- Anticlimax Boss: Killtron seems to be a deliberate inversion of this, as he appears at the end of the Kartoon Kombat tournament ready to fight, but instead reveals himself to be a dancer and then blows himself up just because.
- Ensemble Dark Horse: Killtron can be considered one thanks to his being the villain in the YouTube Poop Little Critter's Odd Fishing Trip.
- Memetic Mutation: Several of the songs, as well as some of the dialogue in general. It was a very popular source for YouTube Poops, the Whinefeld scene especially.
- Nightmare Fuel: The ending, in which the player ends up being trapped in Toonland and becomes a toon forever.
- There's a mini-game in the western room where you can shave Fiddle. All the way down to his muscle, and then skull.
- The grotesque pimple covered character that pops up at random in the hallway. Relief
- Other hallway encounters, such as being confronted by Killtron, count too. Thankfully if you beat Kartoon Kombat, he will not harass you for obvious reasons.
YMMV / Stay Tooned!