- Ear Worm: A lot of the music is like this, especially the theme song. It helps that the music is composed by Andy Bean of Wander over Yonder.
- Hilarious in Hindsight: Captain Dog, the pups' favorite dog hero, is an expy of Krypto The Super Dog, Superman's dog. His voice actor, Patrick Warburton, once voiced Superman on The Adventures of Seinfeld and Superman advertisements. Talk about ironic.
- Memetic Mutation: One of the shelves in Bob's house is the chemical structure for LSD.
- One True Pairing: It's minor, but Bob has gotten a bit of shipping with Chloe's mom, mostly stemming from how she's one of the few characters in the series to frequently interact with him, on top of already being nice to him. For example, "Walking The Bob" has her and Chloe slightly confused about why Bob is acting strangely since he's temporarily blind, but they take it in stride, even in the ending when he mistakenly gives them drum sticks as flowers. "Haunted Howl-o-ween" has the pups determined to get Chloe's costume back, making this the first episode where they help someone other than Bob, Hissy, ARF, or anyone related to them.
- Ship Sinking: Chloe's Dad appears in the episode where Bob built Kiea's doghouse, taking the wind out of the sails for those who ship Bob and Chloe's mom
- Spiritual Successor: The show is pretty much the Disney version of Pound Puppies (2010), due to having a similar plot of dogs going off on their job to help someone. Both shows even have some common crew members.
- Tastes Like Diabetes: The episode "Design A Dog" really lays the cute and fluffy aspect pretty thick. Then again, that's probably the point.
- Word of God: the creator answers fans' questions on twitter. Keira was originally named "Tikka".
- Mondegreen: Keia's voice actress originally thought Rolly was pronounced "Rali" before going to the audition finding about the correct pronnouncqtion.
YMMV / Puppy Dog Pals