WARNING: Unmarked Spoilers Ahead.
- The ending of season 1 episode 5. Nice Job Breaking It, Hero!.
- Brian getting expelled in season 1 episode 7. The whole ending of that episode, really.
- In season 2 episode 5, Shot Bot's Heroic Sacrifice to save the Law and the data that can clear his name. Made worse in the next episode, when Scott Slanders refuses to accept "Shot Bot 2.0" and laments how he's lost his best friend before outright leaving.Scott: My best friend is gone.
- The Law goes from Moment of Awesome to Funny to this in seconds. At the start of episode 6 of season 2, he's seen riding a motorcycle, looking determined to get somewhere...then cut to him at a fast food joint, trying to order through the drive-thru but being refused due to being on a motorcycle. Then he breaks down crying and admits that he's lost, has no idea how to get home, and just lost his best friend. It's sad, but it arguably becomes funny as the people behind him start honking their horns at him.
- The moment at the end of the second season: Brian, after Jenny confesses that she loves him and making out with her, returns to his room...to find that Ted's stuff, as well as Ted himself, is gone, having apparently moved out.
- Jenny's speech in Episode 3 is heartbreaking, as well as every time Brian and her talk about it. Consider Brian's advice and his relationship with his own mom...
- When they first talk about the speech, and she breaks down upon realizing that she doesn't have anything positive to say about her mother.
- In the first episode of season 3, Ted and Brian go on a quest in the collector's edition of a game called Deathstalkers to obtain the Babbage's Blade, which they need to get for Ki, who is running for student president, so she can win the votes of the MMO guild. Everything is going swimmingly until the very end of the quest, when Brian picks up the blade and unknowingly binds himself to it (meaning he can't give it to Ted). An argument ensues in-game, and it then carries on into the real world where Ted and Brian end up in a fight that results in Ted getting a serious nosebleed. Even though Brian tries to apologize, Ted refuses to accept his apologies, says that he is "done", denounces their friendship, and tells Brian to go to hell. Utterly tragic.
- Season 3 episode 3 may very easily be the most depressing episode in the entire show. Where do we begin?
- Jenny ended up costing her team a match against Napalm and Brian breaks up with her immediately after the game.
- Knowing that her chances of becoming student president are very slim, Ki is resorted to stuffing the ballot in order to win, but she holds the presidency for a good minute or so before finally coming clean to both her team and Calhoun.
- "Ki left school. She's gone." Ted's look of anguish and heartbreak, paired up with Brian's look of shock and disbelief as he receives the news...
- Ted blackmails the MMO guild with a Photoshopped picture of their leader's Tomowachi (appearing to be alive, even though it's dead IRL).
- Season 3 Episode 4, considering the whole driving force of that episode is Freddie's death in a motorcycle accident (and the fact that he wasn't wearing his helmet may have been another contribution), and how Ted copes with it. At first, he doesn't seem to be affected that much, but as the episode progresses his realization of the toll his father's death has taken on him causes Ted to fall into the Despair Event Horizon. To make matters worse, when everyone watches Freddie's last will (which came in a video form), we see Freddie berating Ted on camera and bequeathing him a crappy microphone while he gives his most valuable possessions to someone he never met. The icing of the cake is at the end of the episode, where Jenny, Brian, and Ki manage to find Ted late at night at a laundromat playing Axe Legends, having beat the game countless times just to hear his dad compliment him at the end. Afterward, Ted finally breaks down, having realized how much of an asshole his dad was to him.
- Picture this: after having gone on a rant about his dad, Ted starts to weep. After Ki tells him that it's okay to cry, Ted just starts bawling and collapses to the floor.
- "Nothing is alright."
- What makes the episode so powerful is that it has one of the most realistic portrayals of someone's reaction to the loss of a loved one of all time. Mix this with the acting power of the leads, and you have some chill-inducing stuff.
- Seeing Ki break down in tears with her parents about cheating to win the election was also pretty devastating.
- In season 3 episode 5:
- We see just how much of a toll losing the election took on Ki. She goes from a peppy and kind-hearted to nihilistic and moody, almost as if she's a completely different person. She does snap out of it by the end, though.
- In the same episode, we also hear that Brian never truly had any parents that took care of him—his mom was an addict, and he never really had a dad. He had to spend his childhood taking care of himself, and he states that as a result he got used to being alone.
- "I am an angry, unhappy person. That's probably why I punched Ashley in the face, and why I have no real friends, and why I just ruined my relationship with the only person in my life that's ever really loved me." - Jenny
- The opening scene of the finale. We see everyone packing up their stuff as the Napalm team renovates on the school and a sad song performed by Raul Panther of The Protomen plays as BGM. Even Calhoun is depressed.
- At the end of the FPS team training (to go up against Napalm), Jenny gives a little speech about how she knows the team will probably lose and that the school will be gone by the next day, but that she doesn't want to leave the school and is at least grateful to spend her last day playing with the team.
- Ted's Go Out with a Smile in the match against Napalm. "I love you all."