Unlike its predecessor, Octonauts: Above & Beyond is focused on land creatures. It also adds Octo-Agents, whom are friends/family of the Octonauts who assist with missions.
- Action Girl: Dashi takes on a brave, leader-type role in many of the missions.
- Action Mom: Pearl, who is a brave, intelligent Octo-Agent as well as a mother.
- Ascended Extra:
- Some minor characters from the original series, such as Calico Jack, Pearl, Ryla, Ranger Marsh, and Professor Natquik, have been promoted to Octo-Agents.
- Dashi has a much more major role in this series, going from one of the lesser supporting characters to such a major character that she almost outshines Captain Barnacles.
- Canon Foreigner: Paani, a monkey from India who is one of the Octo-Agents, was introduced in this spinoff.
- Episode Tagline: "Keep a steady paw" for "The Wild Windstorms".
- Faint in Shock: Tunip does this in the cocoa episode, when he realizes there are no kelp cakes.
- Furry Confusion: Polar bears are anthropomorphized, but panda bears are not.
- No Full Name Given: Paani's surname is not known.
- Out of Focus: Captain Barnacles and especially Kwazii have a much smaller presence in this series compared to the original.
- Pandaing to the Audience: "The Bamboo Rescue" is about panda bears.
- Playful Otter: Periwinkle, a Cheerful Child otter pup.
- Plot-Relevant Age-Up: Periwinkle, who was a newborn pup in the original series, is now a child who attends Otter Scouts while Pearl works as an Octo-Agent.
- P.O.V. Boy, Poster Girl: Gender-inverted: Captain Barnacles is featured heavily in marketing, but Dashi has the largest presence in the series.
- Sleepwalking: "The Sleepwalking Dormice", which is about exactly what the title implies.
- Vocal Evolution: In US prints, Pinto no longer has a Mexican accent.
- Wrap-Up Song: "Octo-Report", a sort-of sequel to the original series' Creature Report.