- Accidental Innuendo: Dr. Alton's description of Anne's anatomy is rather... detailed. It can give the impression that Alton had a crush on her, and can make you wonder just how far he went in teaching her to be a woman in every way.
- Big-Lipped Alligator Moment: Every single time Bela Lugosi appears, he is sitting in a chair in a room full of horror-movie props, while giving bizarre monologues with very little relation to the previous scene. During the notorious "pull the string!" moment, there is also Stock Footage of stampeding buffalo superimposed over Lugosi.
- Not to mention the Disney Acid Sequence where the devil ends up raping a woman in a sofa...
- Ending Fatigue: Kind of — in the scene when Glen tells the truth to his girlfriend, the music at the end of the scene gives the impression that this is the end of the movie, but then...Inspector Warren: Is that the end of the story?
Dr. Alton: Not quite. I'll get back to it in a minute.
- Of course, Dr. Alton did say that he was going to tell two stories, but the audience may have forgotten this by this point, or they might assume that this is the end of the first story, even though it isn't.
- Ensemble Dark Horse: The Scientist is without a doubt the most popular character in the film. Ed Wood seems to have predicted this, as his actor was marketed as the star of the film.
- Evil Is Sexy: Satan, while taking the form of Barbara.
- Fair for Its Day: The movie has a fair share of Values Dissonance, though considering when it was made, it was actually very forward-thinking.
- It manages to avert Trans Equals Gay, a trope that is still persistant to this day, but does so in a way that implies that homosexuality isn't "normal," but crossdressing is.
- Glen's story ending with him "curing" his crossdressing comes of as rather ignorant today, but unlike other, similar examples of this trope it is said that this should only be done under certain cicumstances and with the patient's consent. His "condition" is also treated more akin to a drug addiction than a mental illness, and is seen more as a symptom of a problem than a problem in and of itself, which mostly explains the portrayal of crossdressing in the film — that is, the movie argues that crossdressing is usually harmless, but if it is hurting you and/or the people closest to you, you should stop it. Considering that Ed Wood himself continued to crossdress long after making this film, one can also imagine that he would probably have shown Glen continuing to do so if he could, but the political climate of the time (and possibly also the studio) simply wouldn't permit it.
- Anne's story, which has her leave the army and becoming a housewife has some strong Stay in the Kitchen vibes, but it is still a clearly positive portrayal of a transgender woman, and Dr. Alton feels that letting her transition was the best solution to her problems. Considering that the movie was made around the time when the fact that sex changes existed was front page news, this is quite remarkable.
- The portrayal of indigenous people can also come of as rather racist, as they are treated as more "primitive" than the "normal" people in the civilized world. Though while Dr. Alton sees them as less civilized, he doesn't look down on them for that, and actually argues that - in certain ways - they may be more "natural" than people in modern societies have become.
- Fridge Brilliance: From a Meta perspective, it makes sense that the Scientist is so surprised when the erotic scene starts. He is supposedly reading the story from his book, and this part wasn't in Ed Wood's original script. The Scientist understands that he is supposed to be all-knowing, and is shocked to see the plot go Off the Rails for five minutes.
- "Funny Aneurysm" Moment: The ending, where Glen and Barbara - played by Ed Wood and his real-life girlfriend Dolores Fuller - get married and live Happily Ever After becomes this when you consider that Ed and Dolores never got married, and separated a few years later.
- Genius Bonus: The phrase "snails and puppy dog tails" is a phrase from an old English nursery rhyme with titles varying from "What Are Little Girls Made Of?" to "What Little Girls Are Made Of," which humorously talks about the differences in boys and girls. Famously, girls are said to be made of "sugar, spice, and everything nice" note and boys are supposedly made of "slugs, snails and puppy dog tails."
- Ho Yay: Surprisingly little, though there is some of it in Glen's dream.
- One of the things he sees is a Damsel in Distress being rescued... by another woman. There is more Les Yay later - with some I Have You Now, My Pretty undertones - as a woman attacks a lady resting on a couch, tying her up with ropes and a mouth gag.
- Satan transforming into Barbara and seducing/mocking Glenda actually manages to be both Ho Yay and Les Yay at the same time.
- Memetic Mutation: PULL THE STRING!!
- Beware of the big green dragon...
- Narm: This movie is loaded with it.
- Narm Charm: Bela Lugosi's scenes have no connection with the plot, but every single line he delivers is fantastic.
- The scene where the suicide note is read out is actually rather effective, despite the seemingly random shot of a radiator and the fact that you can see that the "corpse" is breathing.
- The speech about the Big Green Dragon actually becomes rather creepy when it is said by Satan, who stares at the viewer and apparentely communicates with them telepathically.
- So Bad, It's Good: In large part thanks to Bela Lugosi's monologues.
- Some Anvils Need to Be Dropped: Crossdressers are often perfectly nice people who deserve to be treated with respect, not looked down upon or laughed at.
- It is better to stay true to who you truly are inside, than to live a lie.
- Took the Bad Film Seriously: Ed Wood, of course. He took what could have been a simple exploitation film and tried to turn it into a great, character-driven story with a plead for acceptance, but he didn't quite have the talent or the budget to really pull it off.
- Vindicated by History: To a certain extent. It is still not seen as a good movie, but it is a least somewhat appreciated for having a very brave message that was ahead of its time.
- Values Dissonance: The trope is Discussed In-Universe when Dr. Alton points out that there were several tribes in the "less civilised parts of the world" where it is seen as perfectly normal for men to make themselves look pretty for their wives. They do not see it as "unmanly" at all. He also points out that cars and airplanes were once seen as weird and threatening, but are now an essential part of today's society.
- Values Resonance: While the film does have some Values Dissonance, some of its messages are still relevant.
- The suicide scene arguably hits harder today than it did back then. The movie portrays Patrick/Patricia as a Justified Criminal who really didn't deserve to die like that, but a modern audience might see him/her more like a victim of discrimination who couldn't take it any more.
- In general, the portrayal of crossdressing is rather nuanced. It is said that people do it for a variety of different reasons, and that they should be judged on a case-by-case basis, as even people who seem very similar can require completely different kinds of help.
- What Do You Mean, It Wasn't Made on Drugs?: Some of the more surreal parts of the film qualify. Nobody is going to accuse you for wondering if Lugosi was reading from a script or was actually high and trying to warn the crew of a dragon he was hallucinating about.
- WTH, Casting Agency?: As Look Back in Angora stated, "Only Ed Wood would have cast Dracula... as God!"
- Though in fairness, Lugosi does a pretty good job. The problem is that his scenes are utterly bizarre and that his character only seems to exist to give him somebody to play.
YMMV / Glen or Glenda