Not to say that Mario and Luigi don't show up in these games, because they do, usually as the Butt Monkeys. There's also the blatant trademark infringement on Nintendo characters, though that might also qualify for a fair use exemption as parody.
- Artistic License Biology: In New Super Chick Sisters Nugget and Chickette have mustaches... despite being chickens... and female.
- Author Tract: It's PETA, so of course the game would essentially be a long-winded platitude that boils down to "Fast food businesses are bad because they torture and kill animals."
- Big Bad:
- In the original game, Colonel Sanders kidnaps Pamela Anderson to stop her from exposing the truth of his business which tortures chickens.
- In New Super Chick Sisters, Ronald McDonald has Pamela kidnapped to keep his corrupt practices under wraps.
- Bound and Gagged: Pamela Anderson is tied up before you rescue her.
- Butt-Monkey: The Super Mario Bros., especially Mario, are subjected to frequent misfortunes in the first game.
- Disproportionate Retribution: Mario suffering "Wiitis" and getting slapped by Princess Peach over a photo of Pamela Anderson are tame compared to getting beat up by People for the Ethical Treatment of Turtles protesters for stomping on Koopas.
- Enemy Mine: The Mario Bros. enlist Bowser's help in New Super Chick Sisters.
- Gender Flip: Nugget is traditionally a male mascot, but these games depict him as female.
- Girls With Mustaches: Chickette and Nugget earn small mustaches in the second game despite being female.
- Informed Attractiveness: Pamela Anderson is inferred as being the most beautiful woman in the world. While she is certainly Ms. Fanservice in real life, one can't really tell by the game's goofy, cartoony art style.
- Monster Clown: Ronald McDonald is depicted as a hideous clown in New Super Chick Sisters.
- Off-Model: The designs of Luigi and Bowser are based on how they appeared in Paper Mario and Mario & Luigi: Bowser's Inside Story respectively, though they are not accurate. Mario and Peach are derived from their Paper Mario appearances as well, and are mostly accurate, but their backsides are another story.
- Self-Parody: PETA gets parodied by themselves in a cutscene where the People for the Ethical Treatment of Turtles (or PETT for short) appear and beat up Mario for jumping on Koopa Troopas, even though these turtles do try to kill him.
- Take That!: Poorly disguised as Affectionate Parody. Making a joke out of the number of Mario Sports titles is one thing, going out of your way to reference Mario Paint and Mario Teaches Typing as Shovelware is another.
- The Unfought: The Hamburglar is shown kidnapping Pamela Anderson in the opening cutscene of New Super Chick Sisters, but he does not get his own boss fight and just disappears from the story afterwards.
- You Don't Look Like You: Ronald McDonald's character design changes between his first appearance and the boss battle.