- Accidentally Accurate: In the movie, Barry is upset to learn that bees' honey is being harvested in beekeeper colonies. In real life, there's actually a controversy over extracting honey from beehives due to a common beekeeper practice being to replace the honey with sugar syrup, as sugar syrup is not as nutritional for bees as honey is.
- Actor-Shared Background: Like Seinfeld, Barry has an expansive collection of sneakers (Seinfeld is rumored to have over 500).
- Celebrity Voice Actor: Aside from Jerry Seinfeld and Renée Zellweger, there's Patrick Warburton as Ken, Matthew Broderick as Adam Flayman, John Goodman as Layton T. Montgomery, Chris Rock as a mosquito and Oprah Winfrey as the judge.
- Prop Recycling: The grizzly bear seen in a couple of scenes (most prominently when Barry has an animal control officer bring it into the courtroom so he can prove bears have a negative reputation among bees) reuses Vincent's CGI model from Over the Hedge, albeit with brown-colored fur.
- The Other Darrin: Renée Zellweger and Matthew Broderick did not reprise their roles as Vanessa and Adam for the tie-in game, being replaced with Melissa Disney and Jason Harris Katz.
- The Red Stapler: People actually looked for Ray Liotta Honey after the movie came out.
- Talking to Himself: Jim Cummings voices the narrator, the voice over for the New Hive City college and Winnie the Pooh.
- What Could Have Been:
- The unseen queen bee of the hive was originally planned to appear as a character in the movie, shown in a series of storyboards in which she brings Barry to her quarters to chastise him for violating the "no contact with humans" law. Her design is drastically different from the rest of the bees, being far more curvaceous and human-like in appearance◊ compared to the more cartoonish, insect-like proportions of her subjects.
Trivia / Bee Movie