Babs Bunny wants to become a famous star. She wants to practice at the Acme Theater, but Montana Max wants to buy the theater so he can tear it down and build a factory. Buster, Plucky, and Hamton have to help Babs fulfill her destiny and defeat Montana Max.
The game is a platformer with four levels, where the player can play as Buster, Plucky, or Hamton, each with their own projectiles. In each level, the character will come across a special helper who can assist with getting through a tight spot. Gems can be collected and spent playing mini-games for more power-ups or extra lives.
A sequel, Tiny Toon Adventures 2: Montana's Movie Madness followed in 1993.
This game provides examples of:
- Animated Armor: A knight's suit of armor serves as the boss of the Haunted Forest.
- Anvil on Head: Certain clones of Roderick Rat toss anvils at your player if they get too close to them.
- Bald Women: At the end of the chase with Elmyra Duff, when Elmyra falls off the bridge, she loses her wig, which floats down after her.
- Be the Ball: During the battle with Arnold, if you try to attack Arnold when he isn't standing still, he will bounce you like a basketball and toss you in one of the hoops.
- Bee Afraid: Level 3 has bees as enemies. The best way to defeat them is to take out their hive first.
- Big Badass Rig: Near the end of Level 4, just before the final battle with Montana Max, Monty controls a giant monster truck that the player has to avoid while making it to the end of the stage.
- Big Boo's Haunt: Level 3 is a haunted forest filled with ghostly enemies. Fifi is captured by the Forest Ghost, and after you rescue her, you go inside the castle, where the boss is a knight.
- Blackout Basement: Perhaps a literal example; The basement of ACME Looniversity has a flickering light in an otherwise dark environment.
- Corrupt Corporate Executive: Montana Max wants to buy out the theater that Babs wants to perform at so that he can build a factory, and you still have to fight him even if you do manage to save up 500 gems to buy the theater from him.
- Damsel in Distress: In Level 3, Fifi is captured by the Forest Ghost, and to rescue her, you have to find the switch that opens her cage.
- Difficulty by Region: A downplayed example; the Japanese version of the game differs slightly in that it offers a password feature and unlimited continues (The American version has no password feature and only allows the player to continue twice). Other than that, there are virtually no differences.
- Disney Villain Death: At the end of the chase with Elmyra Duff, Elmyra falls off a bridge.
- The Dragon: The Wolverine (from the TV series episode, "Buster and the Wolverine") serves as this to Montana Max, being his bodyguard, holding Shirley hostage and planning to eat her. He is also the last boss before Monty himself.
- Edible Ammunition: Each character can (in addition to the standard Goomba Stomp) wield a form of edible ammunition for long-distance attacks: Buster shoots carrots in a ballistic arc, Hamton uses watermelons as bowling-balls for a ground-tracking attacks, and Plucky Duck throws pineapples which travel diagonally and bounce off walls.
- Feed It a Bomb: At the end of Level 1, Dizzy Devil is the boss of the ACME Looniversity cafeteria, and in order to defeat him, the player must feed Dizzy enough food to make him fall asleep. While Dizzy will eat anything, it is best to feed him chicken and fish, as urchins can hurt your character.
- Goomba Stomp: Your character's basic method of attacking enemies is by jumping on them.
- Green Hill Zone: The first part of Level 1 takes place in ACME Acres.
- Hearts Are Health: The player starts out with two hearts. A little heart will add one heart to their health bar, while a big heart will extend it up to three. Completing a level will reset your health bar back to two hearts. Playing on Hard Mode will subtract one heart from your health bar.
- Hopping Machine: Montana Max controls one in the final battle against him.
- Locomotive Level: Near the end of Level 2, your character rides a train to Arnold's Gym.
- The Lost Woods: Much of Level 3 takes place in a dark forest.
- Money for Nothing: A downplayed example; just before the final battle with Montana Max, if the player has 500 or more gems, Monty will offer to sell the theater to the player. However, buying the theater will only result in an alternate dialogue sequence, as you'll still need to fight Monty.
- One-Hit-Point Wonder: Your character becomes this in Hard Mode.
- Pipe Maze: The first part of Level 2 is this.
- Public Domain Soundtrack: "Flight of the Bumblebee" is used as Dizzy Devil's theme, "In the Hall of Mountain King" is used in the Haunted Forest, and "Bizet" from Carmen is used during the race mini-game and Elmyra's chase scene.
- Racing Minigame: One of the mini-games is a racing mini-game, where your character can choose to race against Bookworm (slowest), Sweetie (moderate), or Little Beeper (fastest). tapping the A and B buttons simultaneously repeatedly makes your character run. Outracing the opponent will earn you a bonus.
- Smelly Skunk: Fifi La Fume. When rescued, she can use her odor to knock down the trees blocking the path in the haunted forest.
- Suddenly Voiced: While The Wolverine spoke mostly in grunts and roars, he speaks fluently to the player when he explains that he has captured Shirley and is planning to eat her.
- Whack-a-Monster: The "Montana Mash" mini-game involves your character hitting as many Montana Max and Roderick Rat targets as they can within the time limit. Each character wears different attire and whacks with a different object; Buster is dressed as Biff Vanderbunny and whacks with a cane, Plucky is dressed like a superhero and whacks with a hammer, and Hamton is dressed in his normal attire and whacks with a frying pan.