- Designated Villain: The spider gets crushed to death for the mortal sin of being a carnivore.
- Hilarious in Hindsight: David Hedison's character is the only one to die in the film. And yet in real life, he managed to outlive everyone else in the cast.
- Memetic Mutation: Like we even have to say it...
- While the reveal and bizarre sight of the transformed Andre was genuinely frightening to 1950s audiences, it hasn't aged especially well. Since the fly's head mask isn't expressive, and Andre of course can no longer speak, the final stretch of the extended flashback is mostly lot of dramatic staggering and smashing on his part. This was not uncommon in B-movies of the era as any Mystery Science Theater 3000 viewer can attest, but this film has significantly more lavish production values and better acting than most of those, so for it to suddenly take a turn into the sort of hijinks Matinee went on to spoof is unintentionally funny.
- The infamous "HEEEELP MEEEE! HEEEEEEEEELP MEEEEEEEEE!" climax, though others find it Nightmare Fuel. They had to keep reshooting the scene because the actors couldn't stop cracking up. (Appears as a Shout-Out in the remake, when Seth — under completely different circumstances — whimpers "Help me. Please, please help me," and the Narm is gone.)
- Similarly subverted in the 1965 sequel Curse Of The Fly.
- Nightmare Fuel: The fly's cry (from the page quote) as it is trapped in the spider's web and is about to be eaten.
- Retroactive Recognition: The title character is a young Felix Leiter.
- Signature Scene: Take a guess.
YMMV / The Fly (1958)