Oswald Danes, who he makes clear isn't just a The Scrappy to him, he's plain a mistake to include in the first place.
Michael Grade, who let his personal vendetta against Doctor Who tank the show.
Writers and producers have been a frequent target. Rick Berman and Brannon Braga, the former especially in his reviews of Enterprise. Jeri "Pantsuit" Taylor for living vicariously through Cap. Janeway, among other faults. And he'll gleefully savage Maurice Hurley at every opportunity. He's also pretty vocal in his dislike of Kenneth Biller and his inability to tell a coherent story.
The food at McDonald's. Since he mentioned he's still not over that grudge, it's safe to assume it made him badly sick once.
Donald Trump has quickly found his way onto Chuck's list, particularly his "Make America Great Again" baseball caps. Chuck also took a swipe at Trump in the companion video to his "Darmok" review, in 2013, two years before "The Donald" had announced his candidacy.
Similarly, in Dragon Age II, Anders' hypersexual behavior and deeply-inappropriate comments towards a grieving widow results in Chuck disgustedly remarking that the party appears to have been saddled with Harvey Weinstein as a companion.
Also, Anders himself. Out of all the characters in the game, Anders is the only character in the game Chuck can find nothing charitable to say about: many of the team-mates are the targets of exasperated rants throughout the playthrough, but during the big character review in Chapter 8 of Act III, Chuck still finds a few positive or sympathetic traits to talk about. Anders, though, is regarded with nothing but blistering contempt.
Bill Maher gets a shot in the middle of an anecdote about Sybok being inspired by televangelists, describing him as an anti-vaccination fruit loop and scathingly comparing him to an atheist version of the aforementioned televangelists.
Accidental Aesop: In the review for the DS9 episode "In the Cards", Chuck states that the moral went from "Don't waste your money on frivolous things" to "Throw away your life savings on frivolous things" because Jake makes Nog blow his live savings on a baseball card from the 1950s for his father Sisko because Jake can't or won't just replicate a copy and since the Federation doesn't use currency Jake can't get the card in the auction.
In the same episode he summed the episode up by noting that many goods and services exchanged hands during the course of the episode, and for the most part everybody ended up happier, or at least got what they wanted out of it. Maybe that's an idea worth investigating? And if there's nothing you want immideately in exchange for your goods and/or services then maybe you could instead accept some kind of token that represents the value of the service rendered/good exchanged? Then you can use that token later on in exchange for something you want then? Maybe this is something worth investigating?
Adaptation Displacement: The video reviews started life in text format; many of the earliest Opinionated Voyager Guides were taken verbatim from the text-only versions. This helps to explain the Early Installment Weirdness, as they predate all the catch phrases and running gags he introduced later (though he's been shoehorning them in ever since he started re-uploading them).
Anvilicious: When Chuck gets all moral, he lays the speech on thick.
Americans Hate Tingle: Chuck freely admits that he is not an anime fan and really does not like or understand many anime tropes. This all comes to a head in Kannazuki no Miko where he ends up so pissed off at the constantly crying Himeko and Psycho Lesbian Chikane that he makes a point of noting that he is only continuing to watch this show purely because he has been paid to.
His recap of Next Generation events in his Star Trek: Generations review (sung to the tune of the William Tell Overture), which he specifically warns never to bring up again as soon as it's over. Fans are still bringing it up - by requesting the song in audio format. It's widely considered a Moment of Awesome.
After seeing Riker in a coma during "Shades Of Gray", Chuck cuts to... wait for it... a floating Riker head bobbing along to Donuts, Go Nuts! Though, he does claim that earlier in the Review that Jonathan Frakes performance makes it seem like Riker is thinking of doughnuts, so there is set up to it... though, it is still random, Eh?
He pauses mid review of "Profit and Lace" to talk about the wonders of Quaker Oats products.
Broken Base: His hatred of Rob Liefeld. Some say it's justified, others find it incredibly obnoxious and irritating and makes him sound extremely unprofessional.
His version of Janeway, especially after the events of the "Unimatrix Zero" review, where her plan A to fight the Borg involves getting assimilated.
His version of the Warden, Tim the Enchanter, whose goal in life is to find the most creative and hilarious ways of killing people with magic, and is a Blood Knight that would make a Klingon proud.
His Imperial Agent character, a blind sniper utterly failing to cover the fact up who nonetheless proves utterly unstoppable in combat. At one point he accidentally put his scope on backwards and claimed he liked the challenge.
Chuck says in his Batman Beyond review that the movie was censored because it was judged too violent. While that helped, the real catalyst was the Columbine Shooting that occurred before the film came out.
In his review of The X-Files episode "Aubrey", Chuck says Harry Cokley (a suspect in unsolved serial murders from the 1940s) did only 8 years for attempted murder and rape. In the dialogue, however, it's said he was convicted in 1945 and released December 5th, 1993, meaning he must have served 48 years at least.
In the "Encounter at Farpoint" review, while on a rant about actors with foreign accents playing roles of a completely different nationality, he mentions Sean Conneryplaying a Russian. However, Captain Ramius was Lithuanian. In fairness, his nationality in official documents is listed as Russian, at least in the novel, to the point that the Americans are uncertain what his actual nationality is at first (his father pulled some strings, as he felt being listed as Russian would help him get ahead).
Throughout his Star Trek: Voyager reviews, he refers quite disparagingly to the idea that Chakotay has a life debt to Tom Paris from when he saved his life in the pilot, believing it to be stereotypical. In the episode itself, Chakotay makes quite clear that he's the wrong tribe for life debts and just helps Tom out of common decency.
From the reboot, about the death of Spock's mother: "Yo momma so dead, the only thing going down on her now are the worms!"
In the review of the STAS episode "Yesteryear", when talking about putting down fictional animals, Chuck mentions the time when Rainbow Dash broke her leg and had to be put down. Smash-cut to "I can still fly!" BANG!
Describing the Silence from Doctor Who as having the power to make you forget about them the second they're not in your line of sight, "like homeless people."
Dukat and Major Kira's increasingly mean spirited exchange of insults, which culminates in:
Kira: When you go around on your rape sprees, are you worried that youve sired so many bastards, you may accidentally be plowing one of them, or are you just happy that you're finally doing something with your abandoned children? (beat) Again, no offense, Ziyal.
Draco in Leather Pants: Discussed in his Avatar review, where he opines that he doesn't believe Azula deserves much or any of the sympathy fans often give her because although her upbringing explains some of her tendencies, it doesn't excuse them and unlike other characters with similar or worse upbringings that work to overcome their flaws, she does nothing to get over hers.
The "Memory Alpha vs Wookieepedia breast-athon", where Chuck compares the pages on breasts from the Star Trek and Star Wars wikis in order to distract himself from a bad episode, with Star Trek losing both times and Chuck joking that they should step up their game. In Star Trek: Discovery a couple years later, Star Trek did in fact get its first topless shot... in the middle of a rape scene. When he finally got around to reviewing the episode in question, even Chuck noted that the joke wasn't as funny anymore.
In "The Naked Now", he jokes about the Cyrillic lettering on the USS Tsiolkovsky's dedication plaque, specifically claiming that Russia is so poor that they have to use the number 3 instead of a letter. The 3-looking letter is actually the Cyrillic letter Z, but Chuck never mentions this. Then, in a Genius BonusBrick Joke later in the episode, he calls Wesley a "spaz - S, P, A, three, spaz!" In fact, this was so much of a genius bonus that he actually had people trying to correct him in the comments.
In the Old Republic review he insults Hunter as a "scared little girl" hiding from the galaxy. It doesn't come up in his playthrough since he shoots Hunter down at the first opportunity, but this is 100% correct.
Harsher in Hindsight: On the 19th of June 2015, a white supremacist opens fire in a church and kill 9 black Christians. On the 20th, Chuck publishes his review (recorded days before) of "Far Beyond the Stars", expressing that while the situation is better than in the '50s, everything that happened like the Civil Right's movements or the election of Barack Obama do not mean the issue is over, but there is hope.
His final consensus of the Doctor Who story "The Underwater Menace" was that he would not mind if the lost parts of the story (the first, second and fourth episodes) were never found. Later that year, and for the first time since 2005, two episodes of Doctor Who were found... one of which was from "The Underwater Menace".
From First Contact, he sarcastically mentions some of the original ideas for the story, like the Borg in medieval castles, being great ideas... for a Doctor Who episode. A few years later, the episode "Nightmare in Silver" comes out, which depicts Cybermen with abilities very similar to the Borg attacking a castle within an amusement park.
During his review of Mass Effect 2, he made a joke about the Collectors' leader freeing himself from the Reapers' control and preparing to help fighting the Reapers... before the base blows up with him inside. Cue to Mass Effect 3 multiplayer and the Reckoning DLC, which let's you play as Collectors who managed to get free from the Reapers' control.
In "Samaritan Snare", Chuck makes fun of how the doctors seem to be operating on Picard's leg when they're supposed to be fixing his artificial heart, that's not unheard of (the blood vessels in the leg are a great entry point for an endoscope, and heal a lot easier than a chest incision).
In his analysis of the Prime Directive, he talks about a hypothetical nature documentary ending with the narrator saying, "And so the volcano on the island became active, and the entire species will likely die out... and it can't happen soon enough for me, by god! I wanna run them over with a jeep if I thought I'd get away with it!" In the opening scene of Star Trek Into Darkness, the Enterprise crew violates the Prime Directive to save primitive aliens from a volcano.
Mentions an example of this having occurred whilst making a review. Having made Shiva Shepard a blonde to be in line with the officially voted look for default FemShep, he was halfway through his playthrough when the fan backlash lead to a re-election and default FemShep being made officially a redhead instead; at which point he couldn't be bothered to re-record the footage just to make her ginger.
Crazy!Janeway's idea about a device that weaponizes emotions has canonical basis in Star Trek.
His statement that Marvel wanting to release a special edition Blu-Ray of the Howard the Duck film is just one more piece of evidence of Joe Quesada's soullessness, after The Stinger of Guardians of the Galaxy featured a far better received version of the character, which may have been the actual reason they wanted to do it.
The description of individual Daleks as Stephen Hawking if he was a supervillain becomes funnier after seeing The Theory of Everything, in which Stephen Hawking uses his new voice to chant "EXTERMINATE".
In his Gag SubAstromech Spy, R2-D2 is portrayed as a Snark Knight who doesn't get along with anyone, while Chewbacca is surprisingly intelligent. A year later Star Wars Rebels came out, which featured among its main cast a snarky astromech who doesn't get along with anyone based on R2-D2's original concept art and a Genius Bruiser based on Chewbacca's original concept art.
In some of his Clone Wars Reviews, he questions what happens to Ahsoka and Captain Rex after the Clone Wars; and then Season Two of Star Wars Rebels airs, where they appear on a regular basis. And as of the epilogue to the Grand Finale, Chuck's prediction that Vader would kill her becomes even more ironic as it's revealed that she outlived him.
He jokes about how improbable Darth Maul's survival was in his review of the "Nightsisters" arc. By the time he gets to actually reviewing the Maul episodes, years later, Maul had been Killed Off for Real in Rebels.
In his review of Star Wars: The Clone Wars' Geonosis arc, he jokes that now that the franchise is owned by Disney, the Geonosians, a vicious race of termite-like creatures with massive catacombs and spires, will be made more family friendly by Disney, with a song about digging from The Lion King 1½. When a Geonosian actually appears in Rebels, it is depicted in a very sympathetic light... although the plot of the episode is about as family unfriendly as it gets (it's the last survivor of a successful genocide).
At the end of "Wiped, Junked, But Not Forgotten," Chuck admits how unlikely it would be that any new missing episodes of Doctor Who would be found, but if it happened, it probably would be through private film collectors. Regardless, he then ends by noting that Phil Morris and Paul Vanezis are searching African TV stations for any missing episodes, and plays Toto's "Africa" over the end credits. That was in 2011. Two years later, "The Enemy of the World" and nearly all of "The Web of Fear" were recovered... by Phil Morris at a transmitter station in Nigeria.
In his review of Star Trek The Next Generation episode "The First Duty", after Chuck plays Picard's speech to Wesley about The First Duty of a Starfleet Officer, Chuck then has Wesley make poop jokes, joking about "The First Doo-Dee" and calling Commander Riker "Number Two". Patrick Stewart would later play "Poop", a talking poo emoji, in The Emoji Movie.
So Bad, It's Good: Similarly like Ho Yay above: Not the reviews, but it's sometimes discussed for the shows he analyses.
They Changed It, Now It Sucks!: A minor example with the Voyager "Threshold" review. In the original video, some of the lines were made funnier because of the delivery ("He's Dying" and "We Are Not Pokemon!" for example). In the updated Blip version, the delivery was different being harsher in tone than before. However, considering how bad "Threshold" is, anyone would be crankier having to deal with it again.
Chuck has called the show a "political Rorschach test" because of the strange tendency for people from all political alignments constantly accusing him of spreading propaganda for their opponents' ideas. Often from the same joke.
Good luck figuring out his actual politics. For instance, he clearly considers both "people with sexist attitudes" and "feminists" Acceptable Targets.
Whatever his personal political beliefs, it's pretty clear that one of Chuck's core values is contempt for anyone who hews to an ideology - whatever it is - to the point of refusing to consider viewpoints other than their own. When he takes a character or episode representing a social or political opinion to task, it's nearly always for being uncompromising or sanctimonious about its belief or strawmanning opposing beliefs, rather than the ethos being expressed itself.