- Actor-Inspired Element: The exploration of Bette's mixed race parenting was suggested by Jennifer Beals, whose own heritage is really important to her.
- ...But I Play One on TV: Mia Kirshner told a story about how once when she was with some friends in a club in Vancouver, another girl came up to her and said "I hope you die." Presumably she was talking about Jenny Schecter. Mia however would have none of that and took the woman aside and explained, she (like the woman) was here to have fun, and she worked to make money. Jenny was a fictional character, and it was fine it she wanted her to die. It was not okay to want Mia herself to die, because that was hurtful. The woman was taken aback.
- California Doubling: Inverted - The series took place in Los Angeles, but was filmed in Vancouver. Lampshaded at one point in Season 5, when Jenny is scouting locations for Lez Girls, and loudly proclaims that "Vancouver can't pass for L.A.!"
- Contractual Purity: Jennifer Beals is one of the only main characters whose bare breasts are never shown throughout the entire series.
- Creator Backlash:
- Mia Kirshner said in an interview that she was sad seeing how her character ended up being a lunatic and a stereotype.
- Even though she doesn't regret telling the story, Ilene Chaiken admitted on an interview that if she had known how much anger the death of Dana as a result of breast cancer was going to rise in people, she would have done it differently.
- Lucy Lawless revealed in an interview that when shooting the scenes where her character questioned the women of the show about Jenny's death, the actresses were openly unhappy with the decision of killing the character and making the storyline into a possible murder mystery, with them as suspects.
- Creator Breakdown: After Dana dumps Alice between Seasons 2 and 3, Alice's radio show begins to suffer. At one point, a commercial for Alice's show plays on a nearby radio, consisting of little more than Alice screaming Dana's name at the top of her lungs.
- Executive Meddling: In-setting, this trope comes into play at least once a season, costing just about every character their job at some point, and totally ruining Lez Girls.
- Hide Your Pregnancy:
- Jennifer Beals was pregnant with her daughter, Ella during the third season. This was covered by having her wear powersuits.
- Averted when Laurel Holloman became pregnant during the second season.
- Hostility on the Set: Kristanna Loken asked to be written off the series because she was overwhelmed by the mainly female crew:It's an entire show of women and the amount of egos and insecurities and cattiness that can go with a bunch of women - that's what you get. It attracts certain people that want to do something different in their career, but the girls were tough. Sometimes you wonder if they've bit off more than they can chew.
- In Memoriam: Ossie Davis had a recurring role as Bette's father. In the second season, Davis filmed scenes that showed his character becoming ill, then dying. Davis then died before his final episode was broadcast, so that episode was dedicated to him.
- Post-Script Season: Season 6.
- Queer Character, Queer Actor: Laurel Holloman, Leisha Hailey, Clementine Ford, Tammy Lynn Michaels, Guinevere Turner, Jane Lynch, Alexandra Holden, Kelly McGillis, Kristanna Loken, Daniela Sea, Karina Lombard and Heather Matarazzo are all lesbian or bisexual in real life, as well as playing lesbian or bisexual characters on the show (and Mia Kirshner has hinted at bisexuality in interviews).
- Role-Ending Misdemeanor: Scott Bairstow was originally cast as the male lead, but was replaced by Eric Mabius soon after allegations of sexual assault on a minor were made against him in 2003 (he pleaded guilty to the lesser charge of second degree assault on 9 December 2003).
- Throw It In!: The scene in season 2 episode 1 where Tina and Bette are at the planet and Tina throws the table towards Bette wasn't scripted. She was in fact suppose to throw a coffee cup or plate, not the table.
- What Could Have Been:
- Written-In Infirmity: Tina's pregnancy in season two was added when Laurel Holloman became pregnant.
Trivia / Thelword