- Billing Displacement: Jill replaces Sheva on the cover artwork of the Gold Edition, despite the fact that she's only playable in the two unlockable chapters and doesn't show up in the main campaign until the end.
- Fake Nationality: Josh Stone, who speaks with an African accent, is voiced by TJ Storm, an American.
- Fan Nickname: Chris is referred to as "Chris Roidfield", thanks to his bulky appearance.
- Let's Play: One particularly good co-op Let's Play was done by ProtonJon and Super Jeenius here.
- Name's the Same: Music-wise, Jill's boss theme, "Sad But True", is also the name of songs by Metallica and Orbital.
- What Could Have Been: Not to the extent of 4, but nevertheless the game underwent a number of changes before release, many which are detailed in The Art of Resident Evil 5:
- The game was originally designed as a single-player game akin to 4, with Chris as the only playable character. Sheva was originally intended to be a recurring character, being the head of a local militia fighting against the outbreak. When the decision was made to make the game support two-player cooperative, Sheva was rewritten as a BSAA operative and Chris's partner.
- Jill was originally to be found frozen and used by Wesker for research in the TRICELL facility, but it was decided it wasn't interesting enough.
- Barry Burton was meant to appear multiple times during the game, at one point assisting in a boss fight against no less than ten El Gigantes. He ended up cut from the storyline completely, though he would be a playable character in The Mercenaries Reunion in the Gold Edition.
- Excella would have simply been a secretary to Tricell, and her death was portrayed very differently (she would have died in the hands of a Tyrant-like creature, literally), and the Tricell CEO would have been a different, male character. The two characters were ultimately combined in order to create a powerful female villain.
- A placeholder model for a merchant character was found in the files of the game, suggesting that the Merchant from 4 was intended to return at some point in development. Also found in the game files was a player model version of Ricardo Irving, suggesting he had been intended to be a playable character for The Mercenaries mode.
- An early Famitsu interview with producer Jun Takeuchi stated that the use of light would be very important in the game. The example given was that if the player character were to move from a bright area to a dark area very quickly, it would take them a few moments to adapt before they could see again, an effect that would also be replicated for the player. The opposite effect of moving into a bright area from a dark one would work similarly. These moments of inability to aim or see would be used to create moments of fear in the game. In the final version, however, these light mechanics seem to have been entirely scrapped.
Trivia / Resident Evil 5