- Actor-Shared Background: Flynn mentions being from Tennessee, which is also where Samuel L. Jackson grew up
- Awesome, Dear Boy: Sam Jackson stated in interviews that he signed up just because of the title. He also threatened to quit when the producers wanted to rename it.
- Completely Different Title:
- Argentina: Terror on Board
- Japan: Snake Flight
- Russia: Serpentine Flight
- Turkey: Killer Snakes
- Venezuela: Terror in the Air
- Fake American: The Australian Nathan Philips does an American accent to play Sean Jones.
- Not Screened for Critics: It's not for them. The funny thing about this one is that the critical reaction was favorable. The film actually holds a 68% critic rating on Rotten Tomatoes, but only 50% audience. The critic consensus was summed up as: "Snakes on a Plane lives up to its title, featuring snakes on a plane. It isn't perfect, but then again, it doesn't need to be."
- Permanent Placeholder: The movie was going to be renamed Pacific Air Flight 121 for its release, but then its B-movie working title attracted a flurry of attention on the internet, and Samuel L. Jackson insisted that he'd only signed on to the film because of its Exactly What It Says on the Tin title. The producers kept the working title to cash in on the online hype.
- Technology Marches On: When Mercedes suggests they e-mail the herpetologist photos of the snakes they've killed so he can identify them, everyone else assumes they need to find a digital camera and computer. She holds up her smartphone and tells them it has both, implying that she is the only one on board who has such a phone because she is rich.
- Throw It In!: The famous "I have had it..." line originated in a parody made in anticipation of the movie's release. The filmmakers added it into the final cut right before the release.
- Unintentional Period Piece: The ability to take photos with a cell phone, and send them automatically to someone else was somewhat new at the time - and Mercedes is one of the few who can because she's implied to be a rich Valley Girl. So, the scene where everyone has to be reminded that they can take digital photos of the snakes and send them to a snake expert can seem a bit jarring to younger fans.
- Vindicated by Cable: It's not the kind of movie you should watch in a quiet theater. It's the type of movie you should watch at home with friends as you laugh and snark the hell out of it. The campiness of some of the TV edits also helps boost the snark factor for some.
- What Could Have Been:
- The initial script was 122 pages long, but got narrowed down to 103 pages over a period of four months.
- The first cut of the movie was going to be PG-13. But after fan interest on the internet, they did five days of re-shoots to add some R-rated content in.
Trivia / Snakes on a Plane