- Angst? What Angst?: That Angela "Angie" Ashford is nowhere to be seen and nobody ever mentions her is weird, but if we assume the novelization's stance that she died between the events of this and the previous film, then it is jarring that neither Alice nor Carlos seem distraught about it.
- Ass Pull: See the series' page.
- Awesome Music: For the first trailer, The Crystal Method's "I Know It's You", which included vocals by Milla Jovovich herself.
- Complete Monster: The first clone of Dr. Alexander Isaacs, while created to serve Umbrella, was too vile and egotistical to control. Isaacs experimented on clones of Alice, putting them through sadistic tests that resulted in 87 of them dying. He injects zombies with the blood of the Alice clones, resulting in them becoming stronger and attacking him and his fellow scientists, who Isaacs leaves to die. Hoping that the real Alice's blood will domesticate the zombies, Isaacs attacks Claire Redfield's convoy with enhanced zombies in order to kill Alice, killing many people including Carlos Olivera. After being bitten by an enhanced zombie, Isaacs mutated into a monster and slaughtered everyone in his facility, betraying Umbrella for his own dark ambitions.
- Umbrella's board conference has the notable bloop that all the members have physical glasses of water on the table despite they are holograms. A viewer able to notice this might chuckle at the detail.
- The girl calling herself K-Mart because they encountered her in a K-Mart and she felt like changing her name. While this might have been intended to be taken as some kind of coping mechanism for a probable trauma, it doesn't stop it from being rather silly.
- Tyrant-Isaacs proclaiming to be "the future". Considering that, lethal tentacles and psychic powers aside, he looks seriously diseased by his mutation and can barely move around, it is easy to agree with Alice when she laughs at the boast.
- Older Than They Think: As mentioned in Resident Evil: Apocalypse, the similarities between Alice's arc and the 1991 Dark Warrior OVA doesn't end in the previous film. In the sequel to the OVA, the evil corporation the protagonist fights again is led by a scientist in charge of a Cloning Blues project who receives orders from a holographic board of executives, not to count the corporation's head, a sinister, inexpressive blond-haired man who is even more superhuman than the main character. At some point, the scientist breaks away from the corporation and he and his bioweapons have to be put down by the protagonist, who then follows his path of revenge against the corporation. The scientist even looks a lot like Ian Glenn, and the board scene is so similar to the one from this film that it would be shocking that they are not an intentional Shout-Out.
- Rescued from the Scrappy Heap: L.J. drops his Modern Minstrelsy shtick from the last movie and becomes an actual character rather than a "street flava" joke-dispensing device. Unfortunately, it's only then that he gets killed off.
- Special Effects Failure: The scene of the mutated Isaacs killing Slater through his eyes and mouth has some seriously bad CGI on it, in contrast with most of the rest of the film.
- They Wasted a Perfectly Good Character: Given that there is a teenage girl in the convoy who gets a lot of screentime, the viewer could be forgiven for guessing she's Angie after the Time Skip instead of a new character named K-Mart. Had she really been Angie, many interesting storylines would have been possible thanks to her lingering T-Virus mutation and implied psychic bond with Alice. Instead, Angie is literally forgotten, and unlike Jill she never seen nor mentioned again in the franchise.
- They Wasted a Perfectly Good Plot:
- Unlike film continuity, the novelization explains Angie's absence from the film as having been murdered by Alice under Umbrella's cybernetic mind control. Despite being a plot point that would have produced a great lot of drama and could have been used to give Alice more depth, nothing of this is used in the film proper, as Alice and Carlos are positively cheerful in their reunion and never dedicate a word to their vanished friend.
- The goal of Isaacs's simulations with the Alice clones is oddly never explained: he just says he is using their blood to try to make docile zombies, but that doesn't explain why he needs the clones to overcome the original Hive's obstacles. It's inevitable to think that, if the producers were going to include Cloning Blues and callbacks to the previous installments, they could have created many possible interesting plots instead of just because. This is admittedly exploited in Resident Evil: Retribution, but still very superficially.
- What an Idiot!: See the franchise's page.
- WTH, Casting Agency?: Jason O'Mara was disliked in his short role as Wesker, making fans somewhat relieved when he was be recast in the next installment.
YMMV / Resident Evil: Extinction