In this animated short, Sylvester is chasing after Tweety as he always does, when he is interrupted by an unnamed cat (later retconned into "Clarence" in Looney Tunes comics) who brings him to a group called "Birds Anonymous" which consists of cats trying to kick their bird habit. Sylvester pledges sobriety, stating that "birds are for the birds," but falls off the wagon and chases after Tweety again. His sponsor tells him that cats and birds can live together in harmony, and kisses Tweety to prove it — whereupon the sponsor also breaks down and tries to eat Tweety. This time Sylvester intervenes, and the short ends with Tweety saying "Like I said before: Once a bad ol' puddy tat, always a bad ol' puddy tat!"
Winner of the Academy Award for Best Animated Short Film, it is also included in The 100 Greatest Looney Tunes, amazingly the only cartoon to hold both distinctions. Widely regarded as Friz Freleng's greatest cartoon, Mel Blanc also felt that this was his finest acting performance.
- Big Damn Heroes: Sylvester's sponsor stops him from eating Tweety by filling his mouth with alum (shrinking his mouth as a result).
- Bring the Anchor Along: Sylvester handcuffs himself to a radiator to keep himself from getting Tweety. But when he can't restrain his urges any longer, he just charges at Tweety, yanking the radiator off the wall.
- Broken Tears: When Sylvester finally admits he has an addiction.Sylvester: I can't stand it! I gotta have a bird! I'm weak! I'm weak, but I don't care! I can't help it! After all, I am a pussycat!
- Cold Turkeys Are Everywhere: Poor Sylvester just can't catch a break.
- Darker and Edgier: Relatively speaking, the subject here is significantly darker than most Looney Tunes cartoons, especially those made by Friz Freleng, for portraying addiction mostly sincerely. It was considered revolutionary at the time for a cartoon to address such a real-life issue.
- Didn't See That Coming: At the end, Sylvester actually seems to be able to stay on the wagon (at least for now); his sponsor does not.
- Drugs Are Bad: At the BA meeting various cats tell stories of their bird addictions.
- Fantastic Drug: A cat's desire to eat birds is treated like this, withdrawal symptoms included.
- Growling Gut: Sylvester's roaring stomach is just one of the many things trying to throw him off the wagon.
- Hypocritical Humor: Sylvester's sponsor mouths all the BA platitudes but breaks down instantly after kissing Tweety, at which point Sylvester tries to tell him to get a hold of himself and tries to restrain him.
- The Insomniac: Sylvester can't sleep because all he can think about is eating Tweety.
- Meat-O-Vision: Sylvester has a vision of Tweety as a cooked bird.
- Nightmare Face: Sylvester when he is breaking down.
- No Name Given: The sponsor. Though he is referred to as "Clarence" or "Sam" in other Looney Tunes media.
- No Sympathy: Tweety's final line (and thus the short's) is him dismissing the two cats struggling with their addiction (and Sylvester in the middle of one of the rare times when he's trying to protect him) as two "bad ol' puddy tats".
- Off the Wagon: Both Sylvester and (to the literally sobering surprise of the former) his sponsor.
- The Remake: An episode of The Sylvester and Tweety Mysteries reused the Clarence character and the cold turkey motif; the difference was that it took place at a monastery where a cult of cats take a vow not to eat birds. Sylvester has just a hard of time sticking to that rule as in Birds Anonymous.
- Ripped from the Headlines: The short came out around the time Alcoholics Anonymous was gaining momentum.
- The Sponsor: Sylvester has one who tries to keep him on the straight path.
- Shout-Out: The short is a spoof of the gritty "message films" popular at the time, down to its unusually dark tone.
- Sting: One plays when Sylvester screams during his insomnia.
- Tropaholics Anonymous: An obvious joke based on Alcoholics Anonymous.
- Your Television Hates You: And your radio too. Sylvester tries to distract himself from Tweety by watching TV, only to see a chef cooking turkey; he turns on the radio and the playlist features "Bye-Bye, Blackbird" and "When the Red Red Robin Comes Bob Bob Bobbin' Along."(Fake Italian accent) "After-a basting, you find your bird turns out golden-a brown. Every succulent morsel will simply melt-a in your mouth. See how easily the white-a meat-a slices, eh? Yum, yum! Doesn't that-a look..."