- Accidental Innuendo: "Wait, are you guys gonna lick me?"
- Awesome Music: Puppycat's song/story in the pilot.
- Base-Breaking Character
- Keith (the Cleavage Crab) is either funny or annoying and promotes sexual harassment.
- Bee. She's either quirky and funny or annoying.
- Broken Base: The decision to release the season finale along with the rest of the show exclusively on VRVnote has split fans, with some fans understanding that Youtube ad revenue really can't pay for high quality animations while others argue that the whole point of the kickstarter was so the show could be made without needing a paid subscription.
- Big-Lipped Alligator Moment: The talking cicada in "Food", which, unlike the other talking animal gags, shows up for one scene and then is literally thrown out by Puppycat.
- Fridge Horror: Wallace's fate raises some scary questions. It seemed Puppycat had met him before, so it's probably safe to assume that the information Puppycat had on him was accurate and that he was in fact an "ordinary" fish previously. He appears to have been possessed, but did he die during the process, or was he alive but unable to do anything until the monster popped out of him? Or was he replaced and is dead/trapped somewhere? What will happen when his mother comes home and finds him gone?
- Alternatively, he may have been a monster all along, and was simply biding his time until Puppycat revealed what he knew about the space outlaw. Mitigates the above Fridge Horror somewhat, but replaces it with a different kind of horror.
- "Donut" brings one up in regards to Bee and the revelation that some parts of her are robotic. If she wasn't born that way...what exactly happened to her?
- Fountain of Memes: Bee is definitely one. See the Funny Moments page.
- Internet Backlash: Fans were not happy when Cartoon Hangover announced that Bee and Puppy cat along with their other shows would be available exclusively on VRV and would no longer be put on Youtube. Not helping matters is that it currently only works in the United States while other countries currently have to wait even longer, and that the show would make audiences wait an extra month more than they would if they subscribed, frustrating fans who had grown tired of the long, 2-year hiatus.
- Moe: Bee, Puppycat and Wallace before his transformation.
- Most Wonderful Sound: Puppycat´s voice. Helps that he's voiced by a Vocaloidnote .
- Squick: Bee biting the monster's tongue, which turns out to be entirely effective, causing blood to squirt everywhere.
- Take That, Audience!: The wishing hole in the finale seems to be a subtle jab at fans who pirate the show. Especially obvious when the bakery employee explains how sometimes his customers give him a gift as payment, while more often they just give him nothing in return. Ultimately the wishing hole goes berserk and tears the entire planet apart, mirroring how fans pirating the later episodes harmed the show in the process.
- They Changed It, Now It Sucks!: Many fans were disappointed by the changes in art style and characterization between the pilot and the actual show. (For example, Bee having her Manchild tendencies turned up.)
- Tear Jerker: The story of the space outlaw and the space princess told by Puppycat. Especially with the implication that it's his backstory.
- The finale of the first series. Cardamom's mother doesn't wake up from her coma (though she does start crying), Deckard goes off to the academy without directly saying goodbye to Bee, and Puppycat and the audience learn Bee is at least partly robotic just in time for the show to end. It isn't all sad, but it does end on a very bittersweet note.
- What an Idiot!: At the end of Donut, Cardamon is given a donut that can grant one wish for the person who eats it.
You'd expect: Cardamon eats the donut and wishes for his comatose mother to come back to life.
Instead: He tests a bit on his dog (which could potentially have wasted the wish) then feeds the whole thing to his mother.
Result: She doesn't wake up; instead, salt water and jellyfish start pouring out of her eyes. Cardamon cries himself to sleep.
- What Do You Mean, It's Not for Kids?: Bee and Puppycat airs on Frederator Studios' Cartoon Hangover, which is an outlet for adult animation, and despite the pretty colors and perky protagonist, Bee and Puppycat isn't targeted towards kids. It is relatively downplayed in that there isn't really much in the show that's objectionable in the first place (especially compared to sister show Bravest Warriors)—with really the only glaring "naughty" stuff present being some mild language, an innuendo here and there, and a brief splash of blood in the final episode—but the recurring theme of settling into adulthood do resonate more seriously with the target demographic.
- What Do You Mean, It Wasn't Made on Drugs?
- The Woobie: Wallace the fish. Not only is he SO SAD(!) but at the end it's implied that he's been killed and possessed by an Eldritch Abomination.
- Iron Woobie: Both of the titular characters.
YMMV / Bee and Puppycat