The film follows the adventures of Huckleberry Hound, who, in his journey to a raise a small farm with goats and pigs, comes upon the California town of Two-Bit, where he meets up with the residents, Yogi Bear and Boo Boo, who've run away from Jellystone Park; Snagglepuss, who serves as the piano player at Rusty Nails' Saloon; Hokey Wolf, who acts as the town's mayor, and Quick Draw McGraw and Baba Looey, who run the Two-Bit National Bank. Unfortunately, Huckleberry runs afoul of a group of bandits known as the Dalton Gang: Hair Trigger Tempered leader Stinky Dalton, diminutive Manchild Finky Dalton, Ambiguously Gay Pinky Dalton, and Dumb Muscle Dinky Dalton. Huck is appointed as the new sheriff of Two-Bit, where it is his job to try to rid the town of the notorious Dalton Brothers.
A subplot of the TV film also includes Huck forming a romantic relationship with an American Indian girl hound named Desert Flower, which occurs after Huck gets blasted off by the Daltons.
The movie was the last production voiced by Daws Butler, who passed away on May 19, 1988 - just days before the movie premiered on television - which added a certain piquancy to the ending, which reveals how the characters wound up. Especially as Huck rides off into the sunset, singing.
- Accidental Misnaming: When Huckleberry rides off on his 'Faithful Horsie' who he discovers can talk;Bob: Huck, would you please stop callin' me 'Faithful Horsie'?Huck: Why?Bob: Because my name is Bob!Huck: Oh.
- Ambiguously Gay: Pinky seems to show shades of this, as he dresses in pink, often complains about Huckleberry Hound being a rotten dresser, and is always complaining about the decor of the Daltons' secret hideout.
- Anvil In His Glove: When Huck fights Finky, Pinky, and Dinky, he wins with the help of 'Betsy', who is this.
- Arson, Murder, and Jaywalking: After Stinky has been captured and put on trial, the Judge lists all of his transgressions:
- Judge: Stinky Dalton, you've been charged with assault, train robbery, cattle rustling, and worst of all, forgetting Mother's Day! (jury gasps)
- Babies Ever After: The ending of the film shows that Huckleberry marries Desert Flower, and not only do they get to raise goats and pigs on a farm, but they also have three children.
- Barefoot Cartoon Animal: Other than the regular characters, there's also Desert Flower and the rest of her tribe.
- Born in the Theatre: During the chase scene, the Daltons ride so fast they end up almost riding out side of the film's frame.Stinky: Hey, not that fast!
- Brick Joke: The blue fountain pen that Huck receives from the bank as a prize gets stolen by the Daltons when they rob the bank. It's not until near the end of the film that Huck gets it back in order to sign a young boy's autograph for capturing the Daltons.Dinky: Ah, rats! He remembered!
- The Cameo: Besides Huck, Yogi, Boo-Boo Snagglepuss, Quick Draw, Baba, and Hokey, other Hanna-Barbera characters also make cameos in the movie. Peter Potamus appears as the captain of the ship bound for Tahiti, Magilla Gorilla appears as a reporter interviewing a Bound and Gagged Mr. Peebles, and Muttley, Doggie Daddy, and Snooper appear as audience members in the scene where Huck is trying to pick a complimentary prize from the bank.
- Curb-Stomp Battle: Even despite Huckleberry's best efforts to win the Test of Strength in the Initiation Test, Chuckling Chipmunk beats him easily.
- Disguised in Drag: When Huck goes looking for help against Stinky, Snagglepuss tries to avoid him by putting on a dress and wig. Huck doesn't realize it until after Snagglepuss scampers away.
- Dumb Muscle: Dinky (similar to his portrayal in Laff-A-Lympicsnote )
- Easy Amnesia: Huck develops this when he is found by Desert Flower, due to the injuries inflicted upon him by the Daltons.
- Epic Fail: After being thrown out of town, the residents try to make a living by operating a carnival. Unfortunately, the customers are so dissatisfied that they demand double their money back.
- Falling-in-Love Montage: Huck and Desert Flower fall in love while Huck stays with her for a while.
- Fluffy the Terrible: Chuckling Chipmunk, despite having a cutesy-sounding name, is actually a huge muscle-bound Laughing Mad warrior of Desert Flower's tribe.
- Frothy Mugs of Water: When Pinky, Finky, and Dinky are served drinks at Rusty Nails' saloon, the implications of alcoholic drinks are removed, only having them drink concoctions such as Rattlesnake Juice and a Shirley Temple with two cherries in it. Also, Huckleberry orders some root beer when he enters the saloon.
- Hartman Hips: Rusty Nails.
- Jail Bake: This was subverted, as while Stinky Dalton (disguised as his brothers' grandmother) offers them a cake, Huckleberry takes it from him, thinking that he hid a file in it. It was actually a distraction so that Stinky could give Pinky, Finky, and Dinky the tools necessary for them to escape from jail while Huck was busy eating the cake and there is no file.
- Laughing Mad: Both Finky Dalton and Chuckling Chipmunk.
- Literal Metaphor: After Huck has been appointed as sheriff of Two-Bit, the narrator explains that Huck's first job as sheriff was cleaning up the town. Cut to Huck, wearing a garbage man's uniformnote , picking up the trash.Huck: I do not do windows.
- Offscreen Teleportation: Huck does this when he chases the Daltons.
- Psychopathic Man Child: Finky qualifies for this, as he's the size of a small child, yet is very cunning and tends to laugh maniacally.
- Real Men Wear Pink: Pinky Dalton.
- Reports of My Death Were Greatly Exaggerated: In the climax, Huck takes advantage of the Daltons' belief they blew him up by possessing as a ghost.
- Running Gag: Pinky calling Huck a rotten dresser, as well as Finky's Malicious Misnaming of Huck.
Huck: Not to mention my spiffy western duds.
- There's also any mention of Huck's faithful horse and fancy saddle.
- The title is an obvious reference to The Good, the Bad and the Ugly.
- When the residents of Two-Bit return from Tahiti to find that the place was renamed to Daltonville, Quick Draw quips that it would qualify for an episode of The Twilight Zone.
- The chase scene has a one to two Droopy shorts in which the Daltons (with Stinky having rejoined them) trying to escape Huck only for Huck to appear wherever they go. This is later subverted when the Daltons realize they outnumber him 4 to 1.
- Spoiler Opening: Of a sort. The commercial bumpers (which were left intact on the DVD release) spoil the aforementioned Babies Ever After ending a mere seven minutes into the movie.
- Suddenly Speaking: Huckleberry's faithful horsie, who had been voiceless up until Huckleberry gets separated from him then finds him, reveals that he can talk. Not to mention that he [the horse] also reveals his name to be Bob.
- They Call Me Mister Tibbs: When Finky refers to Huck as "Huckle-poo".Huck: That's "Sheriff Huckle-poo" to you.
- The Unintelligible: Chuckling Chipmunk, who can only cackle maniacally. Though, he does coherently say, "I win", after beating Huck in the Test of Strength.
- Villain Song: "Gold, Gold, Gold".
- With Friends Like These...: With Stinky coming to town, Huck looks for help from the friends he made in town. Every single one refuses to help and even beats it outta town before Stinky arrives. Huck lampshades the trope when Mayor Hokey has him sign a million dollar life insurance policy (of which Hokey is the sole beneficiary).