about his/her own gender identity and experimenting to see if any particular gender fits. And finally, the minor character Dashemoff Daily at least claims to be unsure about his gender identity and trying to figure it out also, though it's possible that was just a ploy to get Logan to agree to go on a date.
- FNAFNG:
- Mangle's gender is intentionally left ambiguous in canon, while she is female in the AU.
- Spring Bonnie's gender is not stated, while he is male in the AU.
- Funtime Foxy's gender is not explicitly stated, though the AU explicitly confirms that they are non-binary.
- Carmilla the Series: LaFontaine gets their name from a minor character in the original novel, Mademoiselle De Lafontaine, who's one of Laura's governesses, and an upper-class cisgender woman. In the show, LaFontaine is non-binary, and only a couple years older than Laura, now being one of her dorm's RAs.
- Paint: The After Ever After series reinterprets Mulan as a transgender man.
- The original Halo from Batman and the Outsiders was a cisgender young woman. In the Phantoms season of Young Justice (2010), Halo comes out as non-binary with a preference for they/them pronouns.
- During the time when Face was the mascot of Nick Jr. in the 90s, he was explicitly male and used "he/him" pronouns. In the 2022 reboot Face's Music Party, their gender is more ambiguous and they now use "they/them" pronouns.
- Much like in the live-action film, the 2022 animated adaptation of Monster High has Frankie Stein, originally a cis girl, identify as nonbinary:
"I'm Frankie! Frankie Stein. I'm 15 days old, and my pronouns are they/them."
- She-Ra and the Princesses of Power:
- Double Trouble was portrayed as a cisgender woman in her appearance in a side comic for She-Ra: Princess of Power, but in the reboot Double Trouble is non-binary and uses they/them pronouns.
- Perfuma, who was cisgender in the original, was designed to be read as a transgender woman, as stated by Rae Geiger and supported by ND Stevenson, though this is not touched on within the show.
- The Star Sisters from the prior show are adapted in the reboot as the Star Siblings, with Jewelstar being the sole brother and a trans man.
- Tiny Toons Looniversity:
- While not directly confirmed at this point in time, Dizzy Devil (voiced by a woman) is heavily implied to be non-binary in the series. There are a few instances where they are referred to with they/them pronouns. In the original, Dizzy was a male.
- Buster refers to Tweety Bird as "they" in the first episode. "It's where Tweety first tawt they taw a puddy tat!"
- While still set in the 1990s like the original X-Men: The Animated Series, hence the character not actually adopting the use of "they/them" as pronouns to avoid Anachronism Stew as views on gender identity weren't as developed they are in the 2020s, Word of God is that Morph, depicted as a cisgender man in the comics and original series, is non-binary.