Angst? What Angst?: Even though Rudy's parents sent him to a Catholic school to "cure" his orientation, he seems very cheery.
Later comics, especially after his breakup with Rain, start to see his constant cheer break down.
In Chapter 34, both Rudy and Maria act like this after Their father catches Rudy going out as "Ruby" and he finds Maria's texts and calls with her girlfriend Chanel, although they both admit they're just using humor to distract themselves.
Rudy can be this at times. While some people love his impulsiveness and boldness, others find him to be obnoxious, and do not like how he has trouble keeping a secret.
Jessica initially. While nobody objected to her coming into the story and offering to help Rain transition, some people found her personality to be a bit too pushy and outspoken, while others loved that about her.
Drew isn't a particularly popular character, but at prom he caused a split in the fan base when a large amount of fans were cursing him for freaking out about Ky's outfit switch with Rudy, saying he was being selfish for expecting Ky to deal with their dysphoria and staying in a dress. On the other hand, another group was sympathizing with Drew, given that he is not confidant with himself and at risk for bullying, and some said that Ky should have been more considerate of that.
Aiken seems to be at least trying to understand Rain now.
Heather used to be disliked, but then got rescued early on due to learning more about gender issues. Even more so when she tells Jessica that she has CAIS and that she resents Kylie for having feminine body parts but not wanting them.
The Scrappy: Currently, Chase and Emily's mother are the most hated characters in the fan fic so far, with both of them being wholly responsible for all of Emily's issues. Needless to say, fans cheered when a recent page revealed Chase got arrested for attacking someone in a drunken rage.
Unfortunate Implications: After Rain's haircut some readers started to comment that Rain isn't supposed to look like that or that she just isn't Rain anymore. Not only does this potentially add to identity policing that trans people go through, that is exactly what Kellen wanted when she chose to cut Rains hair. The author made a shirt design about this to make it clear to readers that those comments rubbed her the wrong way.
The Woobie: Rain. Any time things go well for her, you cheer. But when things go wrong, you just want to hug the girl.
Jessica can count as well. Her family didn't accept her wish to transition if what little she said about them is true, one boyfriend she thought was the man of her dreams ( Aiken) completely abandoned her after finding out her secret, not even bothering to understand her, she was kicked out of her apartment by said boyfriend, and is still having problems with herself.
Emily also counts. She never knew her dad, her mom travels a lot and clearly doesn't care about her and is emotionally abusive, something Emily even acknowledges out loud, she wanted her mother's attention so bad that she drove her girlfriend away ( Maria) and she was emotionally manipulated into a relationship with a sleazy boyfriend who would constantly cheat on her and would have used her to throw parties to perv on her classmates if she hadn't broken things off, and now that she's pregnant, she may have to drop out of her very strict Catholic school in order to raise the child.
Heartwarming Moments: A bunch between the boy and girl but particularly the ending. The boy leaves his home to wake up and save the girl from that nightmarish world, to thank her for waking him up before he died underneath the Unknown's lifeless body. When he reaches the girl's small house next to the circus, he yells and does whatever he can to wake her up. There's an uncomfortable pause and players will be thinking of the worse... but then the balcony door opens and the girl comes out, awake and glad to see the boy.
Player Punch: When the girl seemingly abandons the boy to die underneath the Unknown's lifeless body but really, she's leaving to wake him up.
Toy Ship: Between the boy and girl, particularly in the ending