- Channel Hop: The original series was recored in Funimation's own dubbing studio. On the other hand, its sequel, UQ Holder, was recorded at Seraphim Digital Studios and licensed by Sentai Filmworks.
- Crossdressing Voices: Touta Konoe is voiced by voice actresses Yuka Takakura in the original Japanese version and Brittney Karbowski in the English dub. Rina Sato reprises her role as Negi Springfield.
- Relationship Voice Actor: some of the cast of the Original Mahou Sensei Negima! and its sequel had worked together in other anime, to elaborate:
- Negima!Setsuna and UQ Holder!Setsuna are Yoshika and Mio and Lucy and Ur, though Ur died before Lucy met her.
- Negima!Akira and UQHolder!Akira are Mutsuki and Yamato.
- Negima!Mana and UQ Holder! Mana (Katelyn Barr) are young Gaku and Miyako.
- Negima! Ku Fei and UQ Holder!Ku Fei are female Pipimis in episodes 10 and 11.
- Negima!Misa (Gwendolyn Lau) and UQ Holder!Misa are Sheska and Xiao Mei, Vasant and Alvis and Daphne and Happy.
- The Other Darrin: Averted for all returning Negima characters. They all retain their original Japanese voices, with the sole exception of Chisame (Daria Midou replacing Yumi Shimura). Played straight with the dub with the exceptions of Greg Ayres (Negi), Luci Christian (Asuna), and Monica Rial (Konoka and Kazumi, though both of them were only cameos), but this is mainly because many of them have moved to Los Angeles (mostly Chris Patton, Cherami Leigh, and Laura Bailey), which makes it harder for Sentai Filmworks to have their old voice actors reprise their roles.
- Trolling Creator: Ken Akamatsu manages to continue his habit of trolling his own publishers, with UQ Holder being a stealth sequel to Negima for over a hundred chapters, with the original cast being seemingly Killed Off for Real... only for that very first page to be a Fake-Out Opening of epic proportions, as recent chapters brought back a sizeable proportion of the fan favourites from the original series, culminating in Chapter 129, where it gains a new title... Mahou Sensei Negima 2: UQ Holder. The series isn't over yet.
Trivia / UQ Holder!