Jessica Drew was bitten by a spider when she was a young girl. To save her life, her father gave her a serum that also ended up giving her special powers. As an adult, Jessica fights crime as Spider-Woman, working as the editor of Justice Magazine in her civilian identity.
- Adaptational Heroism: Jessica's father was a HYDRA agent in the comics. In here, he was apparently just a kind scientist.
- Amazon Brigade: A tribe of Amazon women were the antagonists in "The Amazon Adventure".
- Animated Adaptation: A cartoon loosely based on comic book heroine Spider-Woman.
- Darth Vader Clone: Graviton, the villain of "Invasion of the Black Hole", is clearly based on Darth Vader from Star Wars (the series premiered two years after the release of A New Hope). He wears black armor, his face is obscured by a similarly-shaped helmet, and he even fights Spider-Woman with a lightsaber-like weapon.
- Distressed Dude: Jessica's love interest, her nephew, and Spider-man all become the victims so Spider-Woman has to save them in many episodes.
- Fight Dracula: Spider-Woman faces Dracula in the episode "Dracula's Revenge" as well as the Wolfman and Frankenstein's Monster.
- Film Felons: "The Kongo Spider" had a villainous director try to make a snuff film with Spider-Man and Spider-Woman as his victims.
- Idiosyncratic Wipes: Scene transitions often showed a spider web surrounding Spider-Woman's insignia before cutting to the next scene.
- Lighter and Softer: Part of the course for the cartoons of the era. The original run of Spider-Woman was full of drama, with Jessica being an outcast for her strange powers and lack of identification, and she also had to deal with being a former HYDRA agent. Here, she is a stable reporter with a nephew and Love Interest unaware of her identity as a superheroine, and no mention is ever made of her background (So her father, by all intents, is a nice guy in this version)
- Monster Mash: "Dracula's Revenge" had Spider-Woman fight Dracula, the Wolfman, and Frankenstein's Monster, who were all able to transform their victims into their own kind by firing energy blasts at them.
- Mummy: The villains in the series' first episode were a race of alien mummies from the planet Hotep.
- Robot Me: In "The Kingpin Strikes Again", Jessica Drew uses a robot Spider-Woman to convince the Kingpin that he made a mistake in assuming Jessica Drew was Spider-Woman's secret identity.
- Rogues-Gallery Transplant: The episode "Realm of Darkness" had Spider-Woman fight Dormammu, who is traditionally an enemy of Doctor Strange.
- Role Reprisal: While not credited, Spider-Man's appearances in the series had Paul Soles reprise his role as the web-slinger from Spider-Man (1967).
- Rushmore Refacement: In "The Great Magini", the titular villain at one point uses illusions to make it seem like he's stolen the Presidents' heads from Mt. Rushmore and has large duplicates of his own head take their place to boast about his crime.
- Stock Footage: Par for the course of the cartoons of the era.
- Transformation Sequence: In a manner similar to the 1970's Wonder Woman television series, Jessica Drew changes into her alter ego and back by spinning around.
- Villainous Glutton: The Kingpin is constantly seen eating something and is a cunning crime boss.