- Awesome, Dear Boy: You may think a Hollywood legend like Roddy McDowall would resent being in cartoons, but as it turns out he loved playing Snowball and got on very well with Maurice and Rob.
- Biting-the-Hand Humor: The episode "You'll Never Eat Food Pellets in This Town Again", which features the title lab mice as the stars of a hit TV show being heavily meddled with by network executives, who think heavily altering the show's premise will increase ratings. (See Pinky, Elmyra & the Brain.) The iTunes description for this episode even referred to it's head of the network character Sandy Dreckman as Jamie Kellner, the then ACTUAL head of the WB Network.
- The Character Died with Him: Sort of. Snowball's last appearance, in which he was turned into a regular hamster was aired around the time of his voice actor's death. In essence, Snowball has been defeated for good.
- Completely Different Title: Know in French as Minus et Cortex, which uses the same "X & Y" structure and order as the original title, but with the French translation of the characters' names ("Minus" means either "idiot" or "weakling", while "Cortex" is a part of the brain.)
- Edited for Syndication: When the show aired on Nickelodeon and its sister networks, they used an altered version of the theme song that had the Nick logo plastered onto random places throughout the theme song, almost egregiously so. Viewers who have seen this variant will often jokingly ask "what network was this again?"
- Fake Brit: Rob Paulsen as Pinky.
- Franchise Killer: What happened when Pinky, Elmyra & the Brain came in.
- Unintentional Period Piece: The show frequently pokes fun at 90s politics and pop culture, some obscure. For example, "Pinky Suavo" is one long reference to the 1991 single "Rico Suave."
- What Could Have Been: The show was slated to air on The Hub in late 2014, but due to the change to Discovery Family, it never came to be.
- There was a planned Pinky and the Brain video game for the Sega Saturn, and possibly the PlayStation and N64 but it was canceled and there is little to no information about the game. The little info can be seen here on page 75.
- Russell Calabrese and Kirk Tingblad once proposed a Pinky and the Brain movie. The idea was to have an experiment fail and they get turned into their voice actors, Rob Paulsen and Maurice LaMarche. The two go on a world wind adventure to find a certain gizmo that Brain can fix their conversion machine with. They find the gizmo they need and race to the lab to rebuild the device to return them into mice. Just as they start up the device Pinky is shot with the bullet hitting his heart. The conversion happens and as Brain is sobbing the bullet rolls off his cartoon chest and Pinky is saved. It ends with them cleaning up the lab ab Brain asks Pinky "Are you pondering what I am pondering?". It was rejected as being too expensive. Source: https://m.facebook.com/story.php?story_fbid=2148146041911970&id=100001497862192
- Writer Revolt: "You'll Never Eat Food Pellets in This Town Again", "Pinky and The Brain... and Larry!" and the opening theme to Pinky, Elmyra & The Brain.
- Writing by the Seat of Your Pants: According to one of the head writers, Charles M. Howell, each of the 52 half hour episodes only had one week to prepare a script for them. Despite this, they ended up going four months over schedule.
Trivia / Pinky and the Brain