Adaptation Displacement: Very few people remember that this film actually originated from test animations by the main character's creator.
Award Snub: Inverted. It was amongst the first nominees for the newly-created "Best Animated Feature" Oscar, against Shrek (which won) and Monsters, Inc.. Hardcore animation fans were offended that something so juvenile would be given such an honor.
Throughout the film, Cindy regularly refers to Jimmy by his first name; this is hilarious in the fact that in the TV series, she rarely does so, preferring to address him as Neutron (or "nerdtron", among other mocking nicknames), and this is lampshaded in the episode "Stranded".
One time, Goobot throws one of his henchmen out of the airlock, which the Junkman threatens to do to Jimmy, Carl and Sheen in his introductory episode.
While using one of his inventions that changes his hair (Robo-Barber), Jimmy ends up with a ponytail ("You go girl!") similar to Cindy's in the TV series.
Inferred Holocaust: No infants and senior citizens were present at the film's climax, which means they were left all alone in the town.... Some might have had older siblings, but can we expect all the kids to account for all the helpless babies?
Iron Woobie: Jimmy. He's constantly being picked on for his small size by other people, but that never stopped him from continuing on with his life and enjoying his small group of friends.
This scene◊ of Sheen's Show & Tell presentation is often edited where Sheen and the UltraLord figure are replaced with different people and objects. Usually used as commentary on overexposure of a product, joke, or current event.
Moral Event Horizon: Goobot crosses it when he reveals the parents of the kids are going to be sacrificed to Poultra, and how it's all Jimmy's fault because of Jimmy's transmission.
Signature Scene: The kids using the amusement park rides as rockets to take off into space.
The Problem with Licensed Games: Almost averted for the PC game. While the game suffers from typical movie-based game flaws such as dated graphics, short playtime and clanky controls, it's actually creative in the way it adapts the original script and the humor is very Jimmy Neutron-like. However, the console game suffers most of these problems.
Uncanny Valley: Averted compared to the tests and series where the frame rate makes the film clear it is animated.
What an Idiot!: When the aliens kidnap all the parents in Retroville, they leave behind nothing more than a very suspicious note informing that the parents have gone on vacation. You'd Expect: For someone, anyone, to pick up on the many, many clues that this is a Contrived Coincidence: the obvious type-face "writing" on the note, the ambiguous "Dear son/daughter" address, the fact that it happened overnight to every single parent with zero foreshadowing, and the fact that it happened to everyone at the exact same time. Instead: Everyone would rather focus on the fact that they no longer have to deal with parents, which is a bit understandable given that they're kids and want to bask in this newfound freedom, but even after the novelty wears off, nobody stops and thinks about how suspicious the whole thing is, not even the boy genius himself. It takes Jimmy listening to a recording Goddard made for him the night before for it to finally click that the letter might be fake, and even then, he has to run a comparison on several handwritten letters to figure that part out.
What Could Have Been: If you go through every pre-release media (possibly including trailers), you'll notice they are from a earlier version of the film.
The most notable is the fact that Jimmy and his friends were all going to go to Retroville and rescue their parents is separate rockets rather than just Jimmy and Carl.