- The Cast Showoff: Melissa Rauch gets her own musical number as Harley. Rob Paulsen also gets one, which sees him singing a duet with himself, due to playing the twins from Batman: The Animated Series episode "Two-Face".
- Recycled Script: The movie's plot is pretty similar to the plots of the BTAS-episodes "Harley's Holiday" and "Harlequinade", especially the latter, with Poison Ivy and Jason Woodrue replacing Joker.
- Role Reprise:
- Loren Lester as Nightwing, returning to this role since the end of The New Batman Adventures era, having last played the role in the 2003 video game Batman: Rise of Sin Tzu. Kevin Conroy once again playing Batman is a very lesser example since that man was everywhere.
- In the French dub, Kelvine Dumour as Harley Quinn from the DC Animated Universenote , Mathias Kozlowski as Nightwing from Teen Titans, Emmanuel Jacomy as Batman from Justice League vs. Teen Titansnote , Dominique Vallée as Poison Ivy from Justice League: Gotham City Breakoutnote and Saïd Amadis as Swamp Thing from Justice League Darknote .
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