- Animation Age Ghetto: Fell victim to this. As a primetime animated drama airing on a network aimed primarily at adults, it had a difficult time attracting a wide audience.
- Cult Classic: Despite only thirteen episodes, the series has a notable fandom in the years since. Neil Patrick Harris was even brought back for Spider-Man: Shattered Dimensions and its more mature and violent look at crime as well as unique art-style makes it a pretty unique take on Spider-Man if not a very faithful or consistent one.
- Hilarious in Hindsight:
- Christina wants to be Spider-Man's girlfriend. Very badly. Tara Strong, who provided her voice, is now playing Mary-Jane in the new Ultimate Spider-Man cartoon. Made even more hilarious considering Mary-Jane isn't Peter's girlfriend in said cartoon. Additionally her attraction to Spider-Man can probably be viewed as a form of Mad Love. Tara Strong would later go on to play Harley Quinn herself, starting in 2011's Batman: Arkham City, with Christina even sporting punk and gothic design elements to her clothing and appearance that Harley would also begin using in that game.
- Peter once says to Harry, "Help me, Harry won Kenobi. You're my only hope." James Arnold Taylor, who voiced Obi-Wan Kenobi in Star Wars: Clone Wars and Star Wars: The Clone Wars, would voice Harry on The Spectacular Spider-Man.
- Peter's design looks a lot like Andrew Garfield, who plays Peter in the Amazing Spider-Man movies years after this was made. It was inspired by the John Romita Sr. redesign which also inspired the casting and look of Garfield.
- In the Latin American Spanish dub, Kingpin is voiced by Juan Carlos Tinoco. This wouldn't be the last time Tinoco voiced a Marvel villain, as he would later become the Latin American Spanish voice actor of Thanos in the Marvel Cinematic Universe.
- Nightmare Fuel: The Lizard himself is full of this. The Gaines Twins, with their malicious behavior and their Mind Rape powers.
- Older Than They Think: Some took note of the Lizard talking in his appearance on the show. While the comics have portrayed him as a savage beast, this wasn't the first time he had ever spoken. His very first appearance in The '60s and his appearances in the Spider-Man (1981) cartoon and Spider-Man: The Animated Series, for example.
- The Scrappy: Lisa Loeb's version of Mary Jane ended up being one of the least popular versions of the character. Not to due to poor acting on Loeb's part, but due to bad writing. Her frustration at Peter wanting to keep her at arm's length makes her come off as whiny and obsessive, especially since she brings it up literally every episode.
- They Changed It, Now It Sucks!: A lot of fans had a dislike of the show merely because most of the villains were Canon Foreigners. Of course, given that a fair amount of these villains were rather uninspired expies of popular canon villains, they may have a point. They also seriously despised MTV's stupid guidelines like not allowing any "old people" on the show, preventing Ensemble Dark Horse J. Jonah Jameson for making too many appearances and Aunt May, one of the most important people in Peter's life and a very iconic and popular member of Spider-Man's supporting cast, from appearing at all.
- They Wasted a Perfectly Good Plot: The show had vast potential to be the best Spider-Man series on the air, due to the fact that it was on a network with fewer censorship guidelines and featured a mature cast and could play with mature themes, not to mention its animation style. What resulted instead was standard MTV fare, largely because of executive constraints.
- What The Hell, Casting Agency?: Keith Carradine as Jonah, trying desperately to emulate J. K. Simmons's take on the character. This bit of casting was seen as particularly strange, as Ed Asner was in the recurring cast as an anti-Spider-Man cop.
YMMV / Spider-Man: The New Animated Series